5x6a

Structural highlights

Function

LP9B_ASPFU Lytic polysaccharide monooxygenase (LPMO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 and C4 oxidation products (PubMed:28750348, PubMed:31237199, PubMed:33541573). Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate (PubMed:31237199). Shows activity on phosphoric acid swollen cellulose, on NaOH pretreated soy spent flakes as well as on crystalline cellulose (Avicel) (PubMed:28750348). Does not have a positive effect on cel6A activity, but acts synergistically with endoglucanase egl7 (PubMed:33383972).^{[1] [2] [3] [4]}

References

1. ↑ Pierce BC, Agger JW, Zhang Z, Wichmann J, Meyer AS. A comparative study on the activity of fungal lytic polysaccharide monooxygenases for the depolymerization of cellulose in soybean spent flakes. Carbohydr Res. 2017 Sep 8;449:85-94. PMID:28750348 doi:10.1016/j.carres.2017.07.004
2. ↑ de Gouvêa PF, Gerolamo LE, Bernardi AV, Pereira LMS, Uyemura SA, Dinamarco TM. Lytic Polysaccharide Monooxygenase from Aspergillus fumigatus can Improve Enzymatic Cocktail Activity During Sugarcane Bagasse Hydrolysis. Protein Pept Lett. 2019;26(5):377-385. PMID:31237199 doi:10.2174/0929866526666190228163629
3. ↑ Bernardi AV, Gerolamo LE, de Gouvêa PF, Yonamine DK, Pereira LMS, de Oliveira AHC, Uyemura SA, Dinamarco TM. LPMO AfAA9_B and Cellobiohydrolase AfCel6A from A. fumigatus Boost Enzymatic Saccharification Activity of Cellulase Cocktail. Int J Mol Sci. 2020 Dec 29;22(1):276. PMID:33383972 doi:10.3390/ijms22010276
4. ↑ Velasco J, de Oliveira Arnoldi Pellegrini V, Sepulchro AGV, Kadowaki MAS, Santo MCE, Polikarpov I, Segato F. Comparative analysis of two recombinant LPMOs from Aspergillus fumigatus and their effects on sugarcane bagasse saccharification. Enzyme Microb Technol. 2021 Mar;144:109746. PMID:33541573 doi:10.1016/j.enzmictec.2021.109746