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Single-stranded DNA-binding protein

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[[Image:1eqq.png|left|200px|thumb|Crystal structure of SSB complex with ssDNA [[1eqq]]]]
 
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{{STRUCTURE_1eqq| PDB=1eqq | SIZE=350| SCENE= |right|CAPTION=SSB complex with ssDNA [[1eqq]] }}
 
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<StructureSection load='1eqq' size='350' side='right' scene='' caption='E. coli SSB complex with ssDNA (stick model) and UMP [[1eqq]]'>
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'''Single-stranded DNA-binding protein''' (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities<ref>PMID:22976174</ref>.<br />
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* '''Viral SSB proteins''' are monomeric.<br />
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* '''Eukaryotic SSB''' ('''Replication protein A''' – RPA) is a heterotrimer composed of RPA70, RPA32 and RPA14. RPA has 6 DNA-binding domains (DBD).<br />
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*'''Replication protein A70''' is the eukaryotic SSB 70kDa subunit<ref>PMID:11470540</ref>.
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* '''Mitochondrial human SSB (HsmtSSB)''' is a homotetramer.
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See also [[Single stranded binding protein]].
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'''Single-stranded DNA-binding protein''' (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities.
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== 3D Structures of single-stranded DNA-binding protein ==
== 3D Structures of single-stranded DNA-binding protein ==
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[[Single-stranded DNA-binding protein 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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[[1gpc]] – SSB gp32 – Enterobacteria phage T4 <br />
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[[2a1k]] – SSB gp32 – Enterobacteria phage RB69 <br />
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[[1je5]] – SSB – Enterobacteria phage T7 <br />
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[[1adu]], [[1adv]], [[1anv]] – SSB C terminal – human adenovirus 5 <br />
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[[1urj]] – SSB – human herpesvirus 1 <br />
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[[1pfs]] – SSB (mutant) – Pseudomonas phage PF3 - NMR<br />
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[[3ull]], [[1s3o]], [[2dud]] – SSB – human <br />
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[[1kaw]] – EcSSB chymotryptic domain – ''Escherichia coli'' <br />
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[[1qvc]], [[1sru]] – EcSSB <br />
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[[1o7i]] – SSB residues 1-119 – ''Sulfolobus solfataricus''<br />
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[[1ue1]], [[1ue5]], [[1ue7]] – SSB – ''Mycobacterium tuberculosis'' <br />
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[[1x3e]], [[1x3f]], [[1x3g]] – MsSSB – ''Mycobacterium smegmatis'' <br />
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[[1se8]] – DrSSB – ''Deinococcus radiodurans'' <br />
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[[4exw]] – SSB DdrB – ''Deinococcus geothermalis'' <br />
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[[1z9f]] – SSB – ''Thermotoga maritima'' <br />
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[[2cwa]] – SSB – ''Thermus thermophilus'' <br />
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[[2fxq]], [[2ihe]] – TaSSB – ''Thermus aquaticus'' <br />
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[[2ihf]] – TaSSB (mutant) <br />
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[[2hql]] – SSB – ''Mycoplasma pneumoniae'' <br />
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[[3eiv]] – ScSSB – ''Streptomyces coelicolor''<br />
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[[4dam]] – ScSSB (mutant) <br />
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[[3lgj]], [[3pgz]] – SSB – ''Bartonella henselae'' <br />
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[[3afp]], [[3afq]] – SSB – ''Mycobacterium leprae'' <br />
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[[3tqy]] – SSB – ''Coxiella burnetii'' <br />
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[[3tek]] – SSB N terminal – ''Thermoproteus tenax''<br />
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[[4gs3]] – SSB N terminal – ''Thermoanaerobacter tengcongensis''<br />
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===SSB complex with ssDNA===
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[[1eyg]] – EcSSB chymotryptic domain + ssDNA <br />
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== References ==
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[[1eqq]] – EcSSB + ssDNA <br />
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<references/>
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[[2vw9]] – HpSSB + ssDNA <br />
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[[3vdy]] – SSB + ssDNA – ''Bacillus subtilis''<br />
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[[3a5u]] – MsSSB chymotryptic domain + ssDNA <br />
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[[3udg]] – DrSSB + ssDNA <br />
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[[Category:Topic Page]]

Current revision


E. coli SSB complex with ssDNA (stick model) and UMP 1eqq

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References

  1. ↑ Marceau AH. Functions of single-strand DNA-binding proteins in DNA replication, recombination, and repair. Methods Mol Biol. 2012;922:1-21. doi: 10.1007/978-1-62703-032-8_1. PMID:22976174 doi:http://dx.doi.org/10.1007/978-1-62703-032-8_1
  2. ↑ Ishibashi T, Kimura S, Furukawa T, Hatanaka M, Hashimoto J, Sakaguchi K. Two types of replication protein A 70 kDa subunit in rice, Oryza sativa: molecular cloning, characterization, and cellular & tissue distribution. Gene. 2001 Jul 11;272(1-2):335-43. PMID:11470540 doi:10.1016/s0378-1119(01)00555-8

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Michal Harel, Alexander Berchansky

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