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| | ==Solution NMR Structure of xenopus Fn14== | | ==Solution NMR Structure of xenopus Fn14== |
| - | <StructureSection load='2kn0' size='340' side='right' caption='[[2kn0]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2kn0' size='340' side='right'caption='[[2kn0]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2kn0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KN0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KN0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2kn0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KN0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KN0 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2kmz|2kmz]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Fn14, tnfrsf12a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kn0 OCA], [https://pdbe.org/2kn0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kn0 RCSB], [https://www.ebi.ac.uk/pdbsum/2kn0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kn0 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kn0 OCA], [http://pdbe.org/2kn0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kn0 RCSB], [http://www.ebi.ac.uk/pdbsum/2kn0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kn0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q6SIX7_XENLA Q6SIX7_XENLA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: African clawed frog]] | + | [[Category: Large Structures]] |
| - | [[Category: Cuervo, H]] | + | [[Category: Xenopus laevis]] |
| - | [[Category: Day, E S]] | + | [[Category: Cuervo H]] |
| - | [[Category: Krushinskie, D]] | + | [[Category: Day ES]] |
| - | [[Category: Pellegrini, M]] | + | [[Category: Krushinskie D]] |
| - | [[Category: Perroud, M]] | + | [[Category: Pellegrini M]] |
| - | [[Category: Schneider, P]] | + | [[Category: Perroud M]] |
| - | [[Category: Strauch, K]] | + | [[Category: Schneider P]] |
| - | [[Category: Sun, Y]] | + | [[Category: Strauch K]] |
| - | [[Category: Willen, L]] | + | [[Category: Sun Y]] |
| - | [[Category: Zheng, T S]] | + | [[Category: Willen L]] |
| - | [[Category: Apoptosis]]
| + | [[Category: Zheng TS]] |
| - | [[Category: Crd]]
| + | |
| - | [[Category: Fn14]]
| + | |
| - | [[Category: Mutagenesis]]
| + | |
| - | [[Category: Tnf receptor]]
| + | |
| - | [[Category: Tweak]]
| + | |
| Structural highlights
Function
Q6SIX7_XENLA
Publication Abstract from PubMed
TWEAK (TNF homologue with weak apoptosis-inducing activity) and Fn14 (fibroblast growth factor-inducible protein 14) are members of the tumor necrosis factor (TNF) ligand and receptor super-families. Having observed that Xenopus Fn14 cross-reacts with human TWEAK, despite its relatively low sequence homology to human Fn14, we examined the conservation in tertiary fold and binding interfaces between the two species. Our results, combining NMR solution structure determination, binding assays, extensive site-directed mutagenesis and molecular modeling, reveal that, in addition to the known and previously characterized beta-hairpin motif, the helix-loop-helix motif makes an essential contribution to the receptor/ligand binding interface. We further discuss the insight provided by the structural analyses regarding how the cysteine-rich domains of the TNF receptor super-family may have evolved over time. DATABASE: Structural data are available in the Protein Data Bank/BioMagResBank databases under the accession codes 2KMZ, 2KN0 and 2KN1 and 17237, 17247 and 17252. STRUCTURED DIGITAL ABSTRACT: TWEAK binds to hFn14 by surface plasmon resonance (View interaction) xeFn14 binds to TWEAK by enzyme linked immunosorbent assay (View interaction) TWEAK binds to xeFn14 by surface plasmon resonance (View interaction) hFn14 binds to TWEAK by enzyme linked immunosorbent assay (View interaction).
Structure of the extracellular domains of human and Xenopus Fn14: implications in the evolution of TWEAK and Fn14 interactions.,Pellegrini M, Willen L, Perroud M, Krushinskie D, Strauch K, Cuervo H, Day ES, Schneider P, Zheng TS FEBS J. 2013 Feb 25. doi: 10.1111/febs.12206. PMID:23438059[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pellegrini M, Willen L, Perroud M, Krushinskie D, Strauch K, Cuervo H, Day ES, Schneider P, Zheng TS. Structure of the extracellular domains of human and Xenopus Fn14: implications in the evolution of TWEAK and Fn14 interactions. FEBS J. 2013 Feb 25. doi: 10.1111/febs.12206. PMID:23438059 doi:http://dx.doi.org/10.1111/febs.12206
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