Single-stranded DNA-binding protein

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[[Image:1eqq.png|left|200px|thumb|Crystal structure of SSB complex with ssDNA [[1eqq]]]]
 
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{{STRUCTURE_1eqq| PDB=1eqq | SIZE=350| SCENE= |right|CAPTION=SSB complex with ssDNA and UMP (stick model) [[1eqq]] }}
 
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<StructureSection load='1eqq' size='350' side='right' scene='' caption='E. coli SSB complex with ssDNA (stick model) and UMP [[1eqq]]'>
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'''Single-stranded DNA-binding protein''' (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities<ref>PMID:22976174</ref>.<br />
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* '''Viral SSB proteins''' are monomeric.<br />
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* '''Eukaryotic SSB''' ('''Replication protein A''' – RPA) is a heterotrimer composed of RPA70, RPA32 and RPA14. RPA has 6 DNA-binding domains (DBD).<br />
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*'''Replication protein A70''' is the eukaryotic SSB 70kDa subunit<ref>PMID:11470540</ref>.
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* '''Mitochondrial human SSB (HsmtSSB)''' is a homotetramer.
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See also [[Single stranded binding protein]].
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'''Single-stranded DNA-binding protein''' (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities. Viral SSB proteins are monomeric while eukaryotic SSB ('''Replication protein A''' – RPA) is a heterotrimer composed of RPA70, RPA32 and RPA14. RPA has 6 DNA-binding domains (DBD). The '''mitochondrial human SSB (HsmtSSB)''' is a homotetramer. See also [[Single stranded binding protein]].
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== 3D Structures of single-stranded DNA-binding protein ==
== 3D Structures of single-stranded DNA-binding protein ==
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[[Single-stranded DNA-binding protein 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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[[1gpc]] – SSB gp32 – Enterobacteria phage T4 <br />
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[[2a1k]] – SSB gp32 – Enterobacteria phage RB69 <br />
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[[1je5]] – SSB – Enterobacteria phage T7 <br />
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[[1adu]], [[1adv]], [[1anv]] – SSB C terminal – human adenovirus 5 <br />
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[[1urj]] – SSB – human herpesvirus 1 <br />
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[[1pfs]] – SSB (mutant) – Pseudomonas phage PF3 - NMR<br />
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[[3ull]], [[1s3o]], [[2dud]] – HsmtSSB residues 16-148 – human <br />
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[[1kaw]] – EcSSB chymotryptic domain – ''Escherichia coli'' <br />
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[[1qvc]], [[1sru]] – EcSSB <br />
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[[1o7i]] – SSB residues 1-119 – ''Sulfolobus solfataricus''<br />
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[[1ue1]], [[1ue5]], [[1ue7]], [[1ue6]] – SSB – ''Mycobacterium tuberculosis'' <br />
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[[1x3e]], [[1x3f]], [[1x3g]] – MsSSB – ''Mycobacterium smegmatis'' <br />
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[[1se8]] – DrSSB – ''Deinococcus radiodurans'' <br />
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[[4exw]] – SSB DdrB – ''Deinococcus geothermalis'' <br />
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[[1z9f]] – SSB – ''Thermotoga maritima'' <br />
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[[2cwa]] – SSB – ''Thermus thermophilus'' <br />
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[[2fxq]], [[2ihe]] – TaSSB – ''Thermus aquaticus'' <br />
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[[2ihf]] – TaSSB (mutant) <br />
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[[2hql]] – SSB – ''Mycoplasma pneumoniae'' <br />
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[[3eiv]] – ScSSB – ''Streptomyces coelicolor''<br />
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[[4dam]] – ScSSB (mutant) <br />
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[[3lgj]], [[3pgz]] – SSB – ''Bartonella henselae'' <br />
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[[3afp]], [[3afq]] – SSB – ''Mycobacterium leprae'' <br />
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[[3tqy]] – SSB – ''Coxiella burnetii'' <br />
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[[3tek]] – SSB N terminal – ''Thermoproteus tenax''<br />
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[[4gs3]] – SSB N terminal – ''Thermoanaerobacter tengcongensis''<br />
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[[3dm3]] – SSB A domain – ''Methancaldococcus jannaschii''<br />
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[[1vqe]], [[1yha]], [[1vqb]] – F1SSB – Enterobacteria phage F1<br />
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[[2gn5]] – M13SSB – Enterobacteria phage M13<br />
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[[2gva]], [[2gvb]] – M13SSB (mutant) - NMR<br />
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[[1vqa]], [[1vqc]], [[1vqd]], [[1vqe]], [[1vqf]], [[1vqg]], [[1vqh]], [[1vqi]], [[1vqj]], [[1ae2]], [[1ae3]], [[1gkh]], [[1SSB]] – F1SSB (mutant) <br />
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===SSB complex with ssDNA===
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[[1eyg]] – EcSSB chymotryptic domain + ssDNA <br />
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[[1eqq]] – EcSSB + ssDNA <br />
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[[2vw9]] – HpSSB + ssDNA <br />
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[[3vdy]] – SSB + ssDNA – ''Bacillus subtilis''<br />
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[[3a5u]] – MsSSB chymotryptic domain + ssDNA <br />
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[[3udg]] – DrSSB + ssDNA <br />
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===SSB other complexes===
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[[3ufm]], [[3uf7]] – DrSSB C terminal + uracil-DNA glycosylase <br />
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===Replication protein A===
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''RPA70''
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[[1ewi]], [[2b29]] - hRPA70 N terminal - NMR<br />
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[[4ipc]], [[4ipd]], [[4ipg]] - hRPA70 N terminal (mutant) <br />
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[[1fgu]] – hRPA70 central domain <br />
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[[1ynx]] – yRPA69 DBD-A – yeast – NMR<br />
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[[1jmc]] – hRPA70 central domain + DNA <br />
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[[2b3g]] - hRPA70 N terminal + p53 peptide<br />
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[[4nb3]] - hRPA70 N terminal (mutant) + peptide<br />
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[[4ijh]], [[4ijl]], [[4iph]], [[4luo]], [[4luv]], [[4luz]], [[4lw1]], [[4lwc]], [[4o0a]] - hRPA70 N terminal (mutant) + inhibitor<br />
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''RPA14 + RPA32 + RPA70''
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[[1l1o]] – hRPA14 + hRPA32 central domain + hRPA70 C terminal <br />
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision


E. coli SSB complex with ssDNA (stick model) and UMP 1eqq

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References

  1. ↑ Marceau AH. Functions of single-strand DNA-binding proteins in DNA replication, recombination, and repair. Methods Mol Biol. 2012;922:1-21. doi: 10.1007/978-1-62703-032-8_1. PMID:22976174 doi:http://dx.doi.org/10.1007/978-1-62703-032-8_1
  2. ↑ Ishibashi T, Kimura S, Furukawa T, Hatanaka M, Hashimoto J, Sakaguchi K. Two types of replication protein A 70 kDa subunit in rice, Oryza sativa: molecular cloning, characterization, and cellular & tissue distribution. Gene. 2001 Jul 11;272(1-2):335-43. PMID:11470540 doi:10.1016/s0378-1119(01)00555-8

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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