8fbe
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of OrfX1 from Clostridium botulinum E1== | |
| + | <StructureSection load='8fbe' size='340' side='right'caption='[[8fbe]], [[Resolution|resolution]] 1.73Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8fbe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum_E1_str._'BoNT_E_Beluga' Clostridium botulinum E1 str. 'BoNT E Beluga']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FBE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FBE FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fbe OCA], [https://pdbe.org/8fbe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fbe RCSB], [https://www.ebi.ac.uk/pdbsum/8fbe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fbe ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/O52975_CLOBO O52975_CLOBO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Botulinum neurotoxins (BoNTs) are among the most lethal toxins known to humans, comprising seven established serotypes termed BoNT/A-G encoded in two types of gene clusters (ha and orfX) in BoNT-producing clostridia. The ha cluster encodes four non-toxic neurotoxin-associated proteins (NAPs) that assemble with BoNTs to protect and enhance their oral toxicity. However, the structure and function of the orfX-type NAPs remain largely unknown. Here, we report the crystal structures for OrfX1, OrfX2, and an OrfX1-OrfX3 complex, which are encoded in the orfX cluster of a BoNT/E1-producing Clostridium botulinum strain associated with human foodborne botulism. These structures lay the foundation for future studies on the potential roles of OrfX proteins in oral intoxication and pathogenesis of BoNTs. | ||
| - | + | Crystal structures of OrfX1, OrfX2 and the OrfX1-OrfX3 complex from the orfX gene cluster of botulinum neurotoxin E1.,Gao L, Lam KH, Liu S, Przykopanski A, Lubke J, Qi R, Kruger M, Nowakowska MB, Selby K, Douillard FP, Dorner MB, Perry K, Lindstrom M, Dorner BG, Rummel A, Jin R FEBS Lett. 2023 Feb;597(4):524-537. doi: 10.1002/1873-3468.14576. Epub 2023 Jan , 27. PMID:36653893<ref>PMID:36653893</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8fbe" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Clostridium botulinum E1 str. 'BoNT E Beluga']] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Gao L]] | ||
| + | [[Category: Liu S]] | ||
Current revision
Crystal structure of OrfX1 from Clostridium botulinum E1
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