2wn9

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{{Seed}}
 
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[[Image:2wn9.jpg|left|200px]]
 
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==Crystal structure of Aplysia ACHBP in complex with 4-0H-DMXBA==
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The line below this paragraph, containing "STRUCTURE_2wn9", creates the "Structure Box" on the page.
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<StructureSection load='2wn9' size='340' side='right'caption='[[2wn9]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wn9]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WN9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WN9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZY5:4-[(E)-5,6-DIHYDRO-2,3-BIPYRIDIN-3(4H)-YLIDENEMETHYL]-3-METHOXYPHENOL'>ZY5</scene></td></tr>
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{{STRUCTURE_2wn9| PDB=2wn9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wn9 OCA], [https://pdbe.org/2wn9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wn9 RCSB], [https://www.ebi.ac.uk/pdbsum/2wn9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wn9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8WSF8_APLCA Q8WSF8_APLCA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wn/2wn9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wn9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The pentameric acetylcholine-binding protein (AChBP) is a soluble surrogate of the ligand binding domain of nicotinic acetylcholine receptors. Agonists bind within a nest of aromatic side chains contributed by loops C and F on opposing faces of each subunit interface. Crystal structures of Aplysia AChBP bound with the agonist anabaseine, two partial agonists selectively activating the alpha7 receptor, 3-(2,4-dimethoxybenzylidene)-anabaseine and its 4-hydroxy metabolite, and an indole-containing partial agonist, tropisetron, were solved at 2.7-1.75 A resolution. All structures identify the Trp 147 carbonyl oxygen as the hydrogen bond acceptor for the agonist-protonated nitrogen. In the partial agonist complexes, the benzylidene and indole substituent positions, dictated by tight interactions with loop F, preclude loop C from adopting the closed conformation seen for full agonists. Fluctuation in loop C position and duality in ligand binding orientations suggest molecular bases for partial agonism at full-length receptors. This study, while pointing to loop F as a major determinant of receptor subtype selectivity, also identifies a new template region for designing alpha7-selective partial agonists to treat cognitive deficits in mental and neurodegenerative disorders.
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===CRYSTAL STRUCTURE OF APLYSIA ACHBP IN COMPLEX WITH 4-0H-DMXBA===
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Structural determinants for interaction of partial agonists with acetylcholine binding protein and neuronal alpha7 nicotinic acetylcholine receptor.,Hibbs RE, Sulzenbacher G, Shi J, Talley TT, Conrod S, Kem WR, Taylor P, Marchot P, Bourne Y EMBO J. 2009 Oct 7;28(19):3040-51. Epub 2009 Aug 20. PMID:19696737<ref>PMID:19696737</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2wn9" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19696737}}, adds the Publication Abstract to the page
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*[[Acetylcholine binding protein 3D structures|Acetylcholine binding protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19696737 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19696737}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2WN9 is a 5 chains structure of sequences from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WN9 OCA].
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==Reference==
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<ref group="xtra">PMID:19696737</ref><references group="xtra"/>
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[[Category: Aplysia californica]]
[[Category: Aplysia californica]]
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[[Category: Bourne, Y.]]
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[[Category: Large Structures]]
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[[Category: Conrod, S.]]
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[[Category: Bourne Y]]
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[[Category: Hibbs, R.]]
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[[Category: Conrod S]]
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[[Category: Kem, W.]]
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[[Category: Hibbs R]]
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[[Category: Marchot, P.]]
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[[Category: Kem W]]
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[[Category: Shi, J.]]
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[[Category: Marchot P]]
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[[Category: Sulzenbacher, G.]]
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[[Category: Shi J]]
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[[Category: Talley, T.]]
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[[Category: Sulzenbacher G]]
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[[Category: Taylor, P.]]
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[[Category: Talley T]]
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[[Category: 4-0h-dmxba]]
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[[Category: Taylor P]]
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[[Category: Acetylcholine binding protein]]
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[[Category: Receptor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 16:00:59 2009''
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Current revision

Crystal structure of Aplysia ACHBP in complex with 4-0H-DMXBA

PDB ID 2wn9

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