2j5h

From Proteopedia

(Difference between revisions)

Current revision

NMR analysis of mouse CRIPTO CFC domain

Structural highlights

2j5h is a 1 chain structure with sequence from Mus musculus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 10 models
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TDGF1_MOUSE GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by Tmem8a modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells. Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.[UniProtKB:P13385]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We report here for the first time the solution structures at pH 3 and pH 6 of the synthetic CFC domain of mouse Cripto and of the point mutated variant W107A that is unable to bind to the Alk4 Cripto receptor. NMR data confirm that the CFC domain has a C1-C4, C2-C6, C3-C5 disulfide pattern and show that structures are rather flexible and globally extended, with three noncanonical antiparallel strands. His104 and Trp107 side chains protrude from a protein edge and are strongly exposed to solvent, supporting previous evidence of direct involvement in receptor binding. On the opposite molecule side, several nonpolar residues are gathered, forming a large hydrophobic patch that supposedly acts as interface with the cell membrane or the adjacent EGF-like domain. A second hydrophilic patch surrounding His104 and Trp107 is present only in the wild type variant, suggesting a possible involvement in modulating Alk4 recognition.

Solution structure of mouse Cripto CFC domain and its inactive variant Trp107Ala.,Calvanese L, Saporito A, Marasco D, D'Auria G, Minchiotti G, Pedone C, Paolillo L, Falcigno L, Ruvo M J Med Chem. 2006 Nov 30;49(24):7054-62. PMID:17125258^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Calvanese L, Saporito A, Marasco D, D'Auria G, Minchiotti G, Pedone C, Paolillo L, Falcigno L, Ruvo M. Solution structure of mouse Cripto CFC domain and its inactive variant Trp107Ala. J Med Chem. 2006 Nov 30;49(24):7054-62. PMID:17125258 doi:10.1021/jm060772r

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2j5h"

@@ Line 1: / Line 1: @@
 ==NMR analysis of mouse CRIPTO CFC domain==
-<StructureSection load='2j5h' size='340' side='right'caption='[[2j5h]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+<StructureSection load='2j5h' size='340' side='right'caption='[[2j5h]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2j5h]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J5H FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2j5h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J5H FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j5h OCA], [https://pdbe.org/2j5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j5h RCSB], [https://www.ebi.ac.uk/pdbsum/2j5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j5h ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/TDGF1_MOUSE TDGF1_MOUSE] GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by Tmem8a modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells. Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.[UniProtKB:P13385]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 12: / Line 15: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j5/2j5h_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 34: / Line 37: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Auria, G D]]
+[[Category: Mus musculus]]
-[[Category: Calvanese, L]]
+[[Category: Calvanese L]]
-[[Category: Falcigno, L]]
+[[Category: D'Auria G]]
-[[Category: Marasco, D]]
+[[Category: Falcigno L]]
-[[Category: Minchiotti, G]]
+[[Category: Marasco D]]
-[[Category: Paolillo, L]]
+[[Category: Minchiotti G]]
-[[Category: Pedone, C]]
+[[Category: Paolillo L]]
-[[Category: Ruvo, M]]
+[[Category: Pedone C]]
-[[Category: Saporito, A]]
+[[Category: Ruvo M]]
-[[Category: Cripto]]
+[[Category: Saporito A]]
-[[Category: Cysteine-rich domain]]
-[[Category: Egf-cfc family]]
-[[Category: Growth factor]]
-[[Category: Hormone-growth factor complex]]
-[[Category: Hormone/growth factor]]
-[[Category: Tumour progression]]

2j5h

From Proteopedia

Current revision

NMR analysis of mouse CRIPTO CFC domain

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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