1mgr

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[[Image:1mgr.png|left|200px]]
 
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==Crystal structure of RNase Sa3,cytotoxic microbial ribonuclease==
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The line below this paragraph, containing "STRUCTURE_1mgr", creates the "Structure Box" on the page.
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<StructureSection load='1mgr' size='340' side='right'caption='[[1mgr]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1mgr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kitasatospora_aureofaciens Kitasatospora aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MGR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MGR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1mgr| PDB=1mgr | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mgr OCA], [https://pdbe.org/1mgr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mgr RCSB], [https://www.ebi.ac.uk/pdbsum/1mgr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mgr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNS3_KITAU RNS3_KITAU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mg/1mgr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mgr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ribonuclease (RNase) Sa3 is secreted by the Gram-positive bacterium Streptomyces aureofaciens. The enzyme catalyzes the cleavage of RNA on the 3' side of guanosine residues. Here, x-ray diffraction analysis was used to determine the three-dimensional structure of two distinct crystalline forms of RNase Sa3 to a resolution of 2.0 and 1.7 A. These two structures are similar to each other as well as to that of a homolog, RNase Sa. All of the key active-site residues of RNase Sa (Asn(42), Glu(44), Glu(57), Arg(72), and His(88)) are located in the putative active site of RNase Sa3. Also herein, RNase Sa3 is shown to be toxic to human erythroleukemia cells in culture. Like onconase, which is an amphibian ribonuclease in Phase III clinical trials as a cancer chemotherapeutic, RNase Sa3 is not inhibited by the cytosolic ribonuclease inhibitor protein. Thus, a prokaryotic ribonuclease can be toxic to mammalian cells.
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===Crystal structure of RNase Sa3,cytotoxic microbial ribonuclease===
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X-ray structure of two crystalline forms of a streptomycete ribonuclease with cytotoxic activity.,Sevcik J, Urbanikova L, Leland PA, Raines RT J Biol Chem. 2002 Dec 6;277(49):47325-30. Epub 2002 Sep 11. PMID:12228255<ref>PMID:12228255</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1mgr" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12228255}}, adds the Publication Abstract to the page
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12228255 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12228255}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Kitasatospora aureofaciens]]
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1MGR is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MGR OCA].
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[[Category: Large Structures]]
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[[Category: Leland PA]]
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==Reference==
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[[Category: Raines RT]]
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<ref group="xtra">PMID:12228255</ref><references group="xtra"/>
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[[Category: Sevcik J]]
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[[Category: Streptomyces aureofaciens]]
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[[Category: Urbanikova L]]
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[[Category: Leland, P A.]]
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[[Category: Raines, R T.]]
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[[Category: Sevcik, J.]]
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[[Category: Urbanikova, L.]]
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[[Category: Alpha/beta protein]]
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[[Category: Ub roll]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 12:52:30 2009''
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Current revision

Crystal structure of RNase Sa3,cytotoxic microbial ribonuclease

PDB ID 1mgr

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