1npe

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(New page: 200px<br /><applet load="1npe" size="450" color="white" frame="true" align="right" spinBox="true" caption="1npe, resolution 2.3&Aring;" /> '''Crystal structure of ...)
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[[Image:1npe.jpg|left|200px]]<br /><applet load="1npe" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1npe, resolution 2.3&Aring;" />
 
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'''Crystal structure of Nidogen/Laminin Complex'''<br />
 
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==Overview==
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==Crystal structure of Nidogen/Laminin Complex==
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Basement membranes are fundamental to tissue organization and physiology, in all metazoans. The interaction between laminin and nidogen is crucial, to the assembly of basement membranes. The structure of the interacting, domains reveals a six-bladed Tyr-Trp-Thr-Asp (YWTD) beta-propeller domain, in nidogen bound to laminin epidermal-growth-factor-like (LE) modules, III3-5 in laminin (LE3-5). Laminin LE module 4 binds to an, amphitheatre-shaped surface on the pseudo-6-fold axis of the, beta-propeller, and LE module 3 binds over its rim. A Phe residue that, shutters the water-filled central aperture of the beta-propeller, the, rigidity of the amphitheatre, and high shape complementarity enable the, construction of an evolutionarily conserved binding surface for LE4 of, unprecedentedly high affinity for its small size. Hypermorphic mutations, in the Wnt co-receptor LRP5 (refs 6-9) suggest that a similar YWTD, beta-propeller interface is used to bind ligands that function in, developmental pathways. A related interface, but shifted off-centre from, the pseudo-6-fold axis and lacking the shutter over the central aperture, is used in the low-density lipoprotein receptor for an intramolecular, interaction that is regulated by pH in receptor recycling.
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<StructureSection load='1npe' size='340' side='right'caption='[[1npe]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1npe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NPE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NPE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1npe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1npe OCA], [https://pdbe.org/1npe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1npe RCSB], [https://www.ebi.ac.uk/pdbsum/1npe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1npe ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NID1_MOUSE NID1_MOUSE] Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/1npe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1npe ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Basement membranes are fundamental to tissue organization and physiology in all metazoans. The interaction between laminin and nidogen is crucial to the assembly of basement membranes. The structure of the interacting domains reveals a six-bladed Tyr-Trp-Thr-Asp (YWTD) beta-propeller domain in nidogen bound to laminin epidermal-growth-factor-like (LE) modules III3-5 in laminin (LE3-5). Laminin LE module 4 binds to an amphitheatre-shaped surface on the pseudo-6-fold axis of the beta-propeller, and LE module 3 binds over its rim. A Phe residue that shutters the water-filled central aperture of the beta-propeller, the rigidity of the amphitheatre, and high shape complementarity enable the construction of an evolutionarily conserved binding surface for LE4 of unprecedentedly high affinity for its small size. Hypermorphic mutations in the Wnt co-receptor LRP5 (refs 6-9) suggest that a similar YWTD beta-propeller interface is used to bind ligands that function in developmental pathways. A related interface, but shifted off-centre from the pseudo-6-fold axis and lacking the shutter over the central aperture, is used in the low-density lipoprotein receptor for an intramolecular interaction that is regulated by pH in receptor recycling.
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==About this Structure==
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Complex between nidogen and laminin fragments reveals a paradigmatic beta-propeller interface.,Takagi J, Yang Y, Liu JH, Wang JH, Springer TA Nature. 2003 Aug 21;424(6951):969-74. PMID:12931195<ref>PMID:12931195</ref>
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1NPE is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NPE OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Complex between nidogen and laminin fragments reveals a paradigmatic beta-propeller interface., Takagi J, Yang Y, Liu JH, Wang JH, Springer TA, Nature. 2003 Aug 21;424(6951):969-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12931195 12931195]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 1npe" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Liu, J.H.]]
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[[Category: Springer, T.A.]]
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[[Category: Takagi, J.]]
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[[Category: Wang, J.H.]]
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[[Category: Yang, Y.T.]]
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[[Category: CD]]
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[[Category: basement membrane]]
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[[Category: beta-propeller]]
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[[Category: egf-like]]
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[[Category: glycoprotein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:27:27 2007''
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==See Also==
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*[[Laminin|Laminin]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Liu J-H]]
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[[Category: Springer TA]]
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[[Category: Takagi J]]
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[[Category: Wang J-H]]
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[[Category: Yang YT]]

Current revision

Crystal structure of Nidogen/Laminin Complex

PDB ID 1npe

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