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Single-stranded DNA-binding protein

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<StructureSection load='1eqq' size='450' side='right' scene='' caption='E. coli SSB complex with ssDNA (stick model) and UMP [[1eqq]]'>
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<StructureSection load='1eqq' size='350' side='right' scene='' caption='E. coli SSB complex with ssDNA (stick model) and UMP [[1eqq]]'>
'''Single-stranded DNA-binding protein''' (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities<ref>PMID:22976174</ref>.<br />
'''Single-stranded DNA-binding protein''' (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities<ref>PMID:22976174</ref>.<br />
* '''Viral SSB proteins''' are monomeric.<br />
* '''Viral SSB proteins''' are monomeric.<br />
* '''Eukaryotic SSB''' ('''Replication protein A''' – RPA) is a heterotrimer composed of RPA70, RPA32 and RPA14. RPA has 6 DNA-binding domains (DBD).<br />
* '''Eukaryotic SSB''' ('''Replication protein A''' – RPA) is a heterotrimer composed of RPA70, RPA32 and RPA14. RPA has 6 DNA-binding domains (DBD).<br />
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*'''Replication protein A70''' is the eukaryotic SSB 70kDa subunit<ref>PMID:11470540</ref>.
* '''Mitochondrial human SSB (HsmtSSB)''' is a homotetramer.
* '''Mitochondrial human SSB (HsmtSSB)''' is a homotetramer.
See also [[Single stranded binding protein]].
See also [[Single stranded binding protein]].
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</StructureSection>
 
== 3D Structures of single-stranded DNA-binding protein ==
== 3D Structures of single-stranded DNA-binding protein ==
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[[Single-stranded DNA-binding protein 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Single-stranded DNA-binding protein
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**[[1gpc]] – SSB gp32 – Enterobacteria phage T4 <br />
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**[[2a1k]] – SSB gp32 – Enterobacteria phage RB69 <br />
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**[[1je5]] – SSB – Enterobacteria phage T7 <br />
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**[[1vqe]], [[1yha]], [[1vqb]] – F1SSB – Enterobacteria phage F1<br />
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**[[1vqa]], [[1vqc]], [[1vqd]], [[1vqe]], [[1vqf]], [[1vqg]], [[1vqh]], [[1vqi]], [[1vqj]], [[1ae2]], [[1ae3]], [[1gkh]] – F1SSB (mutant) <br />
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**[[2gn5]] – M13SSB – Enterobacteria phage M13<br />
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**[[2gva]], [[2gvb]] – M13SSB (mutant) - NMR<br />
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**[[1adu]], [[1adv]], [[1anv]], [[2waz]], [[2wb0]] – SSB C terminal – human adenovirus 5 <br />
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**[[1urj]] – SSB – human herpesvirus 1 <br />
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**[[1pfs]] – SSB (mutant) – Pseudomonas phage PF3 - NMR<br />
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**[[3ull]], [[1s3o]], [[2dud]] – HsmtSSB residues 16-148 – human <br />
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**[[1kaw]] – EcSSB chymotryptic domain – ''Escherichia coli'' <br />
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**[[1qvc]], [[1sru]] – EcSSB <br />
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**[[1o7i]] – SsSSB residues 1-119 – ''Sulfolobus solfataricus''<br />
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**[[1ue1]], [[1ue5]], [[1ue7]], [[1ue6]] – SSB – ''Mycobacterium tuberculosis'' <br />
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**[[1x3e]], [[1x3f]], [[1x3g]], [[5gqo]] – MsSSB – ''Mycobacterium smegmatis'' <br />
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**[[1se8]] – DrSSB – ''Deinococcus radiodurans'' <br />
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**[[4exw]] – SSB DdrB – ''Deinococcus geothermalis'' <br />
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**[[1z9f]] – SSB – ''Thermotoga maritima'' <br />
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**[[2cwa]] – SSB – ''Thermus thermophilus'' <br />
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**[[2fxq]], [[2ihe]] – TaSSB – ''Thermus aquaticus'' <br />
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**[[2ihf]] – TaSSB (mutant) <br />
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**[[2hql]] – SSB – ''Mycoplasma pneumoniae'' <br />
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**[[3eiv]] – ScSSB – ''Streptomyces coelicolor''<br />
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**[[4dam]] – ScSSB (mutant) <br />
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**[[3lgj]], [[3pgz]] – SSB – ''Bartonella henselae'' <br />
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**[[3afp]], [[3afq]] – SSB – ''Mycobacterium leprae'' <br />
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**[[3tqy]] – SSB – ''Coxiella burnetii'' <br />
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**[[3tek]] – SSB N terminal – ''Thermoproteus tenax''<br />
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**[[4gs3]] – SSB N terminal – ''Thermoanaerobacter tengcongensis''<br />
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**[[3dm3]] – SSB A domain – ''Methancaldococcus jannaschii''<br />
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**[[5xgt]] – SSB N-terminal – ''Staphylococcus aureus''<br />
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**[[5m1x]] – ySSB N-OB domain (mutant) - yeast<br />
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**[[5d8e]], [[5d8f]] – hSSB residues 1-109 (mutant) - human<br />
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*Single-stranded DNA-binding protein complex with ssDNA
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**[[1eyg]] – EcSSB chymotryptic domain + ssDNA <br />
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**[[1eqq]] – EcSSB + ssDNA <br />
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**[[2vw9]] – HpSSB + ssDNA <br />
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**[[3vdy]] – SSB + ssDNA – ''Bacillus subtilis''<br />
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**[[3a5u]] – MsSSB chymotryptic domain + ssDNA <br />
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**[[3udg]] – DrSSB + ssDNA <br />
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**[[2mna]] – SsSSB + ssDNA - NMR<br />
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*Other single-stranded DNA-binding protein complexes
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**[[3ufm]], [[3uf7]] – DrSSB C terminal + uracil-DNA glycosylase <br />
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*Replication protein A70
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**[[1ewi]], [[2b29]] - hRPA70 N terminal - NMR<br />
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**[[4ipc]], [[4ipd]], [[4ipg]] - hRPA70 N terminal (mutant) <br />
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**[[1fgu]] – hRPA70 central domain <br />
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**[[1ynx]] – yRPA69 DBD-A – NMR<br />
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**[[1jmc]] – hRPA70 central domain + DNA <br />
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**[[2b3g]] - hRPA70 N terminal + p53 peptide<br />
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**[[5eay]] - hRPA70 N terminal + DNA2 peptide<br />
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**[[4nb3]], [[5n85]] - hRPA70 N terminal (mutant) + peptide<br />
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**[[5n8a]] - hRPA70 N terminal (mutant) + PrimPol<br />
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**[[4ijh]], [[4ijl]], [[4iph]], [[4luo]], [[4luv]], [[4luz]], [[4lw1]], [[4lwc]], [[4o0a]], [[4r4c]], [[4r4i]], [[4r4o]], [[4r4q]], [[4r4t]], [[5e7n]] - hRPA70 N terminal (mutant) + inhibitor<br />
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*Replication protein A70+A14+A32
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**[[1l1o]] – hRPA14 + hRPA32 central domain + hRPA70 C terminal <br />
 
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}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision


E. coli SSB complex with ssDNA (stick model) and UMP 1eqq

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References

  1. ↑ Marceau AH. Functions of single-strand DNA-binding proteins in DNA replication, recombination, and repair. Methods Mol Biol. 2012;922:1-21. doi: 10.1007/978-1-62703-032-8_1. PMID:22976174 doi:http://dx.doi.org/10.1007/978-1-62703-032-8_1
  2. ↑ Ishibashi T, Kimura S, Furukawa T, Hatanaka M, Hashimoto J, Sakaguchi K. Two types of replication protein A 70 kDa subunit in rice, Oryza sativa: molecular cloning, characterization, and cellular & tissue distribution. Gene. 2001 Jul 11;272(1-2):335-43. PMID:11470540 doi:10.1016/s0378-1119(01)00555-8

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Michal Harel, Alexander Berchansky

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