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| | ==Crystal structure of addiction module from Mycobacterial species== | | ==Crystal structure of addiction module from Mycobacterial species== |
| - | <StructureSection load='4xgr' size='340' side='right' caption='[[4xgr]], [[Resolution|resolution]] 2.70Å' scene=''> | + | <StructureSection load='4xgr' size='340' side='right'caption='[[4xgr]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xgr]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XGR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XGR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xgr]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XGR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XGR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xgq|4xgq]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xgr OCA], [https://pdbe.org/4xgr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xgr RCSB], [https://www.ebi.ac.uk/pdbsum/4xgr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xgr ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xgr OCA], [http://pdbe.org/4xgr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xgr RCSB], [http://www.ebi.ac.uk/pdbsum/4xgr PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/VPC30_MYCTU VPC30_MYCTU]] Toxic component of a type II toxin-antitoxin (TA) module. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB30.[HAMAP-Rule:MF_00265]<ref>PMID:20011113</ref> [[http://www.uniprot.org/uniprot/VPB30_MYCTU VPB30_MYCTU]] Antitoxin component of a type II toxin-antitoxin (TA) module. Upon expression in M.smegmatis neutralizes the effect of cognate toxin VapC30. | + | [https://www.uniprot.org/uniprot/VPC30_MYCTU VPC30_MYCTU] Toxic component of a type II toxin-antitoxin (TA) module. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB30.[HAMAP-Rule:MF_00265]<ref>PMID:20011113</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lee, B J]] | + | [[Category: Large Structures]] |
| - | [[Category: Lee, I G]] | + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| - | [[Category: Lee, S J]] | + | [[Category: Lee B-J]] |
| - | [[Category: Addiction module]] | + | [[Category: Lee I-G]] |
| - | [[Category: Mycobacteria]] | + | [[Category: Lee SJ]] |
| - | [[Category: Toxin-antitoxin complex]]
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| Structural highlights
Function
VPC30_MYCTU Toxic component of a type II toxin-antitoxin (TA) module. An RNase (By similarity). Upon expression in M.smegmatis inhibits colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin VapB30.[HAMAP-Rule:MF_00265][1]
Publication Abstract from PubMed
Toxin-antitoxin (TA) systems play important roles in bacterial physiology, such as multidrug tolerance, biofilm formation, and arrest of cellular growth under stress conditions. To develop novel antimicrobial agents against tuberculosis, we focused on VapBC systems, which encompass more than half of TA systems in Mycobacterium tuberculosis. Here, we report that theMycobacterium tuberculosis VapC30 toxin regulates cellular growth through both magnesium and manganese ion-dependent ribonuclease activity and is inhibited by the cognate VapB30 antitoxin. We also determined the 2.7-A resolution crystal structure of the M. tuberculosis VapBC30 complex, which revealed a novel process of inactivation of the VapC30 toxin via swapped blocking by the VapB30 antitoxin. Our study on M. tuberculosis VapBC30 leads us to design two kinds of VapB30 and VapC30-based novel peptides which successfully disrupt the toxin-antitoxin complex and thus activate the ribonuclease activity of the VapC30 toxin. Our discovery herein possibly paves the way to treat tuberculosis for next generation.
Structural and functional studies of the Mycobacterium tuberculosis VapBC30 toxin-antitoxin system: implications for the design of novel antimicrobial peptides.,Lee IG, Lee SJ, Chae S, Lee KY, Kim JH, Lee BJ Nucleic Acids Res. 2015 Jul 6. pii: gkv689. PMID:26150422[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ramage HR, Connolly LE, Cox JS. Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009 Dec;5(12):e1000767. doi: 10.1371/journal.pgen.1000767. Epub 2009, Dec 11. PMID:20011113 doi:http://dx.doi.org/10.1371/journal.pgen.1000767
- ↑ Lee IG, Lee SJ, Chae S, Lee KY, Kim JH, Lee BJ. Structural and functional studies of the Mycobacterium tuberculosis VapBC30 toxin-antitoxin system: implications for the design of novel antimicrobial peptides. Nucleic Acids Res. 2015 Jul 6. pii: gkv689. PMID:26150422 doi:http://dx.doi.org/10.1093/nar/gkv689
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