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| | ==Crystal structure of an RGS-RhoGEF from Entamoeba histolytica== | | ==Crystal structure of an RGS-RhoGEF from Entamoeba histolytica== |
| - | <StructureSection load='4gou' size='340' side='right' caption='[[4gou]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='4gou' size='340' side='right'caption='[[4gou]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4gou]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GOU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4gou]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GOU FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fid|4fid]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EHI_010670, EhRGS-RhoGEF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 Entamoeba histolytica])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gou OCA], [https://pdbe.org/4gou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gou RCSB], [https://www.ebi.ac.uk/pdbsum/4gou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gou ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gou OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gou RCSB], [http://www.ebi.ac.uk/pdbsum/4gou PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/C4LYV4_ENTH1 C4LYV4_ENTH1] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 4gou" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Entamoeba histolytica]] | | [[Category: Entamoeba histolytica]] |
| - | [[Category: Bosch, D E]] | + | [[Category: Large Structures]] |
| - | [[Category: Kimple, A J]] | + | [[Category: Bosch DE]] |
| - | [[Category: Machius, M]] | + | [[Category: Kimple AJ]] |
| - | [[Category: Muller, R E]] | + | [[Category: Machius M]] |
| - | [[Category: Siderovski, D P]] | + | [[Category: Muller RE]] |
| - | [[Category: Willard, F S]] | + | [[Category: Siderovski DP]] |
| - | [[Category: Dh domain]]
| + | [[Category: Willard FS]] |
| - | [[Category: Ehgalpha1]]
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| - | [[Category: Gtpase accelerating protein]]
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| - | [[Category: Heterotrimeric g protein effector]]
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| - | [[Category: Ph domain]]
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| - | [[Category: Phospholipid binding]]
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| - | [[Category: Rgs domain]]
| + | |
| - | [[Category: Rho guanine nucleotide exchange factor]]
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| - | [[Category: Signaling protein]]
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| Structural highlights
Function
C4LYV4_ENTH1
Publication Abstract from PubMed
G protein signaling pathways, as key components of physiologic responsiveness and timing, are frequent targets for pharmacologic intervention. Here, we identify an effector for heterotrimeric G protein alpha subunit (EhGalpha1) signaling from Entamoeba histolytica, the causative agent of amoebic colitis. EhGalpha1 interacts with this effector and guanosine triphosphatase-accelerating protein, EhRGS-RhoGEF, in a nucleotide state-selective fashion. Coexpression of EhRGS-RhoGEF with constitutively active EhGalpha1 and EhRacC leads to Rac-dependent spreading in Drosophila S2 cells. EhRGS-RhoGEF overexpression in E. histolytica trophozoites leads to reduced migration toward serum and lower cysteine protease activity, as well as reduced attachment to, and killing of, host cells. A 2.3 A crystal structure of the full-length EhRGS-RhoGEF reveals a putative inhibitory helix engaging the Dbl homology domain Rho-binding surface and the pleckstrin homology domain. Mutational analysis of the EhGalpha1/EhRGS-RhoGEF interface confirms a canonical "regulator of G protein signaling" domain rather than a RhoGEF-RGS ("rgRGS") domain, suggesting a convergent evolution toward heterotrimeric and small G protein cross-talk.
Structural Determinants of RGS-RhoGEF Signaling Critical to Entamoeba histolytica Pathogenesis.,Bosch DE, Kimple AJ, Manning AJ, Muller RE, Willard FS, Machius M, Rogers SL, Siderovski DP Structure. 2012 Dec 19. pii: S0969-2126(12)00427-3. doi:, 10.1016/j.str.2012.11.012. PMID:23260656[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bosch DE, Kimple AJ, Manning AJ, Muller RE, Willard FS, Machius M, Rogers SL, Siderovski DP. Structural Determinants of RGS-RhoGEF Signaling Critical to Entamoeba histolytica Pathogenesis. Structure. 2012 Dec 19. pii: S0969-2126(12)00427-3. doi:, 10.1016/j.str.2012.11.012. PMID:23260656 doi:http://dx.doi.org/10.1016/j.str.2012.11.012
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