1psk

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(New page: 200px<br /> <applet load="1psk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1psk, resolution 2.8&Aring;" /> '''THE CRYSTAL STRUCTUR...)
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[[Image:1psk.gif|left|200px]]<br />
 
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<applet load="1psk" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1psk, resolution 2.8&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF AN FAB FRAGMENT THAT BINDS TO THE MELANOMA-ASSOCIATED GD2 GANGLIOSIDE'''<br />
 
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==Overview==
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==THE CRYSTAL STRUCTURE OF AN FAB FRAGMENT THAT BINDS TO THE MELANOMA-ASSOCIATED GD2 GANGLIOSIDE==
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The GD2 ganglioside is a cell-surface component that appears on the, surface of metastatic melanoma cells and is a marker for the progression, of the disease. The ME36.1 monoclonal antibody binds to the GD2, ganglioside and has shown potential as a therapeutic antibody. ME36.1 is a, possible alternative therapy to radiation, which is often ineffective in, late-stage melanoma. The crystal structure of the Fab fragment of ME36.1, has been determined using molecular replacement and refined to an R factor, of 20.4% at 2.8 A resolution. The model has good geometry with, root-mean-square deviations of 0.008 A from ideal bond lengths and 1.7, degrees from ideal bond angles. The crystal structure of the ME36.1 Fab, shows that its complementarity determining region forms a groove-shaped, binding site rather than the pocket-type observed in other sugar binding, Fabs. Molecular modeling has placed a four-residue sugar, representative, of GD2, in the antigen binding site. The GD2 sugar moiety is stabilized by, a network of hydrogen bonds that define the specificity of ME36.1 toward, its antigen.
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<StructureSection load='1psk' size='340' side='right'caption='[[1psk]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1psk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PSK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1psk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1psk OCA], [https://pdbe.org/1psk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1psk RCSB], [https://www.ebi.ac.uk/pdbsum/1psk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1psk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IGKC_MOUSE IGKC_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/1psk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1psk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The GD2 ganglioside is a cell-surface component that appears on the surface of metastatic melanoma cells and is a marker for the progression of the disease. The ME36.1 monoclonal antibody binds to the GD2 ganglioside and has shown potential as a therapeutic antibody. ME36.1 is a possible alternative therapy to radiation, which is often ineffective in late-stage melanoma. The crystal structure of the Fab fragment of ME36.1 has been determined using molecular replacement and refined to an R factor of 20.4% at 2.8 A resolution. The model has good geometry with root-mean-square deviations of 0.008 A from ideal bond lengths and 1.7 degrees from ideal bond angles. The crystal structure of the ME36.1 Fab shows that its complementarity determining region forms a groove-shaped binding site rather than the pocket-type observed in other sugar binding Fabs. Molecular modeling has placed a four-residue sugar, representative of GD2, in the antigen binding site. The GD2 sugar moiety is stabilized by a network of hydrogen bonds that define the specificity of ME36.1 toward its antigen.
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==About this Structure==
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The crystal structure of a Fab fragment to the melanoma-associated GD2 ganglioside.,Pichla SL, Murali R, Burnett RM J Struct Biol. 1997 Jun;119(1):6-16. PMID:9216084<ref>PMID:9216084</ref>
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1PSK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PSK OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The crystal structure of a Fab fragment to the melanoma-associated GD2 ganglioside., Pichla SL, Murali R, Burnett RM, J Struct Biol. 1997 Jun;119(1):6-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9216084 9216084]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 1psk" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Burnett, R.M.]]
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[[Category: Murali, R.]]
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[[Category: Pichla, S.L.]]
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[[Category: carbohydrate]]
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[[Category: fab]]
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[[Category: gd2-ganglioside]]
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[[Category: immunoglobulin]]
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[[Category: melanoma]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:39:47 2007''
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==See Also==
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Burnett RM]]
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[[Category: Murali R]]
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[[Category: Pichla SL]]

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THE CRYSTAL STRUCTURE OF AN FAB FRAGMENT THAT BINDS TO THE MELANOMA-ASSOCIATED GD2 GANGLIOSIDE

PDB ID 1psk

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