1e3m

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{{Seed}}
 
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[[Image:1e3m.png|left|200px]]
 
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==The crystal structure of E. coli MutS binding to DNA with a G:T mismatch==
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The line below this paragraph, containing "STRUCTURE_1e3m", creates the "Structure Box" on the page.
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<StructureSection load='1e3m' size='340' side='right'caption='[[1e3m]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1e3m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E3M FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_1e3m| PDB=1e3m | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3m OCA], [https://pdbe.org/1e3m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e3m RCSB], [https://www.ebi.ac.uk/pdbsum/1e3m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e3m ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MUTS_ECOLI MUTS_ECOLI] This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e3/1e3m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e3m ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DNA mismatch repair ensures genomic integrity on DNA replication. Recognition of a DNA mismatch by a dimeric MutS protein initiates a cascade of reactions and results in repair of the newly synthesized strand; however, details of the molecular mechanism remain controversial. Here we present the crystal structure at 2.2 A of MutS from Escherichia coli bound to a G x T mismatch. The two MutS monomers have different conformations and form a heterodimer at the structural level. Only one monomer recognizes the mismatch specifically and has ADP bound. Mismatch recognition occurs by extensive minor groove interactions causing unusual base pairing and kinking of the DNA. Nonspecific major groove DNA-binding domains from both monomers embrace the DNA in a clamp-like structure. The interleaved nucleotide-binding sites are located far from the DNA. Mutations in human MutS alpha (MSH2/MSH6) that lead to hereditary predisposition for cancer, such as hereditary non-polyposis colorectal cancer, can be mapped to this crystal structure.
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===THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A G:T MISMATCH===
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The crystal structure of DNA mismatch repair protein MutS binding to a G x T mismatch.,Lamers MH, Perrakis A, Enzlin JH, Winterwerp HH, de Wind N, Sixma TK Nature. 2000 Oct 12;407(6805):711-7. PMID:11048711<ref>PMID:11048711</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1e3m" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_11048711}}, adds the Publication Abstract to the page
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*[[DNA mismatch repair protein 3D structures|DNA mismatch repair protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 11048711 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11048711}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1E3M is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3M OCA].
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==Reference==
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<ref group="xtra">PMID:11048711</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Enzlin, J.]]
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[[Category: Large Structures]]
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[[Category: Lamers, M H.]]
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[[Category: De Wind N]]
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[[Category: Perrakis, A.]]
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[[Category: Enzlin JH]]
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[[Category: Sixma, T K.]]
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[[Category: Lamers MH]]
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[[Category: Wind, N De.]]
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[[Category: Perrakis A]]
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[[Category: Winterwerp, H H.K.]]
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[[Category: Sixma TK]]
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[[Category: Dna binding]]
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[[Category: Winterwerp HHK]]
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[[Category: Mismatch recognition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:07:29 2009''
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Current revision

The crystal structure of E. coli MutS binding to DNA with a G:T mismatch

PDB ID 1e3m

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