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1tq2
From Proteopedia
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[[Image:1tq2.jpg|left|200px]] | [[Image:1tq2.jpg|left|200px]] | ||
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'''Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases''' | '''Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases''' | ||
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[[Category: Uthaiah, R.]] | [[Category: Uthaiah, R.]] | ||
[[Category: Wolf, E.]] | [[Category: Wolf, E.]] | ||
| - | [[Category: | + | [[Category: Crystal structure]] |
| - | [[Category: | + | [[Category: Dimer]] |
| - | [[Category: | + | [[Category: Gtpase]] |
| - | [[Category: | + | [[Category: Immunology]] |
| - | [[Category: | + | [[Category: Interferon gamma]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:14:31 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:14, 3 May 2008
Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases
Overview
Interferon-inducible p47 GTPases are critical mediators of cell-autonomous resistance against several intracellular pathogens. Here we present the first crystal structure of a member of this novel GTPase family, IIGP1, in its nucleotide-free, GDP-, and GppNHp-bound form. The structure shows a Ras-like G domain between an N-terminal three-helix bundle and a complex system of C-terminal helices and loops. Sequence comparison and secondary structure prediction suggest the IIGP1 structure to be a valid model for the p47 GTPase family. The IIGP1 crystals contain a noncrystallographic dimer. We show that the dimer is required for cooperative GTP hydrolysis and GTP-dependent oligomerization of IIGP1. We also present the GDP- and GppNHp-bound monomeric structures of two dimer interface mutants. Our structures direct approaches to the analysis of the catalytic mechanism of IIGP1 and provide a coherent basis for structure-function studies aimed at elucidating the mechanistic basis of pathogen resistance caused by these enigmatic GTPases.
About this Structure
1TQ2 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal structure of IIGP1: a paradigm for interferon-inducible p47 resistance GTPases., Ghosh A, Uthaiah R, Howard J, Herrmann C, Wolf E, Mol Cell. 2004 Sep 10;15(5):727-39. PMID:15350217 Page seeded by OCA on Sat May 3 10:14:31 2008
Categories: Mus musculus | Single protein | Ghosh, A. | Herrmann, C. | Howard, J. | Uthaiah, R. | Wolf, E. | Crystal structure | Dimer | Gtpase | Immunology | Interferon gamma
