1f3d

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[[Image:1f3d.jpg|left|200px]]<br /><applet load="1f3d" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1f3d, resolution 1.87&Aring;" />
 
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'''CATALYTIC ANTIBODY 4B2 IN COMPLEX WITH ITS AMIDINIUM HAPTEN.'''<br />
 
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==Overview==
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==CATALYTIC ANTIBODY 4B2 IN COMPLEX WITH ITS AMIDINIUM HAPTEN.==
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The crystal structure of the complex of a catalytic antibody with its, cationic hapten at 1.9-A resolution demonstrates that the hapten amidinium, group is stabilized through an ionic pair interaction with the carboxylate, of a combining-site residue. The location of this carboxylate allows it to, act as a general base in an allylic rearrangement. When compared with, structures of other antibody complexes in which the positive moiety of the, hapten is stabilized mostly by cation-pi interactions, this structure, shows that the amidinium moiety is a useful candidate to elicit a, carboxylate in an antibody combining site at a predetermined location with, respect to the hapten. More generally, this structure highlights the, advantage of a bidentate hapten for the programmed positioning of a, chemically reactive residue in an antibody through charge complementarity, to the hapten.
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<StructureSection load='1f3d' size='340' side='right'caption='[[1f3d]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1f3d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F3D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F3D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TPM:2-(4-AMINOBENZYLAMINO)-3,4,5,6-TETRAHYDROPYRIDINIUM'>TPM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f3d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f3d OCA], [https://pdbe.org/1f3d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f3d RCSB], [https://www.ebi.ac.uk/pdbsum/1f3d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f3d ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A2NHM3_MOUSE A2NHM3_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f3/1f3d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f3d ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the complex of a catalytic antibody with its cationic hapten at 1.9-A resolution demonstrates that the hapten amidinium group is stabilized through an ionic pair interaction with the carboxylate of a combining-site residue. The location of this carboxylate allows it to act as a general base in an allylic rearrangement. When compared with structures of other antibody complexes in which the positive moiety of the hapten is stabilized mostly by cation-pi interactions, this structure shows that the amidinium moiety is a useful candidate to elicit a carboxylate in an antibody combining site at a predetermined location with respect to the hapten. More generally, this structure highlights the advantage of a bidentate hapten for the programmed positioning of a chemically reactive residue in an antibody through charge complementarity to the hapten.
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==About this Structure==
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Structural evidence for a programmed general base in the active site of a catalytic antibody.,Golinelli-Pimpaneau B, Goncalves O, Dintinger T, Blanchard D, Knossow M, Tellier C Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):9892-5. PMID:10963661<ref>PMID:10963661</ref>
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1F3D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=TPM:'>TPM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F3D OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural evidence for a programmed general base in the active site of a catalytic antibody., Golinelli-Pimpaneau B, Goncalves O, Dintinger T, Blanchard D, Knossow M, Tellier C, Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):9892-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10963661 10963661]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 1f3d" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Blanchard, D.]]
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[[Category: Dintinger, T.]]
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[[Category: Golinelli-Pimpaneau, B.]]
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[[Category: Goncalves, O.]]
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[[Category: Knossow, M.]]
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[[Category: Tellier, C.]]
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[[Category: SO4]]
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[[Category: TPM]]
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[[Category: amidinium]]
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[[Category: catalytic antibody]]
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[[Category: haptenic charge]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:45:33 2008''
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==See Also==
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Blanchard D]]
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[[Category: Dintinger T]]
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[[Category: Golinelli-Pimpaneau B]]
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[[Category: Goncalves O]]
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[[Category: Knossow M]]
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[[Category: Tellier C]]

Current revision

CATALYTIC ANTIBODY 4B2 IN COMPLEX WITH ITS AMIDINIUM HAPTEN.

PDB ID 1f3d

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