1mgr

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1mgr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mgr, resolution 1.70&Aring;" /> '''Crystal structure of...)
Current revision (07:01, 30 October 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1mgr.gif|left|200px]]<br /><applet load="1mgr" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1mgr, resolution 1.70&Aring;" />
 
-
'''Crystal structure of RNase Sa3,cytotoxic microbial ribonuclease'''<br />
 
-
==Overview==
+
==Crystal structure of RNase Sa3,cytotoxic microbial ribonuclease==
-
Ribonuclease (RNase) Sa3 is secreted by the Gram-positive bacterium, Streptomyces aureofaciens. The enzyme catalyzes the cleavage of RNA on the, 3' side of guanosine residues. Here, x-ray diffraction analysis was used, to determine the three-dimensional structure of two distinct crystalline, forms of RNase Sa3 to a resolution of 2.0 and 1.7 A. These two structures, are similar to each other as well as to that of a homolog, RNase Sa. All, of the key active-site residues of RNase Sa (Asn(42), Glu(44), Glu(57), Arg(72), and His(88)) are located in the putative active site of RNase, Sa3. Also herein, RNase Sa3 is shown to be toxic to human erythroleukemia, cells in culture. Like onconase, which is an amphibian ribonuclease in, Phase III clinical trials as a cancer chemotherapeutic, RNase Sa3 is not, inhibited by the cytosolic ribonuclease inhibitor protein. Thus, a, prokaryotic ribonuclease can be toxic to mammalian cells.
+
<StructureSection load='1mgr' size='340' side='right'caption='[[1mgr]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1mgr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kitasatospora_aureofaciens Kitasatospora aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MGR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MGR FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mgr OCA], [https://pdbe.org/1mgr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mgr RCSB], [https://www.ebi.ac.uk/pdbsum/1mgr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mgr ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RNS3_KITAU RNS3_KITAU]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mg/1mgr_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mgr ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Ribonuclease (RNase) Sa3 is secreted by the Gram-positive bacterium Streptomyces aureofaciens. The enzyme catalyzes the cleavage of RNA on the 3' side of guanosine residues. Here, x-ray diffraction analysis was used to determine the three-dimensional structure of two distinct crystalline forms of RNase Sa3 to a resolution of 2.0 and 1.7 A. These two structures are similar to each other as well as to that of a homolog, RNase Sa. All of the key active-site residues of RNase Sa (Asn(42), Glu(44), Glu(57), Arg(72), and His(88)) are located in the putative active site of RNase Sa3. Also herein, RNase Sa3 is shown to be toxic to human erythroleukemia cells in culture. Like onconase, which is an amphibian ribonuclease in Phase III clinical trials as a cancer chemotherapeutic, RNase Sa3 is not inhibited by the cytosolic ribonuclease inhibitor protein. Thus, a prokaryotic ribonuclease can be toxic to mammalian cells.
-
==About this Structure==
+
X-ray structure of two crystalline forms of a streptomycete ribonuclease with cytotoxic activity.,Sevcik J, Urbanikova L, Leland PA, Raines RT J Biol Chem. 2002 Dec 6;277(49):47325-30. Epub 2002 Sep 11. PMID:12228255<ref>PMID:12228255</ref>
-
1MGR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MGR OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
X-ray structure of two crystalline forms of a streptomycete ribonuclease with cytotoxic activity., Sevcik J, Urbanikova L, Leland PA, Raines RT, J Biol Chem. 2002 Dec 6;277(49):47325-30. Epub 2002 Sep 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12228255 12228255]
+
</div>
-
[[Category: Ribonuclease T(1)]]
+
<div class="pdbe-citations 1mgr" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
-
[[Category: Streptomyces aureofaciens]]
+
-
[[Category: Leland, P.A.]]
+
-
[[Category: Raines, R.T.]]
+
-
[[Category: Sevcik, J.]]
+
-
[[Category: Urbanikova, L.]]
+
-
[[Category: SO4]]
+
-
[[Category: alpha/beta protein]]
+
-
[[Category: ub rolls]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:24:23 2007''
+
==See Also==
 +
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Kitasatospora aureofaciens]]
 +
[[Category: Large Structures]]
 +
[[Category: Leland PA]]
 +
[[Category: Raines RT]]
 +
[[Category: Sevcik J]]
 +
[[Category: Urbanikova L]]

Current revision

Crystal structure of RNase Sa3,cytotoxic microbial ribonuclease

PDB ID 1mgr

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mgr"
Personal tools