3sfz

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[[Image:3sfz.jpg|left|200px]]
 
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==Crystal structure of full-length murine Apaf-1==
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The line below this paragraph, containing "STRUCTURE_3sfz", creates the "Structure Box" on the page.
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<StructureSection load='3sfz' size='340' side='right'caption='[[3sfz]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3sfz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. The September 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Apoptosomes'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_9 10.2210/rcsb_pdb/mom_2014_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SFZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GBL:GAMMA-BUTYROLACTONE'>GBL</scene></td></tr>
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{{STRUCTURE_3sfz| PDB=3sfz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sfz OCA], [https://pdbe.org/3sfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sfz RCSB], [https://www.ebi.ac.uk/pdbsum/3sfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sfz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/APAF_MOUSE APAF_MOUSE] Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The apoptotic protease-activating factor 1 (Apaf-1) relays the death signal in the mitochondrial pathway of apoptosis. Apaf-1 oligomerizes on binding of mitochondrially released cytochrome c into the heptameric apoptosome complex to ignite the downstream cascade of caspases. Here, we present the 3.0 A crystal structure of full-length murine Apaf-1 in the absence of cytochrome c. The structure shows how the mammalian death switch is kept in its "off" position. By comparing the off state with a recent cryo-electron microscopy derived model of Apaf-1 in its apoptosomal conformation, we depict the molecular events that transform Apaf-1 from autoinhibited monomer to a building block of the caspase-activating apoptosome. Moreover, we have solved the crystal structure of the R265S mutant of full-length murine Apaf-1 in the absence of cytochrome c to 3.55 A resolution and we show that proper function of Apaf-1 relies on R265 in the vicinity of the bound nucleotide.
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===Crystal structure of full-length murine Apaf-1===
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Crystal structure of full-length apaf-1: how the death signal is relayed in the mitochondrial pathway of apoptosis.,Reubold TF, Wohlgemuth S, Eschenburg S Structure. 2011 Aug 10;19(8):1074-83. PMID:21827944<ref>PMID:21827944</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3sfz" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Apoptotic protease-activating factor-1 3D structures|Apoptotic protease-activating factor-1 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21827944 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21827944}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Apoptosomes]]
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[[3sfz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SFZ OCA].
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[[Category: Large Structures]]
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==Reference==
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<ref group="xtra">PMID:021827944</ref><references group="xtra"/>
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Eschenburg, S.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Reubold, T F.]]
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[[Category: Eschenburg S]]
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[[Category: Adenine nucleotide]]
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[[Category: Reubold TF]]
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[[Category: Apoptosis]]
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[[Category: Caspase activation]]
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[[Category: Cytochrome c]]
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[[Category: Cytosol]]
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[[Category: Procaspase-9]]
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Current revision

Crystal structure of full-length murine Apaf-1

PDB ID 3sfz

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