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| | ==THREE-DIMENSIONAL STRUCTURE OF BOMBYXIN-II, AN INSULIN-RELATED BRAIN-SECRETORY PEPTIDE OF THE SILKMOTH BOMBYX MORI: COMPARISON WITH INSULIN AND RELAXIN== | | ==THREE-DIMENSIONAL STRUCTURE OF BOMBYXIN-II, AN INSULIN-RELATED BRAIN-SECRETORY PEPTIDE OF THE SILKMOTH BOMBYX MORI: COMPARISON WITH INSULIN AND RELAXIN== |
| - | <StructureSection load='1bom' size='340' side='right' caption='[[1bom]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | + | <StructureSection load='1bom' size='340' side='right'caption='[[1bom]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1bom]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bommo Bommo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BOM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1bom]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BOM FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 1 model</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bon|1bon]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bom OCA], [http://pdbe.org/1bom PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bom RCSB], [http://www.ebi.ac.uk/pdbsum/1bom PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bom OCA], [https://pdbe.org/1bom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bom RCSB], [https://www.ebi.ac.uk/pdbsum/1bom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bom ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BXA2_BOMMO BXA2_BOMMO]] Brain peptide responsible for activation of prothoracic glands to produce ecdysone in insects. [[http://www.uniprot.org/uniprot/BXA6_BOMMO BXA6_BOMMO]] Brain peptide responsible for activation of prothoracic glands to produce ecdysone in insects. | + | [https://www.uniprot.org/uniprot/BXA2_BOMMO BXA2_BOMMO] Brain peptide responsible for activation of prothoracic glands to produce ecdysone in insects. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bommo]] | + | [[Category: Bombyx mori]] |
| - | [[Category: Hatanaka, H]] | + | [[Category: Large Structures]] |
| - | [[Category: Inagaki, F]] | + | [[Category: Hatanaka H]] |
| - | [[Category: Kohda, D]] | + | [[Category: Inagaki F]] |
| - | [[Category: Nagata, K]] | + | [[Category: Kohda D]] |
| - | [[Category: Insulin-like brain-secretory peptide]]
| + | [[Category: Nagata K]] |
| Structural highlights
Function
BXA2_BOMMO Brain peptide responsible for activation of prothoracic glands to produce ecdysone in insects.
Publication Abstract from PubMed
The three-dimensional solution structure of bombyxin-II, an insulin-like two-chain peptide produced by the brain of the silkworm Bombyx mori, has been determined by simulated annealing calculations based on 535 distance constraints and 24 torsion-angle constraints derived from NMR data and three distance constraints of the disulfide bonds. To our knowledge, this is the first three-dimensional structure determined for an invertebrate insulin-related peptide. The root-mean-square deviations between the best 10 structures and the mean structure are 0.58(+/- 0.15) A for the backbone heavy atoms (N, C alpha, C) and 1.03(+/- 0.18) A for all non-hydrogen atom if less well-defined N and C termini (A1, A20, B(-2) to B4 and B23 to B25) are excluded. The overall main-chain structure of bombyxin-II is similar to that of insulin. However, there are significant conformational and functional differences in their B-chain C-terminal parts. The B-chain C-terminal part of bombyxin-II adopts an extension of the B-chain central helix like that of relaxin and is not required for bombyxin activity, while the corresponding part of insulin adopts a sharp turn and a beta-strand and is essential for insulin activity. This structure demonstrates that bombyxin-II is more closely related to relaxin than to insulin, and suggests that insulin might have evolved the additional receptor-recognition site in the B-chain C-terminal beta-strand to distinguish itself from bombyxin and relaxin. The structure of bombyxin-II thus provides novel insights into the receptor recognition and divergent molecular evolution of insulin-superfamily peptides.
Three-dimensional solution structure of bombyxin-II an insulin-like peptide of the silkmoth Bombyx mori: structural comparison with insulin and relaxin.,Nagata K, Hatanaka H, Kohda D, Kataoka H, Nagasawa H, Isogai A, Ishizaki H, Suzuki A, Inagaki F J Mol Biol. 1995 Nov 10;253(5):749-58. PMID:7473749[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nagata K, Hatanaka H, Kohda D, Kataoka H, Nagasawa H, Isogai A, Ishizaki H, Suzuki A, Inagaki F. Three-dimensional solution structure of bombyxin-II an insulin-like peptide of the silkmoth Bombyx mori: structural comparison with insulin and relaxin. J Mol Biol. 1995 Nov 10;253(5):749-58. PMID:7473749 doi:http://dx.doi.org/S0022-2836(85)70588-8
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