1w9a

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[[Image:1w9a.png|left|200px]]
 
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==Crystal structure of Rv1155 from Mycobacterium tuberculosis==
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The line below this paragraph, containing "STRUCTURE_1w9a", creates the "Structure Box" on the page.
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<StructureSection load='1w9a' size='340' side='right'caption='[[1w9a]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1w9a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W9A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W9A FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_1w9a| PDB=1w9a | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w9a OCA], [https://pdbe.org/1w9a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w9a RCSB], [https://www.ebi.ac.uk/pdbsum/1w9a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w9a ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/F420R_MYCTU F420R_MYCTU] F420H(2)-dependent reductase able to catalyze the reduction of biliverdin-IXalpha to bilirubin-IXalpha in vitro. However, kinetic parameters show that it is less efficient than the biliverdin reductase Rv2074 and suggest biliverdin-IXalpha is unlikely to be the native substrate of Rv1155, which probably catalyzes the reduction of an alternative molecule in vivo (PubMed:27364382). Binds coenzyme F420, but does not bind FMN or other flavins (PubMed:25644473). Cannot use pyridoxine 5'-phosphate, pyridoxamine 5'-phosphate, pyridoxal 5'-phosphate (PLP), the anti-tuberculosis drug PA-824 or aflatoxin analogs as substrates (PubMed:25644473).<ref>PMID:25644473</ref> <ref>PMID:27364382</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w9/1w9a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w9a ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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With the aim of elucidating the biological function of hypothetical proteins unique amongst the Actynomyces sub-group of bacteria, we have solved the crystal structure of the conserved hypothetical protein Rv1155 from Mycobacterium tuberculosis at 1.8 A resolution. Rv1155 is a homodimer both in the crystal structure and in solution and folds into two separate domains consisting of a six-stranded anti-parallel beta-barrel fold flanked by two alpha-helices and a helix-turn-helix domain. Both domains contribute to the formation of two deep clefts at the dimer interface. The overall fold of Rv1155 strikingly resembles that of flavin mononucleotide-binding protein and pyridoxamine 5'-phosphate oxydase, but the architecture of the putative binding pocket is markedly different, consistent with the lack of color of Rv1155 and its inability to bind FMN. Rv1155 thus appears to belong to a group of proteins with stringent conservation of the binding cleft, having evolved towards a new binding function.
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===CRYSTAL STRUCTURE OF RV1155 FROM MYCOBACTERIUM TUBERCULOSIS===
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Crystal structure of the conserved hypothetical protein Rv1155 from Mycobacterium tuberculosis.,Canaan S, Sulzenbacher G, Roig-Zamboni V, Scappuccini-Calvo L, Frassinetti F, Maurin D, Cambillau C, Bourne Y FEBS Lett. 2005 Jan 3;579(1):215-21. PMID:15620716<ref>PMID:15620716</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15620716}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1w9a" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15620716 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15620716}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[1w9a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W9A OCA].
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Bourne Y]]
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==Reference==
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[[Category: Cambillau C]]
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<ref group="xtra">PMID:015620716</ref><references group="xtra"/>
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[[Category: Cannan S]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Frassinetti F]]
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[[Category: Bourne, Y.]]
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[[Category: Maurien D]]
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[[Category: Cambillau, C.]]
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[[Category: Roig-Zamboni V]]
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[[Category: Canaan, S.]]
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[[Category: Scappuccini L]]
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[[Category: Frassinetti-Calvo, F.]]
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[[Category: Sulzenbacher G]]
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[[Category: Maurin, D.]]
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[[Category: Roig-Zamboni, V.]]
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[[Category: Scappuccini, L.]]
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[[Category: Sulzenbacher, G.]]
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[[Category: Hypothetical protein]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Related to fmn-binding protein]]
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[[Category: Structural genomic]]
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[[Category: Unknown function]]
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Current revision

Crystal structure of Rv1155 from Mycobacterium tuberculosis

PDB ID 1w9a

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