1v7p

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{{Seed}}
 
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[[Image:1v7p.png|left|200px]]
 
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==Structure of EMS16-alpha2-I domain complex==
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The line below this paragraph, containing "STRUCTURE_1v7p", creates the "Structure Box" on the page.
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<StructureSection load='1v7p' size='340' side='right'caption='[[1v7p]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1v7p]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Echis_multisquamatus Echis multisquamatus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The February 2011 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Integrin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2011_2 10.2210/rcsb_pdb/mom_2011_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V7P FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_1v7p| PDB=1v7p | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v7p OCA], [https://pdbe.org/1v7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v7p RCSB], [https://www.ebi.ac.uk/pdbsum/1v7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v7p ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SLA_ECHML SLA_ECHML] EMS16 is a potent and selective inhibitor of alpha-2/beta-1 (ITGA2/ITGB1) integrin and acts as a potent antagonist of platelet aggregation and cell migration. Binds specifically to the I domain of the alpha-2 subunit, in a metal ion-independent fashion.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v7/1v7p_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1v7p ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Snake venoms contain a number of heterodimeric C-type lectin-like proteins (CLPs) that interact specifically with components of the haemostatic system. EMS16 from the venom of Echis multisquamatus binds to the collagen receptor, integrin alpha2beta1, also known as glycoprotein (GP) Ia/IIa, and specifically inhibits collagen binding. Here we report the crystal structure of EMS16 in complex with recombinant integrin alpha2-I domain that plays a central role in collagen binding. The structure of the complex at 1.9 Angstrom resolution reveals that the collagen-binding site of the alpha2-I domain is covered completely by the bound EMS16. This blockage by EMS16 appears to spatially inhibit collagen binding to the alpha2-I domain. The bound alpha2-I domain adopts a closed conformation, which is seen in the absence of ligand, suggesting that EMS16 stabilizes a closed conformation corresponding to the less active structure of the alpha2-I domain. EMS16 does not directly bind to the manganese ion and residues of the metal ion-dependent adhesion site (MIDAS) of the alpha2-I domain, suggesting that EMS16 may have the potential to bind specifically to the alpha2-I domain in a metal ion-independent fashion.
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===Structure of EMS16-alpha2-I domain complex===
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Crystal structure of EMS16 in complex with the integrin alpha2-I domain.,Horii K, Okuda D, Morita T, Mizuno H J Mol Biol. 2004 Aug 6;341(2):519-27. PMID:15276841<ref>PMID:15276841</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1v7p" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15276841}}, adds the Publication Abstract to the page
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*[[Integrin 3D structures|Integrin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15276841 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15276841}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1V7P is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Echis_multisquamatus Echis multisquamatus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V7P OCA].
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==Reference==
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Crystal structure of EMS16 in complex with the integrin alpha2-I domain., Horii K, Okuda D, Morita T, Mizuno H, J Mol Biol. 2004 Aug 6;341(2):519-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15276841 15276841]
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Structural characterization of EMS16, an antagonist of collagen receptor (GPIa/IIa) from the venom of Echis multisquamatus., Horii K, Okuda D, Morita T, Mizuno H, Biochemistry. 2003 Nov 4;42(43):12497-502. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14580195 14580195]
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Crystal structure of the I domain from integrin alpha2beta1., Emsley J, King SL, Bergelson JM, Liddington RC, J Biol Chem. 1997 Nov 7;272(45):28512-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9353312 9353312]
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Structural basis of collagen recognition by integrin alpha2beta1., Emsley J, Knight CG, Farndale RW, Barnes MJ, Liddington RC, Cell. 2000 Mar 31;101(1):47-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10778855 10778855]
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[[Category: Echis multisquamatus]]
[[Category: Echis multisquamatus]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
 
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[[Category: Horii, K.]]
 
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[[Category: Mizuno, H.]]
 
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[[Category: Morita, T.]]
 
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[[Category: Okuda, D.]]
 
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[[Category: Antagonist]]
 
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[[Category: C-type lectin]]
 
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[[Category: Cell adhesion]]
 
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[[Category: Glycoprotein]]
 
[[Category: Integrin]]
[[Category: Integrin]]
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[[Category: Snake venom]]
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:49:34 2008''
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[[Category: Horii K]]
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[[Category: Mizuno H]]
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[[Category: Morita T]]
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[[Category: Okuda D]]

Current revision

Structure of EMS16-alpha2-I domain complex

PDB ID 1v7p

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