1xqm
From Proteopedia
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(New page: 200px<br /><applet load="1xqm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xqm, resolution 2.1Å" /> '''Variations on the GFP...) |
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| - | [[Image:1xqm.jpg|left|200px]]<br /><applet load="1xqm" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1xqm, resolution 2.1Å" /> | ||
| - | '''Variations on the GFP chromophore scaffold: A fragmented 5-membered heterocycle revealed in the 2.1A crystal structure of a non-fluorescent chromoprotein'''<br /> | ||
| - | == | + | ==Variations on the GFP chromophore scaffold: A fragmented 5-membered heterocycle revealed in the 2.1A crystal structure of a non-fluorescent chromoprotein== |
| - | We have determined to 2.1 A resolution the crystal structure of a dark | + | <StructureSection load='1xqm' size='340' side='right'caption='[[1xqm]], [[Resolution|resolution]] 2.10Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1xqm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Anemonia_sulcata Anemonia sulcata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XQM FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CH6:{(4Z)-2-[(1S)-1-AMINO-3-(METHYLSULFANYL)PROPYL]-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CH6</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xqm OCA], [https://pdbe.org/1xqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xqm RCSB], [https://www.ebi.ac.uk/pdbsum/1xqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xqm ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/NFCP_ANESU NFCP_ANESU] Pigment protein that is intensely purple in color.<ref>PMID:10852900</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xq/1xqm_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xqm ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We have determined to 2.1 A resolution the crystal structure of a dark state, kindling fluorescent protein isolated from the sea anemone, Anemonia sulcata. The chromophore sequence Met(63)-Tyr(64)-Gly(65) of the A. sulcata chromoprotein was previously proposed to comprise a 6-membered pyrazine-type heterocycle (Martynov, V. I., Savitsky, A. P., Martynova, N. Y., Savitsky, P. A., Lukyanov, K. A., and Lukyanov, S. A. (2001) J. Biol. Chem. 276, 21012-21016). However, our crystallographic data revealed the chromophore to comprise a 5-membered p-hydroxybenzylideneimidazolinone moiety that adopts a non-coplanar trans conformation within the interior of the GFP beta-can fold. Unexpectedly, fragmentation of the polypeptide was found to occur within the chromophore moiety, at the bond between Cys(62C) and Met(63N1.) Our structural data reveal that fragmentation of the chromophore represents an intrinsic, autocatalytic step toward the formation of the mature chromophore within the specific GFP-like proteins. | ||
| - | + | Variations on the GFP chromophore: A polypeptide fragmentation within the chromophore revealed in the 2.1-A crystal structure of a nonfluorescent chromoprotein from Anemonia sulcata.,Wilmann PG, Petersen J, Devenish RJ, Prescott M, Rossjohn J J Biol Chem. 2005 Jan 28;280(4):2401-4. Epub 2004 Nov 12. PMID:15542608<ref>PMID:15542608</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 1xqm" style="background-color:#fffaf0;"></div> |
| - | [[Category: Devenish | + | == References == |
| - | [[Category: Petersen | + | <references/> |
| - | [[Category: Prescott | + | __TOC__ |
| - | [[Category: Rossjohn | + | </StructureSection> |
| - | [[Category: Wilmann | + | [[Category: Anemonia sulcata]] |
| - | + | [[Category: Large Structures]] | |
| - | + | [[Category: Devenish RJ]] | |
| - | + | [[Category: Petersen J]] | |
| - | + | [[Category: Prescott M]] | |
| + | [[Category: Rossjohn J]] | ||
| + | [[Category: Wilmann PG]] | ||
Current revision
Variations on the GFP chromophore scaffold: A fragmented 5-membered heterocycle revealed in the 2.1A crystal structure of a non-fluorescent chromoprotein
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