1w9m
From Proteopedia
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| - | [[Image:1w9m.gif|left|200px]] | ||
| - | + | ==AS-isolated hybrid cluster protein from Desulfovibrio vulgaris X-ray structure at 1.35A resolution using iron anomalous signal== | |
| - | + | <StructureSection load='1w9m' size='340' side='right'caption='[[1w9m]], [[Resolution|resolution]] 1.35Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[1w9m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._Hildenborough Desulfovibrio vulgaris str. Hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W9M FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35Å</td></tr> | |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene></td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w9m OCA], [https://pdbe.org/1w9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w9m RCSB], [https://www.ebi.ac.uk/pdbsum/1w9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w9m ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | + | == Function == | |
| - | + | [https://www.uniprot.org/uniprot/HCP_NITV2 HCP_NITV2] Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O.[HAMAP-Rule:MF_00069] | |
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w9/1w9m_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w9m ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The hybrid cluster protein (HCP) from the sulfate-reducing bacterium Desulfovibrio vulgaris strain Hildenborough has been isolated and crystallized anaerobically. The phase problem was solved for a P2(1)2(1)2(1) crystal form using multiple-wavelength anomalous diffraction data collected in the vicinity of the Fe K absorption edge. Although the overall protein structure is essentially the same as that previously obtained, it shows that the nature of the hybrid cluster has particular differences when isolated and crystallized in the absence of oxygen and this provides insight into the structural features associated with changes in the oxidation state. A comparison between HCPs and carbon monoxide dehydrogenases (CoDs) shows that they possess a similar fold and that the dehydrogenases have a related cluster at the equivalent HCP hybrid cluster position. This helps to understand the nature of the hybrid cluster and to predict a dimeric structure for class 3 HCPs, which lack the N-terminal region. | ||
| - | + | Structural and functional relationships in the hybrid cluster protein family: structure of the anaerobically purified hybrid cluster protein from Desulfovibrio vulgaris at 1.35 A resolution.,Aragao D, Mitchell EP, Frazao CF, Carrondo MA, Lindley PF Acta Crystallogr D Biol Crystallogr. 2008 Jun;64(Pt 6):665-74. Epub 2008, May 14. PMID:18560155<ref>PMID:18560155</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1w9m" style="background-color:#fffaf0;"></div> | |
| - | [[Category: Desulfovibrio vulgaris]] | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Aragao | + | __TOC__ |
| - | [[Category: Coelho | + | </StructureSection> |
| - | [[Category: Lindley | + | [[Category: Desulfovibrio vulgaris str. Hildenborough]] |
| - | [[Category: Macedo | + | [[Category: Large Structures]] |
| - | [[Category: Mitchell | + | [[Category: Aragao D]] |
| - | [[Category: Romao | + | [[Category: Coelho D]] |
| - | [[Category: Teixeira | + | [[Category: Lindley PF]] |
| - | + | [[Category: Macedo S]] | |
| - | + | [[Category: Mitchell EP]] | |
| - | + | [[Category: Romao CV]] | |
| - | + | [[Category: Teixeira M]] | |
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Current revision
AS-isolated hybrid cluster protein from Desulfovibrio vulgaris X-ray structure at 1.35A resolution using iron anomalous signal
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