1dlf

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(New page: 200px<br /> <applet load="1dlf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dlf, resolution 1.45&Aring;" /> '''HIGH RESOLUTION CRY...)
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[[Image:1dlf.gif|left|200px]]<br />
 
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<applet load="1dlf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dlf, resolution 1.45&Aring;" />
 
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'''HIGH RESOLUTION CRYSTAL STRUCTURE OF THE FV FRAGMENT FROM AN ANTI-DANSYL SWITCH VARIANT ANTIBODY IGG2A(S) CRYSTALLIZED AT PH 5.25'''<br />
 
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==Overview==
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==HIGH RESOLUTION CRYSTAL STRUCTURE OF THE FV FRAGMENT FROM AN ANTI-DANSYL SWITCH VARIANT ANTIBODY IGG2A(S) CRYSTALLIZED AT PH 5.25==
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The Fv fragment from an anti-dansyl antibody was optimally crystallized, into two crystal forms having slightly different lattice dimensions at pH, 5.25 and 6.75. The two crystal structures were determined and refined at, high resolution at 112 K (at 1.45 A for the crystal at pH 5.25 and at 1.55, A for that at pH 6.75). In the two crystal structures, marked differences, were identified in the first half of CDRH3 s having an amino acid sequence, of Ile95H-Tyr96H-Tyr97H-His98H-Tyr99H-Pro1 00H-Trp100aH-Phe100bH-Ala101H-, Tyr102H. NMR pH titration experiments revealed the p Kavalues of four, histidine residues (His27dL, His93L, His55H and His98H) exposed to, solvent. Only His98H (p Ka=6.3) completely changed its protonation state, between the two crystallization conditions. In addition, the environmental, structures including hydration water molecules around the four histidine, residues were carefully compared. While the hydration structures around, His27dL, His93L and His55H were almost invariant between the two crystal, structures, those around His98Hs showed great difference in spite of the, small conformational difference of His98H between the two crystal, structures. These spectroscopic and crystallographic findings suggested, that the change in the protonation state in His98H was responsible for the, structural differences between pH 5.25 and 6.75. In addition, the most, plausible binding site of the dansyl group was mapped into the present, structural models with our previous NMR experimental results. The, complementarity-determining regions H1, H3 and the N-terminal region in, the VH domain formed the site. The side-chain of Tyr96H occupied the site, and interacted with Phe27H of H1, giving a clue for the binding mode of, the dansyl group in the site.
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<StructureSection load='1dlf' size='340' side='right'caption='[[1dlf]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dlf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DLF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DLF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dlf OCA], [https://pdbe.org/1dlf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dlf RCSB], [https://www.ebi.ac.uk/pdbsum/1dlf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dlf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KV2A7_MOUSE KV2A7_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dl/1dlf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dlf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Fv fragment from an anti-dansyl antibody was optimally crystallized into two crystal forms having slightly different lattice dimensions at pH 5.25 and 6.75. The two crystal structures were determined and refined at high resolution at 112 K (at 1.45 A for the crystal at pH 5.25 and at 1.55 A for that at pH 6.75). In the two crystal structures, marked differences were identified in the first half of CDRH3 s having an amino acid sequence of Ile95H-Tyr96H-Tyr97H-His98H-Tyr99H-Pro1 00H-Trp100aH-Phe100bH-Ala101H- Tyr102H. NMR pH titration experiments revealed the p Kavalues of four histidine residues (His27dL, His93L, His55H and His98H) exposed to solvent. Only His98H (p Ka=6.3) completely changed its protonation state between the two crystallization conditions. In addition, the environmental structures including hydration water molecules around the four histidine residues were carefully compared. While the hydration structures around His27dL, His93L and His55H were almost invariant between the two crystal structures, those around His98Hs showed great difference in spite of the small conformational difference of His98H between the two crystal structures. These spectroscopic and crystallographic findings suggested that the change in the protonation state in His98H was responsible for the structural differences between pH 5.25 and 6.75. In addition, the most plausible binding site of the dansyl group was mapped into the present structural models with our previous NMR experimental results. The complementarity-determining regions H1, H3 and the N-terminal region in the VH domain formed the site. The side-chain of Tyr96H occupied the site and interacted with Phe27H of H1, giving a clue for the binding mode of the dansyl group in the site.
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==About this Structure==
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The pH-dependent structural variation of complementarity-determining region H3 in the crystal structures of the Fv fragment from an anti-dansyl monoclonal antibody.,Nakasako M, Takahashi H, Shimba N, Shimada I, Arata Y J Mol Biol. 1999 Aug 6;291(1):117-34. PMID:10438610<ref>PMID:10438610</ref>
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1DLF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DLF OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The pH-dependent structural variation of complementarity-determining region H3 in the crystal structures of the Fv fragment from an anti-dansyl monoclonal antibody., Nakasako M, Takahashi H, Shimba N, Shimada I, Arata Y, J Mol Biol. 1999 Aug 6;291(1):117-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10438610 10438610]
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</div>
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<div class="pdbe-citations 1dlf" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Arata Y]]
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[[Category: Arata, Y.]]
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[[Category: Nakasako M]]
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[[Category: Nakasako, M.]]
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[[Category: Shimada I]]
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[[Category: Shimada, I.]]
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[[Category: Takahashi H]]
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[[Category: Takahashi, H.]]
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[[Category: SO4]]
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[[Category: fv fragment]]
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[[Category: immunoglobulin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:28:32 2007''
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Current revision

HIGH RESOLUTION CRYSTAL STRUCTURE OF THE FV FRAGMENT FROM AN ANTI-DANSYL SWITCH VARIANT ANTIBODY IGG2A(S) CRYSTALLIZED AT PH 5.25

PDB ID 1dlf

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