Ubiquitin protein ligase

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:35, 22 August 2024) (edit) (undo)
 
(67 intermediate revisions not shown.)
Line 1: Line 1:
-
+
<StructureSection load='' size='350' side='right' caption='Human UPL HOIP (magenta) complex with ubiquitin (green), imidazole and Zn+2 ion (grey) [[4ljo]]' scene='53/537519/Cv/1' >
-
[[Image:1c4z.png|left|200px|thumb|Crystal structure of Human UPL catalytic domain trimer (grey, green, pink) complex with ubiquitin conjugating enzyme E2 (yellow), [1c4z]]]
+
== Function ==
-
{{STRUCTURE_1c4z| PDB=1c4z | SIZE=400| SCENE= |right|CAPTION=Human UPL catalytic domain trimer (grey, green, pink) complex with ubiquitin conjugating enzyme E2 (yellow) [[1c4z]] }}
+
'''Ubiquitin-protein ligase''' (UPL) ('''E3''') or '''E3 ubiquitin-protein ligase''' in combination with ubiquitin-conjugating enzyme (E2) causes the attachment of ubiquitin to lysine in a target protein<ref>PMID:16250895</ref>.
 +
*'''UPL XIAP''' or '''X-linked Inhibitor of Apoptosis Protein''' stops apoptosis induced by viral infection or by overproduction of caspases. UPL XIAP binds caspase-3, -7 and -9<ref>PMID:17549347</ref>. UPL XIAP is inhibited by Smac – a death signaling protein.
 +
*'''RING-type E3 ubiquitin transferase''' are E3s which do not have Zn+2 coordination<ref>PMID:23747565</ref>.
 +
*'''HOIL-1''' or '''RanBP-type and C3HC4-type zinc finger-containing protein 1''' is an atypical E3 ligase which forms ester bonds between ubiquitin and Ser/Thr residues in proteins<ref>PMID:33932090</ref>.
 +
*'''NEDD4-like Ubiquitin protein ligase WWP''' see [[WWP2]].
 +
Examples of E3 ubiquitin-protein ligases include [[Parkin]]
-
+
== Disease ==
 +
XIAP mutations cause X-linked lymphoproliferative syndrome type 2<ref>PMID:24942515</ref>. Parkin UPL has a direct causal role in neurodegenerative disorders<ref>PMID:16908979</ref>. Cereblon nonsense mutation causes autosomal recessive nonsyndromic mental retardation<ref>PMID:23983124</ref>.
 +
== Relevance ==
 +
Overexpression of UPL XIAP functions as tumor marker. UPL '''cereblon''' is inhibited by thalidomide<ref>PMID:24292623</ref> and [[Lenalidomide]].
 +
== Structural highlights ==
 +
UPL can contain several distinct domains like:<br />
 +
* '''WW''' domains which contain 2 tryptophans which bind proline-rich peptide;<br />
 +
* '''UBA''' – Ubiquitin-Associated Domain;<br />
 +
* '''TKB''' – Tyrosine Kinase Binding domain;<br />
 +
* '''C2''' – an 8 β strand domain which coordinates targeting proteins toward cell membrane;<br />
 +
* '''Cereblon''' is a target for thalidomide;<br />
 +
* '''HECT''' – Homologous to the E6-AP C-terminus;<br />
 +
* '''RING''' – Really Intersting New Gene involved in binding to E2;<br />
 +
* '''FHA''' – Forkhead Associated domain recognizing phosphothreonine;<br />
 +
* '''IBR''' – involved in binding to E2;<br />
 +
* '''PHD''' finger – Plant Homeo Domain is 50-80 amino acid long which contains a CHC motif;<br />
 +
* '''SRA – SET and RING-Associated''' domain;<br />
 +
* '''TUDOR''' – a ca. 50 amino acids long and recognizes dimethylated arginine;<br />
 +
* '''CPH''' – protein-protein interaction domain;<br />
 +
* '''UBR''' – a ca. 70 amino acid long zinc finger-like domain;<br />
 +
* '''CUE''' – a ubiquitin-binding domain.<br />
 +
'''UPL XIAP''' consists of 3 BIR (baculoviral IAP repeat) domains, followed by UBA (ubiquitin-binding) domain and RING (C-terminal RING finger) domain. BIR2 inhibits caspase-3 and -7. BIR3 inhibits caspase-9.
 +
UPL contains an thioester-forming cysteine which catalyzes the transfer of ubiquitin from E2 to a substrate lysine. The surface interacting residues of UPL and the acceptor ubiquitin are shown here<ref>PMID:24141947</ref>.
-
 
+
*<scene name='53/537519/Cv/8'>Ubiquitin/protein ligase interface shown in space-filled representation</scene>.
-
 
+
*<scene name='53/537519/Cv/10'>Ubiquitin/protein ligase interface interactions</scene>.
-
 
+
*<scene name='53/537519/Cv/9'>1st Zn coordination site</scene>.
-
 
+
*<scene name='53/537519/Cv/11'>2nd Zn coordination site</scene>.
-
 
+
*<scene name='53/537519/Cv/12'>3rd Zn coordination site</scene>.
-
 
+
*<scene name='53/537519/Cv/13'>4th Zn coordination site</scene>.
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
'''Ubiquitin-protein ligase''' (UPL) ('''E3''')in combination with ubiquitin-conjugating enzyme (E2) causes the attachment of ubiquitin to lysine to a target protein. '''UPL XIAP''' or '''X-linked Inhibitor of Apoptosis Protein''' stops apoptosis induced by viral infection or by overproduction of caspases. UPL XIAP or '''baculoviral IAP repeat-containing protein''', binds caspase-3, -7 and -9. UPL can contain several distinct domains like: WW domains which contains 2 tryptophans which bind proline-rich peptide; UBA – Ubiquitin-Associated Domain; TKB – Tyrosine Kinase Binding domain; C2 – an 8 β strand domain which coordinates targeting proteins toward cell membrane; HECT – Homologous to the E6-AP Carboxyl Terminus; RING – Really Intersting New Gene involved in binding to E2; FHA – Forkhead Associated domain recognizing phosphothreonine; IBR – involved in binding to E2; PHD finger – Plant Homeo Domain is 50-80 amino acid long which contains a CHC motif; SRA – SET and RING-Associated domain; TUDOR – is a ca. 50 amino acids long and recognizes dimethylated arginine; CPH – protein-protein interaction domain; UBR – a ca. 70 amino acid long zinc finger-like domain; CUE – a ubiquitin-binding domain. UPL XIAP consists of 3 BIR (baculoviral IAP repeat) domains, followed by UBA (ubiquitin-binding) domain and RING (C-terminal RING finger) domain. BIR2 inhibits caspase-3 and -7. BIR3 inhibits caspase-9. Overexpression of UPL XIAP functions as tumor marker. UPL XIAP is inhibited by Smac – a death signaling protein.
+
For some details see<br />
For some details see<br />
*[[TIR1 ubiquitin ligase]]<br />
*[[TIR1 ubiquitin ligase]]<br />
*[[Herbicide 2,4-D bound to TIR1 Ubiquitin Ligase]].
*[[Herbicide 2,4-D bound to TIR1 Ubiquitin Ligase]].
- 
== 3D Structures of ubiquitin protein ligase==
== 3D Structures of ubiquitin protein ligase==
-
+
[[Ubiquitin protein ligase 3D structures]]
-
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+
-
 
+
-
===UPL AMFR===
+
-
 
+
-
[[4g3o]] – hUPL CUE domain<br />
+
-
[[2lvn]] – hUPL CUE domain - NMR<br />
+
-
[[2lxh]] – hUPL RING domain - NMR<br />
+
-
[[2lvo]], [[2lvp]], [[2lvq]] – hUPL CUE domain + ubiquitin - NMR<br />
+
-
[[2lxp]] – hUPL RING+G2BR domains + E2 G2 - NMR<br />
+
-
[[4lad]] – hUPL RING domain (mutant) + E2 G2 <br />
+
-
 
+
-
===UPL Arkadia===
+
-
 
+
-
[[2kiz]] – hUPL RING-H2 finger domain - NMR<br />
+
-
 
+
-
UPL CHFR
+
-
 
+
-
[[2xoc]], [[2xoy]], [[2xoz]] – hUPL C terminal + nucleotide<br />
+
-
[[2xp0]] – hUPL C terminal <br />
+
-
 
+
-
===UPL Cbl===
+
-
 
+
-
[[2y1m]] – hUPL - human<br />
+
-
[[1d5f]] – hUPL catalytic domain <br />
+
-
[[2dk3]] – hUPL catalytic domain - NMR<br />
+
-
[[1yiu]] – hUPL WW3 domain - NMR<br />
+
-
[[2k4d]] – hUPL RING domain<br />
+
-
[[2oo9]], [[2ooa]], [[2oob]] – hUPL UBA domain <br />
+
-
[[2do6]], [[2ekk]], [[2jnh]], [[2juj]] – hUPL UBA domain - NMR<br />
+
-
[[3vgo]] – hUPL N terminal <br />
+
-
[[2kr1]] – hUPL N terminal - NMR <br />
+
-
[[2ldr]] – hUPL helix-ring domain - NMR <br />
+
-
[[2d9s]] – mUPL UBA domain – mouse - NMR<br />
+
-
 
+
-
''UPL Cbl complex''
+
-
 
+
-
[[1yvh]] – hUPL TKB domain + APS adaptor protein<br />
+
-
[[3bum]], [[3bun]], [[3buo]], [[3buw]], [[3bux]], [[3ob1]], [[3ob2]], [[3rlf]], [[3pfv]], [[2k4d]], [[4gpl]] – hUPL TKB domain + peptide <br />
+
-
[[2y1n]] – hUPL residues 47-435 + ZAP peptide <br />
+
-
[[4a4b]], [[4a4c]] – hUPL TKB domain (mutant) + ZAP peptide + ubiquitin conjugating enzyme E2 <br />
+
-
[[1c4z]] – hUPL catalytic domain + ubiquitin conjugating enzyme E2 <br />
+
-
[[4a49]] – hUPL residues 354-435 + ubiquitin conjugating enzyme E2 <br />
+
-
 
+
-
===UPL CNOT4===
+
-
 
+
-
[[2cpi]] – mUPL RNA recognition motif - NMR <br />
+
-
 
+
-
===UPL DOA10===
+
-
 
+
-
[[2m6m]] – hUPL RING domain - NMR<br />
+
-
 
+
-
===UPL DTX3L===
+
-
 
+
-
[[3pg6]] – hUPL C terminal <br />
+
-
 
+
-
===UPL E3===
+
-
 
+
-
[[2y1m]] – hUPL - human<br />
+
-
[[1d5f]] – hUPL catalytic domain <br />
+
-
[[2dk3]] – hUPL catalytic domain - NMR<br />
+
-
[[1yiu]] – hUPL WW3 domain - NMR<br />
+
-
[[2oo9]], [[2ooa]], [[2oob]] – hUPL UBA domain <br />
+
-
[[2do6]], [[2ekk]], [[2jnh]], [[2juj]] – hUPL UBA domain - NMR<br />
+
-
[[3vgo]] – hUPL N terminal <br />
+
-
[[2kr1]] – hUPL N terminal - NMR <br />
+
-
[[2ldr]] – hUPL helix-ring domain - NMR <br />
+
-
[[2d9s]] – mUPL UBA domain – mouse - NMR<br />
+
-
 
+
-
''UPL E3 complex''
+
-
 
+
-
[[1yvh]] – hUPL TKB domain + APS adaptor protein<br />
+
-
[[3bum]], [[3bun]], [[3buo]], [[3buw]], [[3bux]], [[3ob1]], [[3ob2]], [[3rlf]], [[3pfv]] – hUPL TKB domain + peptide <br />
+
-
[[2y1n]] – hUPL residues 47-435 + ZAP peptide <br />
+
-
[[4a4b]], [[4a4c]] – hUPL TKB domain (mutant) + ZAP peptide + ubiquitin conjugating enzyme E2 <br />
+
-
[[1c4z]] – hUPL catalytic domain + ubiquitin conjugating enzyme E2 <br />
+
-
[[4a49]] – hUPL residues 354-435 + ubiquitin conjugating enzyme E2 <br />
+
-
 
+
-
===UPL EDD1===
+
-
 
+
-
[[2qho]] – hUPL UBA domain + ubiquitin <br />
+
-
 
+
-
===UPL FANCL===
+
-
 
+
-
[[3zqs]] – hUPL residues 109-294<br />
+
-
[[4ccg]] – hUPL RING domain + E2 T<br />
+
-
 
+
-
===UPL HAKAI===
+
-
 
+
-
[[3vk6]] – mUPL phosphotyrosine-binding domain<br />
+
-
 
+
-
===UPL HECTD1===
+
-
 
+
-
[[3dkm]] – hUPL CPH domain <br />
+
-
[[2lc3]] – hUPL helical bundle domain - NMR<br />
+
-
 
+
-
===UPL HECW===
+
-
 
+
-
[[3l4h]] – hUPL WW2 and helical box domains <br />
+
-
[[2lfe]] – hUPL N terminal - NMR<br />
+
-
 
+
-
===UPL HERC===
+
-
 
+
-
[[4o2w]] – hUPL 1 3rd RCC1-like domain <br />
+
-
[[4l1m]] – hUPL 2 1st RCC1-like domain <br />
+
-
 
+
-
===UPL HHARI===
+
-
 
+
-
[[4kbl]], [[4kc9]] – hUPL<br />
+
-
[[2m9y]] – hUPL catalytic domain - NMR<br />
+
-
 
+
-
===UPL HUWE1===
+
-
 
+
-
[[3g1n]] – hUPL catalytic domain <br />
+
-
[[3h1d]] – hUPL HECT domain (mutant)<br />
+
-
 
+
-
===UPL ITCHY===
+
-
 
+
-
[[2jo9]] – mUPL WW3 domain + Epstein-Barr virus peptide - NMR <br />
+
-
[[2joc]], [[2ysf]], [[2kyk]] – mUPL WW domain - NMR <br />
+
-
[[3tug]] – hUPL HECT domain <br />
+
-
[[2nq3]] – hUPL C2 domain <br />
+
-
[[2dmv]] – mUPL WW2 domain - NMR <br />
+
-
 
+
-
===UPL LNX===
+
-
 
+
-
[[3vqf]] – mUPL 2rd PDZ domain <br />
+
-
[[3b76]] – hUPL 3rd PDZ domain <br />
+
-
[[3vqg]] – mUPL 2rd PDZ domain + peptide<br />
+
-
 
+
-
===UPL MDM2===
+
-
 
+
-
[[MDM2]]
+
-
 
+
-
===UPL MYCBP2===
+
-
 
+
-
[[3gbw]] – mUPL 1st PHR domain <br />
+
-
[[3hwj]] – mUPL 2nd PHR domain <br />
+
-
 
+
-
===UPL MYLIP===
+
-
 
+
-
[[2yhn]] – hUPL RING domain <br />
+
-
[[2yho]] – hUPL RING domain + ubiquitin-conjugating enzyme<br />
+
-
 
+
-
===UPL NEDD4===
+
-
 
+
-
[[1wr3]], [[1wr4]], [[1wr7]] – mUPL WW domain - NMR <br />
+
-
[[2oni]] – hUPL catalytic domain <br />
+
-
[[3b7y]] – hUPL C2 domain <br />
+
-
[[2xbf]] – hUPL HECT domain <br />
+
-
[4be8]] – hUPL HECT domain (mutant) <br />
+
-
 
+
-
''UPL NEDD4 complex''
+
-
 
+
-
[[1i5h]] – rUPL WW3 domain + amiloride-sensitive Na channel β subunit – rat <br />
+
-
[[2ltx]], [[2lty]] – hUPL WW2 domain + peptide - NMR <br />
+
-
[[2ez5]] – UPL WW3 domain + COMM LPSY peptide – Drosophila melanogaster - NMR <br />
+
-
[[2kpz]], [[2kq0]], [[2laj]], [[2ltz]], [[2m3o]] – hUPL WW3 domain + peptide - NMR <br />
+
-
[[4n7h]] – hUPL WW3 domain + arrestin peptide <br />
+
-
[[2lb2]] – hUPL 2nd domain + peptide - NMR <br />
+
-
[[3m7f]] – hUPL C2 domain + growth factor receptor bound protein<br />
+
-
[[3jvz]], [[3jw0]] – hUPL HECT domain + ubiquitin + ubiquitin-conjugating enzyme<br />
+
-
[[2xbb]] – hUPL HECT domain + ubiquitin <br />
+
-
[[4bbn]] – hUPL HECT domain (mutant) + polyubiquitin <br />
+
-
 
+
-
===UPL Praja===
+
-
 
+
-
[[2l0b]] – hUPL RING-type zinc finger domain - NMR<br />
+
-
 
+
-
===UPL PARKIN===
+
-
 
+
-
[[4k95]] – rUPL <br />
+
-
[[2jmo]] – hUPL IBR domain - NMR <br />
+
-
[[2zeq]] – hUPL ubiquitin-like domain <br />
+
-
[[4bm9]], [[4i1h]] – hUPL catalytic domain <br />
+
-
[[4i1f]] – hUPL catalytic domain (mutant)<br />
+
-
[[3b1l]] – mUPL ubiquitin-like domain <br />
+
-
[[4k7d]] – rUPL C terminal domain <br />
+
-
[[2knb]] – rUPL + endophilin - NMR <br />
+
-
 
+
-
===UPL RAD18===
+
-
 
+
-
[[2y43]] – hUPL RING domain <br />
+
-
 
+
-
===UPL RBBP6===
+
-
 
+
-
[[3ztg]] – hUPL RING domain - NMR <br />
+
-
 
+
-
===UPL RBX===
+
-
 
+
-
[[2lgv]] – hUPL residues 5-108 (mutant) - NMR <br />
+
-
[[3rtr]], [[4f52]] – hUPL residues 5-108 + cullin-1 <br />
+
-
[[4a0k]], [[4a0c]] – hUPL residues 12-108 + cullin-4B + cullin-associated NEDD8-dissociated protein<br />
+
-
[[4a0l]] – hUPL residues 12-108 + cullin-4B + DNA damage-binding protein + DNA<br />
+
-
 
+
-
===UPL RING2===
+
-
 
+
-
[[2ckl]] – mUPL RING domain + polycomb group ring finger protein 4 <br />
+
-
[[2h0d]] – mUPL RING domain + B lymphoma MO-MLV insertion region <br />
+
-
[[3h8h]] – hUPL C terminal <br />
+
-
[[3gs2]] – hUPL C terminal + chromobox protein homolog<br />
+
-
[[3ixs]] – hUPL C terminal (mutant) + RING1 and YY1-binding protein<br />
+
-
[[3rpg]] – hUPL + polycomb complex protein BMI-1 + E2 D3 <br />
+
-
 
+
-
===UPL RNF===
+
-
 
+
-
[[4ppe]] – hUPL RING domain <br />
+
-
[[4ap4]] – hUPL 4 residues 131-195 + E2 + ubiquitin<br />
+
-
[[2pie]] – hUPL 8 FHA domain <br />
+
-
[[2csw]] – hUPL 8 FHA domain - NMR <br />
+
-
[[4ayc]], [[4gb0]] – hUPL 8 RING domain <br />
+
-
[[4epo]], [[4orh]] – hUPL 8 residues 345-485 + E2 <br />
+
-
[[4juy]], [[4oyj]], [[4p09]] – hUPL 31 PUB domain <br />
+
-
[[4ljq]] – hUPL 31 catalytic domain <br />
+
-
[[4ljo]] – hUPL 31 catalytic domain + ubiquitin<br />
+
-
[[4ljp]] – hUPL 31 catalytic domain (mutant) + ubiquitin<br />
+
-
[[4owf]] – hUPL 31 catalytic domain + nf-κ-B modulator<br />
+
-
[[4oyk]], [[4p0b]] – hUPL 31 PUB domain + otulin<br />
+
-
[[4p0a]] – hUPL 31 PUB domain + p97 peptide<br />
+
-
[[4kng]] – hUPL 43 PA domain + LGR5 + RSPO1<br />
+
-
[[2ma6]] – hUPL 123 RING domain - NMR<br />
+
-
[[3icu]] – hUPL 128 protease-associated domain <br />
+
-
[[3v3l]] – hUPL 146 WWE domain <br />
+
-
[[2rsf]] – mUPL 146 WWE domain + ATP<br />
+
-
[[3l11]], [[4gb0]] – hUPL 168 RING domain <br />
+
-
 
+
-
===UPL RSP===
+
-
 
+
-
[[4lcd]] – hUPL 3 HECT + WW3 domains (mutant) + SNA3 peptide + ubiquitin <br />
+
-
[[3olm]] – hUPL 5 HECT + WW3 domains + ubiquitin <br />
+
-
 
+
-
===UPL SHPRH===
+
-
 
+
-
[[2m85]] – hUPL PHD domain - NMR<br />
+
-
 
+
-
===UPL SIAH1===
+
-
 
+
-
[[4c9z]], [[4ca1]] – hUPL zinc finger+substrate binding domains <br />
+
-
[[2a25]] – hUPL substrate binding domain + calcyclin-binding protein peptide <br />
+
-
[[4i7b]], [[4i7d]] – hUPL C terminal + phyllopod peptide <br />
+
-
[[4i7c]] – hUPL C terminal (mutant) + phyllopod peptide <br />
+
-
[[2an6]] – mUPL residues 92-282 + phyllopod peptide <br />
+
-
 
+
-
===UPL SMURF===
+
-
 
+
-
[[2jqz]] – hUPL - NMR <br />
+
-
[[2kxq]] – hUPL WW2 and WW3 + peptide<br />
+
-
[[2laz]], [[2lb0]], [[2lb1]], [[2ltx]] – hUPL WW + peptide - NMR<br />
+
-
[[3pyc]] – hUPL C2 domain <br />
+
-
 
+
-
===UPL SOPA===
+
-
 
+
-
[[3sy2]] – SeUPL residues 165-782 + ubiquitin + ubiquitin-conjugating enzyme – ''Salmonella enterica''<br />
+
-
 
+
-
===UPL SSPH1===
+
-
 
+
-
[[4nkh]] –SeUPL 21 LRR domain <br />
+
-
[[4nkg]] –SeUPL 21 LRR domain + Ser/Thr protein kinase N1 peptide<br />
+
-
 
+
-
===UPL TIR1===
+
-
 
+
-
[[2p1q]] – AtUPL + auxin response protein + SKP1-like protein – ''Arabidopsis thaliana''<br />
+
-
[[2p1q]] – AtUPL + auxin response protein + SKP1-like protein + herbicide<br />
+
-
 
+
-
===UPL TRIM===
+
-
 
+
-
[[3zo0]] – mUPL 21 residues 291-470 + IG γ-2A<br />
+
-
[[4b8e]] – mUPL 25 PRY-SPRY domain <br />
+
-
[[3u5m]] – hUPL 33 PHD+Bromo domains <br />
+
-
[[3u5n]], [[3u5o]], [[3u5p]] – hUPL 33 PHD+Bromo domain + H3-histone peptide <br />
+
-
[[3ddt]] – hUPL 37 B2-box <br />
+
-
[[4m3l]] – hUPL 63 coiled-coil domain <br />
+
-
[[4nqj]] – hUPL 69 coiled-coil domain <br />
+
-
 
+
-
===UPL UBR===
+
-
 
+
-
[[3ny1]], [[3ny2]] – hUPL UBR-box <br />
+
-
[[3pt3]] – hUPL C terminal <br />
+
-
[[3ny3]] – hUPL UBR-box + N-degron<br />
+
-
[[3nih]], [[3nii]], [[3nij]], [[3nik]], [[3nil]], [[3nim]], [[3nin]] – yUPL UBR-box + peptide - yeast<br />
+
-
[[3nis]], [[3nit]] – yUPL UBR-box <br />
+
-
[[3ntw]] – rUPL MLLE domain + peptide <br />
+
-
 
+
-
===UPL UHRF (Np95)===
+
-
 
+
-
[[3sox]], [[3zvz]] – hUPL 1 PHD finger <br />
+
-
[[2lgl]] – hUPL 1 PHD finger - NMR <br />
+
-
[[2e6s]] – hUPL 2 PHD finger - NMR <br />
+
-
[[3zvy]] – hUPL 1 PHD finger + histone H3 peptide<br />
+
-
[[3bi7]], [[2pb7]], [[2zkg]], [[3dwh]] – hUPL 1 SRA domain <br />
+
-
[[3oln]] – hUPL 2 SRA domain <br />
+
-
[[3db4]] – hUPL 1 TUDOR domain <br />
+
-
[[3fl2]] – hUPL 1 RING domain <br />
+
-
 
+
-
''UPL UHRF complex''
+
-
 
+
-
[[3clz]], [[2zkd]], [[2zke]], [[2zkf]] – hUPL 1 SRA domain + DNA<br />
+
-
[[4pw5]], [[4pw6]], [[4pw7]] – hUPL 2 SRA domain + DNA<br />
+
-
[[2zo0]], [[2zo1]], [[2zo2]], [[3f8i]], [[3f8j]], [[3fde]] – mUPL 1 SRA domain + DNA<br />
+
-
[[3sou]], [[3sow]], [[3shb]], [[2lgg]], [[3t6r]], [[3ask]], [[3asl]], [[4gy5]] – hUPL 1 PHD finger + histone H3 peptide<br />
+
-
[[2lgk]] – hUPL 1 PHD finger + histone H3 peptide - NMR<br />
+
-
[[3db3]] – hUPL 1 TUDOR domain + histone H3 peptide<br />
+
-
[[2l3r]] – hUPL 1 TUDOR domain + histone H3 peptide - NMR<br />
+
-
 
+
-
===UPL WWP1===
+
-
 
+
-
[[2jqz]] – hUPL WW4 domain - NMR <br />
+
-
 
+
-
===UPL XIAP===
+
-
 
+
-
''XIAP BIR-1 domain''
+
-
 
+
-
[[2poi]], [[2qra]] – hXIAP – human <BR />
+
-
[[2pop]] – hXIAP + TAB1 <BR />
+
-
 
+
-
''XIAP BIR-2 domain''
+
-
 
+
-
[[1c9q]] – hXIAP (mutant) – NMR <BR />
+
-
[[4j3y]] – hXIAP (mutant) <BR />
+
-
[[4j44]], [[4j45]], [[4j46]], [[4j47]], [[4j48]] - hXIAP (mutant) + peptide<BR />
+
-
[[1i3o]] – hXIAP (mutant) + caspase-3 <BR />
+
-
[[1i4o]], [[1kmc]], [[1i51]] – hXIAP + caspase-7 <BR />
+
-
[[4kmp]] - hXIAP + inhibitor<BR />
+
-
[[4kju]], [[4kjv]] - hXIAP (mutant) + inhibitor<BR />
+
-
 
+
-
[[3uw5]] – hXIAP residues 63-179 (mutant) + GDC0152 + inhibitor<br /> <BR />
+
-
 
+
-
''XIAP BIR-3 domain''
+
-
 
+
-
[[1f9x]] – hXIAP – NMR <BR />
+
-
[[1g3f]] – hXIAP + Smac peptide – NMR <BR />
+
-
[[2opz]] – hXIAP + Smac peptide <BR />
+
-
[[2opy]], [[2vsl]], [[3clx]], [[3cm2]], [[3cm7]], [[2jk7]], [[3eyl]], [[3g76]], [[4ec4]] – hXIAP + Smac mimic <BR />
+
-
[[1g73]] – hXIAP + Smac <BR />
+
-
[[1nw9]] – hXIAP + caspase-9 <BR />
+
-
[[1tfq]], [[1tft]] – hXIAP + inhibitor - NMR <BR />
+
-
[[3hl5]], [[4hy0]] – hXIAP + inhibitor <BR />
+
-
[[3uw4]] – hXIAP (mutant) + peptide + inhibitor <BR />
+
-
 
+
-
''XIAP UBA domain''
+
-
 
+
-
[[2kna]] – hXIAP – NMR <BR />
+
-
 
+
-
''XIAP RING domain''
+
-
 
+
-
[[4ic2]] – hXIAP <BR />
+
-
[[2ecg]] – hXIAP – NMR <BR />
+
-
[[4ic3]] – hXIAP (mutant) <BR />
+
-
 
+
-
 
+
-
===UPL ZNRF3===
+
-
 
+
-
[[4c84]], [[4c85]], [[4c86]], [[4c8a]], [[4c8c]], [[4c8f]] – zUPL ectodomain – zebra fish <br />
+
-
[[4c8p]] – zUPL ectodomain (mutant) <br />
+
-
[[4c8t]], [[4c8u]] – fUPL ectodomain – frog <br />
+
-
[[4cdj]] – mUPL ectodomain <br />
+
-
[[4c99]], [[4c9a]], [[4c9e]], [[4c9r]], [[4c9u]], [[4cdk]] – mUPL ectodomain + R-spondin <br />
+
-
 
+
-
===UPL ZFP91===
+
-
 
+
-
[[2m9a]] – hUPL residues 370-456 - NMR<br />
+
-
 
+
-
 
+
-
+
-
 
+
-
 
+
-
 
+
-
 
+
 +
</StructureSection>
-
[[Category:Topic Page]]
+
==References==
 +
<references />
 +
[[Category:Topic Page]] + phosphoubiquitin

Current revision

Human UPL HOIP (magenta) complex with ubiquitin (green), imidazole and Zn+2 ion (grey) 4ljo

Drag the structure with the mouse to rotate

References

  1. ↑ Ardley HC, Robinson PA. E3 ubiquitin ligases. Essays Biochem. 2005;41:15-30. PMID:16250895 doi:http://dx.doi.org/10.1042/EB0410015
  2. ↑ Sakemi R, Yano H, Ogasawara S, Akiba J, Nakashima O, Fukahori S, Sata M, Kojiro M. X-linked inhibitor of apoptosis (XIAP) and XIAP-associated factor-1 expressions and their relationship to apoptosis in human hepatocellular carcinoma and non-cancerous liver tissues. Oncol Rep. 2007 Jul;18(1):65-70. PMID:17549347
  3. ↑ Metzger MB, Pruneda JN, Klevit RE, Weissman AM. RING-type E3 ligases: master manipulators of E2 ubiquitin-conjugating enzymes and ubiquitination. Biochim Biophys Acta. 2014 Jan;1843(1):47-60. PMID:23747565 doi:10.1016/j.bbamcr.2013.05.026
  4. ↑ Petrova T, Zhang J, Nanda SK, Figueras-Vadillo C, Cohen P. HOIL-1-catalysed, ester-linked ubiquitylation restricts IL-18 signaling in cytotoxic T cells but promotes TLR signalling in macrophages. FEBS J. 2021 Oct;288(20):5909-5924. PMID:33932090 doi:10.1111/febs.15896
  5. ↑ Aguilar C, Lenoir C, Lambert N, Begue B, Brousse N, Canioni D, Berrebi D, Roy M, Gerart S, Chapel H, Schwerd T, Siproudhis L, Schappi M, Al-Ahmari A, Mori M, Yamaide A, Galicier L, Neven B, Routes J, Uhlig HH, Koletzko S, Patel S, Kanegane H, Picard C, Fischer A, Bensussan NC, Ruemmele F, Hugot JP, Latour S. Characterization of Crohn disease in X-linked inhibitor of apoptosis-deficient male patients and female symptomatic carriers. J Allergy Clin Immunol. 2014 Nov;134(5):1131-41.e9. doi:, 10.1016/j.jaci.2014.04.031. Epub 2014 Jun 15. PMID:24942515 doi:http://dx.doi.org/10.1016/j.jaci.2014.04.031
  6. ↑ Ardley HC, Robinson PA. The role of ubiquitin-protein ligases in neurodegenerative disease. Neurodegener Dis. 2004;1(2-3):71-87. PMID:16908979 doi:http://dx.doi.org/10.1159/000080048
  7. ↑ Xu G, Jiang X, Jaffrey SR. A mental retardation-linked nonsense mutation in cereblon is rescued by proteasome inhibition. J Biol Chem. 2013 Oct 11;288(41):29573-85. doi: 10.1074/jbc.M113.472092. Epub, 2013 Aug 27. PMID:23983124 doi:http://dx.doi.org/10.1074/jbc.M113.472092
  8. ↑ Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK, Bradner JE, Kaelin WG Jr. The myeloma drug lenalidomide promotes the cereblon-dependent destruction of Ikaros proteins. Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. Epub 2013 Nov, 29. PMID:24292623 doi:http://dx.doi.org/10.1126/science.1244917
  9. ↑ Stieglitz B, Rana RR, Koliopoulos MG, Morris-Davies AC, Schaeffer V, Christodoulou E, Howell S, Brown NR, Dikic I, Rittinger K. Structural basis for ligase-specific conjugation of linear ubiquitin chains by HOIP. Nature. 2013 Oct 20. doi: 10.1038/nature12638. PMID:24141947 doi:http://dx.doi.org/10.1038/nature12638
+ phosphoubiquitin
Retrieved from "http://52.214.119.220/wiki/index.php/Ubiquitin_protein_ligase"
Personal tools