1dth

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[[Image:1dth.gif|left|200px]]<br />
 
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<applet load="1dth" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dth, resolution 2.0&Aring;" />
 
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'''METALLOPROTEASE'''<br />
 
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==Overview==
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==METALLOPROTEASE==
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Matrix metalloproteinase enzymes have been implicated in degenerative, processes like tumor cell invasion, metastasis, and arthritis. Specific, metalloproteinase inhibitors have been used to block tumor cell, proliferation. We have examined the interaction of batimastat (BB-94) with, a metalloproteinase [atrolysin C (Ht-d), EC 3.4.24.42] active site at, 2.0-angstroms resolution (R = 16.8%). The title structure exhibits an, unexpected binding geometry, with the thiophene ring deeply inserted into, the primary specificity site. This unprecedented binding geometry, dramatizes the significance of the cavernous primary specificity site, pointing the way for the design of a new generation of potential antitumor, drugs.
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<StructureSection load='1dth' size='340' side='right'caption='[[1dth]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dth]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Crotalus_atrox Crotalus atrox]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DTH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DTH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BAT:4-(N-HYDROXYAMINO)-2R-ISOBUTYL-2S-(2-THIENYLTHIOMETHYL)SUCCINYL-L-PHENYLALANINE-N-METHYLAMIDE'>BAT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dth OCA], [https://pdbe.org/1dth PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dth RCSB], [https://www.ebi.ac.uk/pdbsum/1dth PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dth ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VM1AD_CROAT VM1AD_CROAT] Snake venom zinc metalloproteinase that causes hemorrhage by provoking the degradation of the sub-endothelial matrix proteins (fibronectin, laminin, type IV collagen, nidogen, and gelatins).<ref>PMID:2817904</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dt/1dth_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dth ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Matrix metalloproteinase enzymes have been implicated in degenerative processes like tumor cell invasion, metastasis, and arthritis. Specific metalloproteinase inhibitors have been used to block tumor cell proliferation. We have examined the interaction of batimastat (BB-94) with a metalloproteinase [atrolysin C (Ht-d), EC 3.4.24.42] active site at 2.0-angstroms resolution (R = 16.8%). The title structure exhibits an unexpected binding geometry, with the thiophene ring deeply inserted into the primary specificity site. This unprecedented binding geometry dramatizes the significance of the cavernous primary specificity site, pointing the way for the design of a new generation of potential antitumor drugs.
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==About this Structure==
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Batimastat, a potent matrix mealloproteinase inhibitor, exhibits an unexpected mode of binding.,Botos I, Scapozza L, Zhang D, Liotta LA, Meyer EF Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2749-54. PMID:8610113<ref>PMID:8610113</ref>
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1DTH is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Crotalus_atrox Crotalus atrox]] with ZN, CA and BAT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Atrolysin_C Atrolysin C]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.42 3.4.24.42]]. Structure known Active Sites: CAA, CAB, ZNA and ZNB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DTH OCA]].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Batimastat, a potent matrix mealloproteinase inhibitor, exhibits an unexpected mode of binding., Botos I, Scapozza L, Zhang D, Liotta LA, Meyer EF, Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2749-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8610113 8610113]
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</div>
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[[Category: Atrolysin C]]
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<div class="pdbe-citations 1dth" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Crotalus atrox]]
[[Category: Crotalus atrox]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Botos, I.]]
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[[Category: Botos I]]
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[[Category: Liotta, L.A.]]
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[[Category: Liotta LA]]
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[[Category: Meyer, E.F.]]
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[[Category: Meyer EF]]
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[[Category: Scapozza, L.]]
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[[Category: Scapozza L]]
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[[Category: Zhang, D.]]
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[[Category: Zhang D]]
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[[Category: BAT]]
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[[Category: CA]]
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[[Category: ZN]]
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[[Category: hydrolase]]
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[[Category: metalloprotease]]
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[[Category: venom]]
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[[Category: zinc]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:30:23 2007''
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Current revision

METALLOPROTEASE

PDB ID 1dth

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