1ukk

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(New page: 200px<br /><applet load="1ukk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ukk, resolution 1.60&Aring;" /> '''Structure of Osmotic...)
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[[Image:1ukk.jpg|left|200px]]<br /><applet load="1ukk" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ukk, resolution 1.60&Aring;" />
 
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'''Structure of Osmotically Inducible Protein C from Thermus thermophilus'''<br />
 
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==Overview==
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==Structure of Osmotically Inducible Protein C from Thermus thermophilus==
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The X-ray crystallographic structure of osmotically inducible Protein C, from the thermophilic bacterium, Thermus thermophilus HB8, was solved to, 1.6A using the multiple wavelength anomalous dispersion method and a, selenomethionine incorporated protein (Se-MAD). The crystal space group, was P1 with cell dimensions of a=37.58 A, b=40.95 A, c=48.14 A, alpha=76.9, degrees, beta=74.0 degrees and gamma=64.1 degrees. The two tightly, interacting monomers in the asymmetric unit are related by a, non-crystallographic 2-fold. The dimer structure is defined primarily by, two very long anti-parallel, over-lapping alpha-helices at the core, with, a further six-stranded anti-parallel beta-sheet on the outside of the, structure. With respect to the beta-sheets, both A and B monomers, contribute three strands each resulting in an intertwining of the, structure. The active site consists of two cysteine residues from one, monomer and an arginine and glutamic acid from the other. Enzymatic assays, have revealed that T.thermophilus OsmC has a hydroperoxide peroxidase, activity.
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<StructureSection load='1ukk' size='340' side='right'caption='[[1ukk]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ukk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UKK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UKK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ukk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ukk OCA], [https://pdbe.org/1ukk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ukk RCSB], [https://www.ebi.ac.uk/pdbsum/1ukk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ukk ProSAT], [https://www.topsan.org/Proteins/RSGI/1ukk TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/P84124_THETH P84124_THETH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uk/1ukk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ukk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The X-ray crystallographic structure of osmotically inducible Protein C from the thermophilic bacterium, Thermus thermophilus HB8, was solved to 1.6A using the multiple wavelength anomalous dispersion method and a selenomethionine incorporated protein (Se-MAD). The crystal space group was P1 with cell dimensions of a=37.58 A, b=40.95 A, c=48.14 A, alpha=76.9 degrees, beta=74.0 degrees and gamma=64.1 degrees. The two tightly interacting monomers in the asymmetric unit are related by a non-crystallographic 2-fold. The dimer structure is defined primarily by two very long anti-parallel, over-lapping alpha-helices at the core, with a further six-stranded anti-parallel beta-sheet on the outside of the structure. With respect to the beta-sheets, both A and B monomers contribute three strands each resulting in an intertwining of the structure. The active site consists of two cysteine residues from one monomer and an arginine and glutamic acid from the other. Enzymatic assays have revealed that T.thermophilus OsmC has a hydroperoxide peroxidase activity.
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==About this Structure==
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Crystallographic structure and biochemical analysis of the Thermus thermophilus osmotically inducible protein C.,Rehse PH, Ohshima N, Nodake Y, Tahirov TH J Mol Biol. 2004 May 14;338(5):959-68. PMID:15111059<ref>PMID:15111059</ref>
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1UKK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with TRS as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UKK OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystallographic structure and biochemical analysis of the Thermus thermophilus osmotically inducible protein C., Rehse PH, Ohshima N, Nodake Y, Tahirov TH, J Mol Biol. 2004 May 14;338(5):959-68. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15111059 15111059]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1ukk" style="background-color:#fffaf0;"></div>
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[[Category: Thermus thermophilus]]
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== References ==
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[[Category: Kuramitsu, S.]]
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<references/>
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[[Category: Miyano, M.]]
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__TOC__
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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</StructureSection>
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[[Category: Rehse, P.H.]]
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[[Category: Large Structures]]
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[[Category: Tahirov, T.H.]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Yokoyama, S.]]
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[[Category: Kuramitsu S]]
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[[Category: TRS]]
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[[Category: Miyano M]]
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[[Category: cysteinesulfinic acid]]
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[[Category: Rehse PH]]
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[[Category: inducible]]
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[[Category: Tahirov TH]]
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[[Category: osmotic]]
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[[Category: Yokoyama S]]
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[[Category: peroxidase]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:08:24 2007''
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Current revision

Structure of Osmotically Inducible Protein C from Thermus thermophilus

PDB ID 1ukk

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