2e4z

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2e4z" size="450" color="white" frame="true" align="right" spinBox="true" caption="2e4z, resolution 3.30&Aring;" /> '''Crystal structure of...)
Current revision (07:52, 23 October 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2e4z.jpg|left|200px]]<br /><applet load="2e4z" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2e4z, resolution 3.30&Aring;" />
 
-
'''Crystal structure of the ligand-binding region of the group III metabotropic glutamate receptor'''<br />
 
-
==Overview==
+
==Crystal structure of the ligand-binding region of the group III metabotropic glutamate receptor==
-
Metabotropic glutamate receptors play major roles in the activation of, excitatory synapses in the central nerve system. We determined the crystal, structure of the entire extracellular region of the group II receptor and, that of the ligand-binding region of the group III receptor. A comparison, among groups I, II, and III provides the structural basis that could, account for the discrimination of group-specific agonists. Furthermore, the structure of group II includes the cysteine-rich domain, which is, tightly linked to the ligand-binding domain by a disulfide bridge, suggesting a potential role in transmitting a ligand-induced, conformational change into the downstream transmembrane region. The, structure also reveals the lateral interaction between the two, cysteine-rich domains, which could stimulate clustering of the dimeric, receptors on the cell surface. We propose a general activation mechanism, of the dimeric receptor coupled with both ligand-binding and interprotomer, rearrangements.
+
<StructureSection load='2e4z' size='340' side='right'caption='[[2e4z]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2e4z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E4Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E4Z FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e4z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e4z OCA], [https://pdbe.org/2e4z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e4z RCSB], [https://www.ebi.ac.uk/pdbsum/2e4z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e4z ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/GRM7_RAT GRM7_RAT] G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.<ref>PMID:8145723</ref> <ref>PMID:8288585</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e4/2e4z_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e4z ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Metabotropic glutamate receptors play major roles in the activation of excitatory synapses in the central nerve system. We determined the crystal structure of the entire extracellular region of the group II receptor and that of the ligand-binding region of the group III receptor. A comparison among groups I, II, and III provides the structural basis that could account for the discrimination of group-specific agonists. Furthermore, the structure of group II includes the cysteine-rich domain, which is tightly linked to the ligand-binding domain by a disulfide bridge, suggesting a potential role in transmitting a ligand-induced conformational change into the downstream transmembrane region. The structure also reveals the lateral interaction between the two cysteine-rich domains, which could stimulate clustering of the dimeric receptors on the cell surface. We propose a general activation mechanism of the dimeric receptor coupled with both ligand-binding and interprotomer rearrangements.
-
==About this Structure==
+
Structures of the extracellular regions of the group II/III metabotropic glutamate receptors.,Muto T, Tsuchiya D, Morikawa K, Jingami H Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):3759-64. Epub 2007 Feb 26. PMID:17360426<ref>PMID:17360426</ref>
-
2E4Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with MES as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2E4Z OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Structures of the extracellular regions of the group II/III metabotropic glutamate receptors., Muto T, Tsuchiya D, Morikawa K, Jingami H, Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):3759-64. Epub 2007 Feb 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17360426 17360426]
+
</div>
-
[[Category: Rattus norvegicus]]
+
<div class="pdbe-citations 2e4z" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
-
[[Category: Jingami, H.]]
+
-
[[Category: Morikawa, K.]]
+
-
[[Category: Muto, T.]]
+
-
[[Category: Tsuchiya, D.]]
+
-
[[Category: MES]]
+
-
[[Category: central nerve system]]
+
-
[[Category: g-protein-coupled receptor]]
+
-
[[Category: neuron]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:57:08 2007''
+
==See Also==
 +
*[[Metabotropic glutamate receptor 3D structures|Metabotropic glutamate receptor 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Rattus norvegicus]]
 +
[[Category: Jingami H]]
 +
[[Category: Morikawa K]]
 +
[[Category: Muto T]]
 +
[[Category: Tsuchiya D]]

Current revision

Crystal structure of the ligand-binding region of the group III metabotropic glutamate receptor

PDB ID 2e4z

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2e4z"
Personal tools