2cj4

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{{Seed}}
 
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[[Image:2cj4.png|left|200px]]
 
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==Crystal Structure of a Cell Wall Invertase Inhibitor from Tobacco at pH 4.6==
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The line below this paragraph, containing "STRUCTURE_2cj4", creates the "Structure Box" on the page.
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<StructureSection load='2cj4' size='340' side='right'caption='[[2cj4]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2cj4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CJ4 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2cj4| PDB=2cj4 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cj4 OCA], [https://pdbe.org/2cj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cj4 RCSB], [https://www.ebi.ac.uk/pdbsum/2cj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cj4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O49908_TOBAC O49908_TOBAC]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cj/2cj4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cj4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Plant acid invertases catalyse the breakdown of sucrose. Their activity is tightly regulated through interaction with specific protein inhibitors. The complex between the cell-wall invertase inhibitor Nt-CIF and its target enzyme is stable only at acidic pH, as found in the plant cell wall. Since the pH in this compartment can be modulated between pH 4 and 6 in planta, the rapid dissociation of the inhibitor-enzyme complex at neutral pH may represent a regulatory event. Here, it is analyzed whether the inhibitory component undergoes structural rearrangements upon changes in the pH environment. Six crystal forms grown at pH 4.6-9.5 and diffracting up to 1.63 Angstrom indicate only small structural changes in CIF. This suggests that complex dissociation at neutral pH is mediated either by rearrangements in the enzyme or by a complex pattern of surface charges in the inhibitor-enzyme binding interface.
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===CRYSTAL STRUCTURE OF A CELL WALL INVERTASE INHIBITOR FROM TOBACCO AT PH 4.6===
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Multiple crystal forms of the cell-wall invertase inhibitor from tobacco support high conformational rigidity over a broad pH range.,Hothorn M, Scheffzek K Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):665-70. Epub 2006, May 12. PMID:16699194<ref>PMID:16699194</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16699194}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2cj4" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16699194 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16699194}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2CJ4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJ4 OCA].
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==Reference==
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Multiple crystal forms of the cell-wall invertase inhibitor from tobacco support high conformational rigidity over a broad pH range., Hothorn M, Scheffzek K, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):665-70. Epub 2006, May 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16699194 16699194]
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[[Category: Nicotiana tabacum]]
[[Category: Nicotiana tabacum]]
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[[Category: Single protein]]
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[[Category: Hothorn M]]
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[[Category: Hothorn, M.]]
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[[Category: Scheffzek K]]
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[[Category: Scheffzek, K.]]
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[[Category: Four-helix bundle]]
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[[Category: Helical hairpin]]
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[[Category: Inhibitor]]
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[[Category: Protein binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:01:45 2008''
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Current revision

Crystal Structure of a Cell Wall Invertase Inhibitor from Tobacco at pH 4.6

PDB ID 2cj4

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