2vjy

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{{Seed}}
 
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[[Image:2vjy.jpg|left|200px]]
 
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==Pyruvate decarboxylase from Kluyveromyces lactis in complex with the substrate analogue methyl acetylphosphonate==
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The line below this paragraph, containing "STRUCTURE_2vjy", creates the "Structure Box" on the page.
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<StructureSection load='2vjy' size='340' side='right'caption='[[2vjy]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2vjy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VJY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALK:(1R)-1-[DIHYDROXY(METHOXY)-LAMBDA^5^-PHOSPHANYL]ETHANOL'>ALK</scene>, <scene name='pdbligand=ALU:METHYL+HYDROGEN+(S)-ACETYLPHOSPHONATE'>ALU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
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{{STRUCTURE_2vjy| PDB=2vjy | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vjy OCA], [https://pdbe.org/2vjy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vjy RCSB], [https://www.ebi.ac.uk/pdbsum/2vjy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vjy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PDC1_KLULA PDC1_KLULA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vj/2vjy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vjy ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The mechanism by which the enzyme pyruvate decarboxylase from two yeast species is activated allosterically has been elucidated. A total of seven three-dimensional structures of the enzyme, of enzyme variants, or of enzyme complexes from two yeast species, three of them reported here for the first time, provide detailed atomic resolution snapshots along the activation coordinate. The prime event is the covalent binding of the substrate pyruvate to the side chain of cysteine 221, thus forming a thiohemiketal. This reaction causes the shift of a neighboring amino acid, which eventually leads to the rigidification of two otherwise flexible loops, one of which provides two histidine residues necessary to complete the enzymatically competent active site architecture. The structural data are complemented and supported by kinetic investigations and binding studies, providing a consistent picture of the structural changes occurring upon enzyme activation.
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===PYRUVATE DECARBOXYLASE FROM KLUYVEROMYCES LACTIS IN COMPLEX WITH THE SUBSTRATE ANALOGUE METHYL ACETYLPHOSPHONATE===
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Covalently bound substrate at the regulatory site of yeast pyruvate decarboxylases triggers allosteric enzyme activation.,Kutter S, Weiss MS, Wille G, Golbik R, Spinka M, Konig S J Biol Chem. 2009 May 1;284(18):12136-44. Epub 2009 Feb 26. PMID:19246454<ref>PMID:19246454</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2vjy" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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2VJY is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VJY OCA].
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*[[Pyruvate decarboxylase|Pyruvate decarboxylase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Kluyveromyces lactis]]
[[Category: Kluyveromyces lactis]]
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[[Category: Pyruvate decarboxylase]]
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[[Category: Large Structures]]
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[[Category: Konig, S.]]
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[[Category: Konig S]]
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[[Category: Kutter, S.]]
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[[Category: Kutter S]]
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[[Category: Weiss, M S.]]
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[[Category: Weiss MS]]
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[[Category: Wille, G.]]
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[[Category: Wille G]]
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[[Category: Asymmetric active site]]
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[[Category: Decarboxylase]]
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[[Category: Dimer of dimer]]
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[[Category: Flavoprotein]]
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[[Category: Lyase]]
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[[Category: Magnesium]]
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[[Category: Map]]
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[[Category: Metal-binding]]
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[[Category: Methyl acetylphosphonate]]
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[[Category: Methylacetylphosphonate]]
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[[Category: Pyruvate]]
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[[Category: Substrate activation]]
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[[Category: Thiamine diphosphate]]
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[[Category: Thiamine pyrophosphate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 28 09:35:33 2009''
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Current revision

Pyruvate decarboxylase from Kluyveromyces lactis in complex with the substrate analogue methyl acetylphosphonate

PDB ID 2vjy

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