1w9m

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[[Image:1w9m.gif|left|200px]]
 
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==AS-isolated hybrid cluster protein from Desulfovibrio vulgaris X-ray structure at 1.35A resolution using iron anomalous signal==
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The line below this paragraph, containing "STRUCTURE_1w9m", creates the "Structure Box" on the page.
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<StructureSection load='1w9m' size='340' side='right'caption='[[1w9m]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1w9m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._Hildenborough Desulfovibrio vulgaris str. Hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W9M FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene></td></tr>
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{{STRUCTURE_1w9m| PDB=1w9m | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w9m OCA], [https://pdbe.org/1w9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w9m RCSB], [https://www.ebi.ac.uk/pdbsum/1w9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w9m ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HCP_NITV2 HCP_NITV2] Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O.[HAMAP-Rule:MF_00069]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w9/1w9m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w9m ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The hybrid cluster protein (HCP) from the sulfate-reducing bacterium Desulfovibrio vulgaris strain Hildenborough has been isolated and crystallized anaerobically. The phase problem was solved for a P2(1)2(1)2(1) crystal form using multiple-wavelength anomalous diffraction data collected in the vicinity of the Fe K absorption edge. Although the overall protein structure is essentially the same as that previously obtained, it shows that the nature of the hybrid cluster has particular differences when isolated and crystallized in the absence of oxygen and this provides insight into the structural features associated with changes in the oxidation state. A comparison between HCPs and carbon monoxide dehydrogenases (CoDs) shows that they possess a similar fold and that the dehydrogenases have a related cluster at the equivalent HCP hybrid cluster position. This helps to understand the nature of the hybrid cluster and to predict a dimeric structure for class 3 HCPs, which lack the N-terminal region.
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'''AS-ISOLATED HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO VULGARIS X-RAY STRUCTURE AT 1.35A RESOLUTION USING IRON ANOMALOUS SIGNAL'''
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Structural and functional relationships in the hybrid cluster protein family: structure of the anaerobically purified hybrid cluster protein from Desulfovibrio vulgaris at 1.35 A resolution.,Aragao D, Mitchell EP, Frazao CF, Carrondo MA, Lindley PF Acta Crystallogr D Biol Crystallogr. 2008 Jun;64(Pt 6):665-74. Epub 2008, May 14. PMID:18560155<ref>PMID:18560155</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==Overview==
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</div>
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The hybrid cluster proteins from the sulfate reducing bacteria Desulfovibrio desulfuricans ATCC 27774 ( Dd) and Desulfovibrio vulgaris strain Hildenborough ( Dv) have been isolated and crystallized anaerobically. In each case, the protein has been reduced with dithionite and the crystal structure of the reduced form elucidated using X-ray synchrotron radiation techniques at 1.25 A and 1.55 A resolution for Dd and Dv, respectively. Although the overall structures of the proteins are unchanged upon reduction, there are significant changes at the hybrid cluster centres. These include significant movements in the position of the iron atom linked to the persulfide moiety in the oxidized as-isolated proteins and the sulfur atom of the persulfide itself. The nature of these changes is described and the implications with respect to the function of hybrid cluster proteins are discussed.
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<div class="pdbe-citations 1w9m" style="background-color:#fffaf0;"></div>
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== References ==
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==About this Structure==
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<references/>
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1W9M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W9M OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Desulfovibrio vulgaris str. Hildenborough]]
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Reduced hybrid cluster proteins (HCP) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at high resolution using synchrotron radiation., Aragao D, Macedo S, Mitchell EP, Romao CV, Liu MY, Frazao C, Saraiva LM, Xavier AV, LeGall J, van Dongen WM, Hagen WR, Teixeira M, Carrondo MA, Lindley P, J Biol Inorg Chem. 2003 May;8(5):540-8. Epub 2003 Feb 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12764602 12764602]
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[[Category: Large Structures]]
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[[Category: Desulfovibrio vulgaris]]
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[[Category: Aragao D]]
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[[Category: Single protein]]
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[[Category: Coelho D]]
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[[Category: Aragao, D.]]
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[[Category: Lindley PF]]
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[[Category: Coelho, D.]]
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[[Category: Macedo S]]
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[[Category: Lindley, P F.]]
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[[Category: Mitchell EP]]
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[[Category: Macedo, S.]]
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[[Category: Romao CV]]
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[[Category: Mitchell, E P.]]
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[[Category: Teixeira M]]
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[[Category: Romao, C V.]]
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[[Category: Teixeira, M.]]
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[[Category: Anaerobic]]
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[[Category: As-isolated]]
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[[Category: Desulfovibrio vulgari]]
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[[Category: Hybrid cluster protein]]
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[[Category: Oxidised]]
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[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 22:17:01 2008''
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Current revision

AS-isolated hybrid cluster protein from Desulfovibrio vulgaris X-ray structure at 1.35A resolution using iron anomalous signal

PDB ID 1w9m

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