1jtd

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "1jtd" [edit=sysop:move=sysop])
Current revision (06:51, 30 October 2024) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1jtd.png|left|200px]]
 
-
{{STRUCTURE_1jtd| PDB=1jtd | SCENE= }}
+
==Crystal structure of beta-lactamase inhibitor protein-II in complex with TEM-1 beta-lactamase==
 +
<StructureSection load='1jtd' size='340' side='right'caption='[[1jtd]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1jtd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JTD FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jtd OCA], [https://pdbe.org/1jtd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jtd RCSB], [https://www.ebi.ac.uk/pdbsum/1jtd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jtd ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/BLAT_ECOLX BLAT_ECOLX] TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/1jtd_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jtd ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The structure of the 28 kDa beta-lactamase inhibitor protein-II (BLIP-II) in complex with the TEM-1 beta-lactamase has been determined to 2.3 A resolution. BLIP-II is a secreted protein produced by the soil bacterium Streptomyces exfoliatus SMF19 and is able to bind and inhibit TEM-1 with subnanomolar affinity. BLIP-II is a seven-bladed beta-propeller with a unique blade motif consisting of only three antiparallel beta-strands. The overall fold is highly similar to the core structure of the human regulator of chromosome condensation (RCC1). Although BLIP-II does not share the same fold with BLIP, the first beta-lactamase inhibitor protein for which structural data was available, a comparison of the two complexes reveals a number of similarities and provides further insights into key components of the TEM-1-BLIP and TEM-1-BLIP-II interfaces. Our preliminary results from gene knock-out studies and scanning electron microscopy also reveal a critical role of BLIP-II in sporulation.
-
===Crystal structure of beta-lactamase inhibitor protein-II in complex with TEM-1 beta-lactamase===
+
Crystal structure and kinetic analysis of beta-lactamase inhibitor protein-II in complex with TEM-1 beta-lactamase.,Lim D, Park HU, De Castro L, Kang SG, Lee HS, Jensen S, Lee KJ, Strynadka NC Nat Struct Biol. 2001 Oct;8(10):848-52. PMID:11573088<ref>PMID:11573088</ref>
-
{{ABSTRACT_PUBMED_11573088}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 1jtd" style="background-color:#fffaf0;"></div>
-
[[1jtd]] is a 2 chain structure of [[Beta-lactamase]] and [[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JTD OCA].
+
==See Also==
==See Also==
-
*[[Beta-lactamase|Beta-lactamase]]
+
*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]
*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:011573088</ref><references group="xtra"/>
+
__TOC__
-
[[Category: Beta-lactamase]]
+
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
 +
[[Category: Large Structures]]
[[Category: Streptomyces exfoliatus]]
[[Category: Streptomyces exfoliatus]]
-
[[Category: Castro, L De.]]
+
[[Category: De Castro L]]
-
[[Category: Jensen, S.]]
+
[[Category: Jensen S]]
-
[[Category: Kang, S G.]]
+
[[Category: Kang SG]]
-
[[Category: Lee, H S.]]
+
[[Category: Lee HS]]
-
[[Category: Lee, K J.]]
+
[[Category: Lee KJ]]
-
[[Category: Lim, D C.]]
+
[[Category: Lim DC]]
-
[[Category: Park, H U.]]
+
[[Category: Park HU]]
-
[[Category: Strynadka, N C.J.]]
+
[[Category: Strynadka NCJ]]
-
[[Category: Beta-lactamase inhibitor protein-ii]]
+
-
[[Category: Blip-ii]]
+
-
[[Category: Hydrolase-inhibitor complex]]
+
-
[[Category: Protein-protein complex]]
+
-
[[Category: Tem-1 beta-lactamase]]
+

Current revision

Crystal structure of beta-lactamase inhibitor protein-II in complex with TEM-1 beta-lactamase

PDB ID 1jtd

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jtd"
Personal tools