1gni

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[[Image:1gni.gif|left|200px]]<br />
 
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<applet load="1gni" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gni, resolution 2.40&Aring;" />
 
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'''HUMAN SERUM ALBUMIN COMPLEXED WITH CIS-9-OCTADECENOIC ACID (OLEIC ACID)'''<br />
 
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==Overview==
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==HUMAN SERUM ALBUMIN COMPLEXED WITH cis-9-OCTADECENOIC ACID (OLEIC ACID)==
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The primary ligands of human serum albumin (HSA), an abundant plasma, protein, are non-esterified fatty acids. In vivo, the majority of fatty, acids associated with the protein are unsaturated. We present here the, first high-resolution crystal structures of HSA complexed with two, important unsaturated fatty acids, the monounsaturated oleic acid (C18:1), and the polyunsaturated arachidonic acid (C20:4). Both compounds are, observed to occupy the seven binding sites distributed across the protein, that are also bound by medium and long-chain saturated fatty acids., Although C18:1 fatty acid binds each site on HSA in a conformation almost, identical with that of the corresponding saturated compound (C18:0), the, presence of multiple cis double bonds in C20:4 induces distinct binding, configurations at some sites. The observed restriction on binding, configurations plausibly accounts for differences in the pattern of, binding affinities for the primary sites between polyunsaturated fatty, acids and their saturated or monounsaturated counterparts.
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<StructureSection load='1gni' size='340' side='right'caption='[[1gni]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gni]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GNI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gni OCA], [https://pdbe.org/1gni PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gni RCSB], [https://www.ebi.ac.uk/pdbsum/1gni PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gni ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN] Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:[https://omim.org/entry/103600 103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.<ref>PMID:8048949</ref> <ref>PMID:7852505</ref> <ref>PMID:9329347</ref> <ref>PMID:9589637</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN] Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.<ref>PMID:19021548</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gn/1gni_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gni ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The primary ligands of human serum albumin (HSA), an abundant plasma protein, are non-esterified fatty acids. In vivo, the majority of fatty acids associated with the protein are unsaturated. We present here the first high-resolution crystal structures of HSA complexed with two important unsaturated fatty acids, the monounsaturated oleic acid (C18:1) and the polyunsaturated arachidonic acid (C20:4). Both compounds are observed to occupy the seven binding sites distributed across the protein that are also bound by medium and long-chain saturated fatty acids. Although C18:1 fatty acid binds each site on HSA in a conformation almost identical with that of the corresponding saturated compound (C18:0), the presence of multiple cis double bonds in C20:4 induces distinct binding configurations at some sites. The observed restriction on binding configurations plausibly accounts for differences in the pattern of binding affinities for the primary sites between polyunsaturated fatty acids and their saturated or monounsaturated counterparts.
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==About this Structure==
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Crystal structures of human serum albumin complexed with monounsaturated and polyunsaturated fatty acids.,Petitpas I, Grune T, Bhattacharya AA, Curry S J Mol Biol. 2001 Dec 14;314(5):955-60. PMID:11743713<ref>PMID:11743713</ref>
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1GNI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with OLA as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: OL1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GNI OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structures of human serum albumin complexed with monounsaturated and polyunsaturated fatty acids., Petitpas I, Grune T, Bhattacharya AA, Curry S, J Mol Biol. 2001 Dec 14;314(5):955-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11743713 11743713]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 1gni" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Bhattacharya, A.A.]]
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[[Category: Curry, S.]]
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[[Category: Gruene, T.]]
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[[Category: Petitpas, I.]]
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[[Category: OLA]]
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[[Category: glycoprotein]]
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[[Category: lipid-binding]]
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[[Category: metal-binding]]
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[[Category: plasma protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 16:15:42 2007''
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==See Also==
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*[[Albumin 3D structures|Albumin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Bhattacharya AA]]
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[[Category: Curry S]]
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[[Category: Gruene T]]
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[[Category: Petitpas I]]

Current revision

HUMAN SERUM ALBUMIN COMPLEXED WITH cis-9-OCTADECENOIC ACID (OLEIC ACID)

PDB ID 1gni

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