1f90

From Proteopedia

(Difference between revisions)

Current revision

FAB FRAGMENT OF MONOCLONAL ANTIBODY (LNKB-2) AGAINST HUMAN INTERLEUKIN-2 IN COMPLEX WITH ANTIGENIC PEPTIDE

Structural highlights

1f90 is a 3 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.6Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The three-dimensional structure of the Fab fragment of a monoclonal antibody (LNKB-2) to human interleukin-2 (IL-2) complexed with a synthetic antigenic nonapeptide, Ac-Lys-Pro-Leu-Glu-Glu-Val-Leu-Asn-Leu-OMe, has been determined at 3.0 A resolution. In the structure, four out of the six hypervariable loops of the Fab (complementarity determining regions [CDRs] L1, H1, H2, and H3) are involved in peptide association through hydrogen bonding, salt bridge formation, and hydrophobic interactions. The Tyr residues in the Fab antigen binding site play a major role in antigen-antibody recognition. The structures of the complexed and uncomplexed Fab were compared. In the antigen binding site the CDR-L1 loop of the antibody shows the largest structural changes upon peptide binding. The peptide adopts a mostly alpha-helical conformation similar to that in the epitope fragment 64-72 of the IL-2 antigen. The side chains of residues Leu 66, Val 69, and Leu 70, which are shielded internally in the IL-2 structure, are involved in interactions with the Fab in the complex studied. This indicates that antibody-antigen complexation involves a significant rearrangement of the epitope-containing region of the IL-2 with retention of the alpha-helical character of the epitope fragment.

Crystal structure of an anti-interleukin-2 monoclonal antibody Fab complexed with an antigenic nonapeptide.,Afonin PV, Fokin AV, Tsygannik IN, Mikhailova IY, Onoprienko LV, Mikhaleva II, Ivanov VT, Mareeva TY, Nesmeyanov VA, Li N, Pangborn WA, Duax WL, Pletnev VZ Protein Sci. 2001 Aug;10(8):1514-21. PMID:11468348^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Afonin PV, Fokin AV, Tsygannik IN, Mikhailova IY, Onoprienko LV, Mikhaleva II, Ivanov VT, Mareeva TY, Nesmeyanov VA, Li N, Pangborn WA, Duax WL, Pletnev VZ. Crystal structure of an anti-interleukin-2 monoclonal antibody Fab complexed with an antigenic nonapeptide. Protein Sci. 2001 Aug;10(8):1514-21. PMID:11468348

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1f90"

@@ Line 3: / Line 3: @@
 <StructureSection load='1f90' size='340' side='right'caption='[[1f90]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1f90]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F90 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F90 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1f90]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F90 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F90 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f90 OCA], [http://pdbe.org/1f90 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1f90 RCSB], [http://www.ebi.ac.uk/pdbsum/1f90 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1f90 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f90 OCA], [https://pdbe.org/1f90 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f90 RCSB], [https://www.ebi.ac.uk/pdbsum/1f90 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f90 ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 11: / Line 12: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f9/1f90_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 34: / Line 35: @@
 [[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Afonin, P V]]
+[[Category: Afonin PV]]
-[[Category: Duax, W L]]
+[[Category: Duax WL]]
-[[Category: Fokin, A V]]
+[[Category: Fokin AV]]
-[[Category: Ivanov, V T]]
+[[Category: Ivanov VT]]
-[[Category: Li, N]]
+[[Category: Li N]]
-[[Category: Mareeva, T Y]]
+[[Category: Mareeva TY]]
-[[Category: Mikhailova, I Y]]
+[[Category: Mikhailova IY]]
-[[Category: Mikhaleva, I I]]
+[[Category: Mikhaleva II]]
-[[Category: Nesmeyanov, V A]]
+[[Category: Nesmeyanov VA]]
-[[Category: Onoprienko, L V]]
+[[Category: Onoprienko LV]]
-[[Category: Pletnev, V Z]]
+[[Category: Pletnev VZ]]
-[[Category: Tsigannik, I N]]
+[[Category: Tsigannik IN]]
-[[Category: Antigen-binding fragment]]
-[[Category: Antigenic peptide]]
-[[Category: Immune system]]
-[[Category: Interleukin-2]]
-[[Category: Monoclonal antibody]]

1f90

From Proteopedia

Current revision

FAB FRAGMENT OF MONOCLONAL ANTIBODY (LNKB-2) AGAINST HUMAN INTERLEUKIN-2 IN COMPLEX WITH ANTIGENIC PEPTIDE

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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