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| | ==NMR spatial structure of nonspecific lipid transfer protein from the dill Anethum graveolens L.== | | ==NMR spatial structure of nonspecific lipid transfer protein from the dill Anethum graveolens L.== |
| - | <StructureSection load='2n2z' size='340' side='right'caption='[[2n2z]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2n2z' size='340' side='right'caption='[[2n2z]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2n2z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Anegr Anegr]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N2Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2N2Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2n2z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Anethum_graveolens Anethum graveolens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N2Z FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2n2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n2z OCA], [http://pdbe.org/2n2z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n2z RCSB], [http://www.ebi.ac.uk/pdbsum/2n2z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n2z ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n2z OCA], [https://pdbe.org/2n2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n2z RCSB], [https://www.ebi.ac.uk/pdbsum/2n2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n2z ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/A0A0B4JDK1_ANEGR A0A0B4JDK1_ANEGR]] Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues.[RuleBase:RU000628] | + | [https://www.uniprot.org/uniprot/NLTP_ANEGR NLTP_ANEGR] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Anegr]] | + | [[Category: Anethum graveolens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Arseniev, A S]] | + | [[Category: Arseniev AS]] |
| - | [[Category: Finkina, E I]] | + | [[Category: Finkina EI]] |
| - | [[Category: Melnikova, D N]] | + | [[Category: Melnikova DN]] |
| - | [[Category: Mineev, K S]] | + | [[Category: Mineev KS]] |
| - | [[Category: Ovchinnikova, T V]] | + | [[Category: Ovchinnikova TV]] |
| - | [[Category: Lipid transfer protein]]
| + | |
| - | [[Category: Plant defense protein]]
| + | |
| - | [[Category: Plant protein]]
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| Structural highlights
Function
NLTP_ANEGR
Publication Abstract from PubMed
A novel lipid transfer protein, designated as Ag-LTP, was isolated from aerial parts of the dill Anethum graveolens L. Structural, antimicrobial, and lipid binding properties of the protein were studied. Complete amino acid sequence of Ag-LTP was determined. The protein has molecular mass of 9524.4 Da, consists of 93 amino acid residues including eight cysteines forming four disulfide bonds. The recombinant Ag-LTP was overexpressed in Escherichia coli and purified. NMR investigation shows that the Ag-LTP spatial structure contains four alpha-helices, forming the internal hydrophobic cavity, and a long C-terminal tail. The measured volume of the Ag-LTP hydrophobic cavity is equal to ~800 A(3) , which is much larger than those of other plant LTP1s. Ag-LTP has weak antifungal activity and unpronounced lipid binding specificity but effectively binds plant hormone jasmonic acid. Our results afford further molecular insight into biological functions of LTP in plants. Copyright (c) 2015 European Peptide Society and John Wiley & Sons, Ltd.
A novel lipid transfer protein from the dill Anethum graveolens L.: isolation, structure, heterologous expression, and functional characteristics.,Melnikova DN, Mineev KS, Finkina EI, Arseniev AS, Ovchinnikova TV J Pept Sci. 2016 Jan;22(1):59-66. doi: 10.1002/psc.2840. Epub 2015 Dec 17. PMID:26680443[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Melnikova DN, Mineev KS, Finkina EI, Arseniev AS, Ovchinnikova TV. A novel lipid transfer protein from the dill Anethum graveolens L.: isolation, structure, heterologous expression, and functional characteristics. J Pept Sci. 2016 Jan;22(1):59-66. doi: 10.1002/psc.2840. Epub 2015 Dec 17. PMID:26680443 doi:http://dx.doi.org/10.1002/psc.2840
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