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| | ==PROTEINASE INHIBITOR PMP-C (NMR, 36 STRUCTURES)== | | ==PROTEINASE INHIBITOR PMP-C (NMR, 36 STRUCTURES)== |
| - | <StructureSection load='1pmc' size='340' side='right' caption='[[1pmc]], [[NMR_Ensembles_of_Models | 36 NMR models]]' scene=''> | + | <StructureSection load='1pmc' size='340' side='right'caption='[[1pmc]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1pmc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Locusta_migratoria Locusta migratoria]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PMC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PMC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1pmc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Locusta_migratoria Locusta migratoria]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PMC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PMC FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pmc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pmc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1pmc RCSB], [http://www.ebi.ac.uk/pdbsum/1pmc PDBsum]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 36 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pmc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pmc OCA], [https://pdbe.org/1pmc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pmc RCSB], [https://www.ebi.ac.uk/pdbsum/1pmc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pmc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/LCM_LOCMI LCM_LOCMI] Both LCMI I and II are inhibitors of chymotrypsin and elastase (in vitro). They both inhibit the prophenol oxidase activation cascade. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 1pmc" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Locusta migratoria]] | | [[Category: Locusta migratoria]] |
| - | [[Category: Hietter, H]] | + | [[Category: Hietter H]] |
| - | [[Category: Lefevre, J F]] | + | [[Category: Lefevre J-F]] |
| - | [[Category: Mer, G]] | + | [[Category: Mer G]] |
| - | [[Category: Calcium channel blocker]]
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| - | [[Category: Proteinase inhibitor]]
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| Structural highlights
Function
LCM_LOCMI Both LCMI I and II are inhibitors of chymotrypsin and elastase (in vitro). They both inhibit the prophenol oxidase activation cascade.
Publication Abstract from PubMed
The solution structure and the disulfide pairings of a 36-residue proteinase inhibitor isolated from the insect Locusta migratoria have been determined using NMR spectroscopy and simulated annealing calculations. The peptide, termed PMP-C, was previously shown to inhibit bovine alpha-chymotrypsin as well as human leukocyte elastase, and was also found to block high-voltage-activated Ca2+ currents in rat sensory neurones. PMP-C has a prolate ellipsoid shape and adopts a tertiary fold hitherto unobserved in the large group of small "canonical" proteinase inhibitors. The over-all fold consists mainly of three strands arranged in a right-handed twisted, antiparallel, beta-sheet that demarcates a cavity, together with a linear amino-terminal segment oriented almost perpendicular to the three strands of the beta-sheet. Inside the cavity a phenyl ring constitutes the centre of a hydrophobic core. The proteinase binding loop is located in the carboxy-terminal part of the molecule, between two cysteine residues involved in disulfide bridges. Its conformation resembles that found in other small canonical proteinase inhibitors. A comparison of PMP-C structure with the recently published solution structure of the related peptide PMP-D2 shows that the most significant differences are complementary changes involved in the stabilization of similar folds. This comparison led us to review the structure of PMP-D2 and to identify two salt bridges in PMP-D2.
Solution structure of PMP-C: a new fold in the group of small serine proteinase inhibitors.,Mer G, Hietter H, Kellenberger C, Renatus M, Luu B, Lefevre JF J Mol Biol. 1996 Apr 26;258(1):158-71. PMID:8613985[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Mer G, Hietter H, Kellenberger C, Renatus M, Luu B, Lefevre JF. Solution structure of PMP-C: a new fold in the group of small serine proteinase inhibitors. J Mol Biol. 1996 Apr 26;258(1):158-71. PMID:8613985 doi:http://dx.doi.org/S0022-2836(96)90240-5
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