7kgc
From Proteopedia
(Difference between revisions)
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<StructureSection load='7kgc' size='340' side='right'caption='[[7kgc]], [[Resolution|resolution]] 1.95Å' scene=''> | <StructureSection load='7kgc' size='340' side='right'caption='[[7kgc]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'> | + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KGC FirstGlance]. <br> |
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kgc OCA], [https://pdbe.org/7kgc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kgc RCSB], [https://www.ebi.ac.uk/pdbsum/7kgc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kgc ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kgc OCA], [https://pdbe.org/7kgc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kgc RCSB], [https://www.ebi.ac.uk/pdbsum/7kgc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kgc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | == Function == | ||
| - | [https://www.uniprot.org/uniprot/A2DAL5_TRIV3 A2DAL5_TRIV3] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | To understand whether protein Tv-PSP1 from Trichomonas vaginalis recognizes mRNA parasite stem-loop structures, we conducted REMSA and intrinsic fluorescence assays. We found the recombinant Tv-PSP1 structure, determined with X-ray crystallography, showed unusual thermal stability of the quaternary structure, associated with a disulfide bridge CYS76-CYS104. To gain deeper insight into the Tv-PSP1 interaction with mRNA stem-loops (mRNAsl) and its relationship with thermal stability, we also used an integrated computational protocol that combined molecular dynamics simulations, docking assays, and binding energy calculations. Docking models allowed us to determine a putative contact surface interaction region between Tv-PSP1 and mRNAsl. We determined the contributions of these complexes to the binding free energy (DeltaG(b)) in the electrostatic (DeltaG(elec)) and nonelectrostatic (DeltaG(non-elec)) components using the Adaptive Poisson-Boltzmann Solver (APBS) program. We are the first, to the best of our knowledge, to show the interaction between Tv-PSP1 and the stem-loop structures of mRNA. | ||
| - | |||
| - | A Putative New Role of Tv-PSP1 Recognizes IRE and ERE Hairpin Structures from Trichomonas vaginalis.,Millan-Pacheco C, Arreola R, Villalobos-Osnaya A, Garza-Ramos G, Serratos IN, Diaz-Vilchis A, Rudino-Pinera E, Alvarez-Sanchez ME Pathogens. 2023 Jan 3;12(1):79. doi: 10.3390/pathogens12010079. PMID:36678426<ref>PMID:36678426</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 7kgc" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Trichomonas vaginalis]] | ||
[[Category: Alvarez-Sanchez ME]] | [[Category: Alvarez-Sanchez ME]] | ||
[[Category: Arreola R]] | [[Category: Arreola R]] | ||
[[Category: Diaz-Vilchis A]] | [[Category: Diaz-Vilchis A]] | ||
[[Category: Rudino-Pinera E]] | [[Category: Rudino-Pinera E]] | ||
Current revision
Crystal structure of a perchloric acid-soluble protein (PSP) from Trichomonas vaginalis at 1.95 A
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