1uoy

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(New page: 200px<br /><applet load="1uoy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uoy, resolution 1.50&Aring;" /> '''THE BUBBLE PROTEIN F...)
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[[Image:1uoy.jpg|left|200px]]<br /><applet load="1uoy" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1uoy, resolution 1.50&Aring;" />
 
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'''THE BUBBLE PROTEIN FROM PENICILLIUM BREVICOMPACTUM DIERCKX EXUDATE.'''<br />
 
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==Overview==
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==The bubble protein from Penicillium brevicompactum Dierckx exudate.==
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A small cysteine-rich protein, the function of which remains elusive, was, discovered in the exudate of a Penicillium species. Crystal diffraction, experiments conducted using in-house Cu Kalpha radiation and an R-AXIS, IV++ imaging-plate detector yielded high-quality data to 1.4 A, with a, distinguishable anomalous signal from sulfur (DeltaF/F = 0.031). This was, used to phase the data and solve the structure using a single data set;, the 64-residue amino-acid sequence was unambiguously determined from the, electron density. It revealed a globular all-beta protein with a hitherto, unknown fold, having a surface electrostatic charge distribution that is, similar to that of another small secreted fungal protein, the Williopsis, mrakii killer toxin. Aligning the charge distribution superimposed the, potential recognition sites of the two proteins, suggesting a similar, negatively charged target.
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<StructureSection load='1uoy' size='340' side='right'caption='[[1uoy]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1uoy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Penicillium_brevicompactum Penicillium brevicompactum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UOY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uoy OCA], [https://pdbe.org/1uoy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uoy RCSB], [https://www.ebi.ac.uk/pdbsum/1uoy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uoy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BUBL_PENBR BUBL_PENBR] May act as a toxin. May recognize a molecule or part of a molecule with a negatively charged surface potential.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A small cysteine-rich protein, the function of which remains elusive, was discovered in the exudate of a Penicillium species. Crystal diffraction experiments conducted using in-house Cu Kalpha radiation and an R-AXIS IV++ imaging-plate detector yielded high-quality data to 1.4 A, with a distinguishable anomalous signal from sulfur (DeltaF/F = 0.031). This was used to phase the data and solve the structure using a single data set; the 64-residue amino-acid sequence was unambiguously determined from the electron density. It revealed a globular all-beta protein with a hitherto unknown fold, having a surface electrostatic charge distribution that is similar to that of another small secreted fungal protein, the Williopsis mrakii killer toxin. Aligning the charge distribution superimposed the potential recognition sites of the two proteins, suggesting a similar negatively charged target.
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==About this Structure==
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Solving the structure of the bubble protein using the anomalous sulfur signal from single-crystal in-house Cu Kalpha diffraction data only.,Olsen JG, Flensburg C, Olsen O, Bricogne G, Henriksen A Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):250-5. Epub 2004, Jan 23. PMID:14747700<ref>PMID:14747700</ref>
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1UOY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Penicillium_brevicompactum Penicillium brevicompactum]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UOY OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solving the structure of the bubble protein using the anomalous sulfur signal from single-crystal in-house Cu Kalpha diffraction data only., Olsen JG, Flensburg C, Olsen O, Bricogne G, Henriksen A, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):250-5. Epub 2004, Jan 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14747700 14747700]
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</div>
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<div class="pdbe-citations 1uoy" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Penicillium brevicompactum]]
[[Category: Penicillium brevicompactum]]
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[[Category: Single protein]]
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[[Category: Bricogne G]]
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[[Category: Bricogne, G.]]
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[[Category: Flensburg C]]
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[[Category: Flensburg, C.]]
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[[Category: Henriksen A]]
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[[Category: Henriksen, A.]]
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[[Category: Olsen JG]]
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[[Category: Olsen, J.G.]]
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[[Category: Olsen O]]
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[[Category: Olsen, O.]]
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[[Category: Seibold M]]
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[[Category: Seibold, M.]]
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[[Category: exudate protein]]
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[[Category: potential killer toxin]]
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[[Category: sulfur phasing]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:16:57 2007''
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Current revision

The bubble protein from Penicillium brevicompactum Dierckx exudate.

PDB ID 1uoy

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