1nns

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1nns" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nns, resolution 1.95&Aring;" /> '''L-asparaginase of E....)
Current revision (07:05, 30 October 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1nns.gif|left|200px]]<br /><applet load="1nns" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1nns, resolution 1.95&Aring;" />
 
-
'''L-asparaginase of E. coli in C2 space group and 1.95 A resolution'''<br />
 
-
==Overview==
+
==L-asparaginase of E. coli in C2 space group and 1.95 A resolution==
-
The functional L-asparaginase from Escherichia coli is a homotetramer with, a molecular weight of about 142 kDa. The X-ray structure of the enzyme, crystallized in a new form (space group C2) and refined to 1.95 A, resolution, is compared with that of the previously determined crystal, form (space group P2(1)). The asymmetric unit of the new crystal form, contains an L-asparaginase dimer instead of the tetramer found in the, previous crystal form. It is found that crystal contacts practically do, not affect the conformation of the protein. It is shown that subunit C of, the tetrameric form is in a conformation which is systematically different, from that of all other subunits in both crystal forms. Major, conformational differences are confined to the lid loop (residues 14-27)., In addition, the stability of this globular protein is analyzed in terms, of the interactions between hydrophobic parts of the subunits.
+
<StructureSection load='1nns' size='340' side='right'caption='[[1nns]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1nns]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NNS FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nns OCA], [https://pdbe.org/1nns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nns RCSB], [https://www.ebi.ac.uk/pdbsum/1nns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nns ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ASPG2_ECOLI ASPG2_ECOLI]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nn/1nns_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nns ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The functional L-asparaginase from Escherichia coli is a homotetramer with a molecular weight of about 142 kDa. The X-ray structure of the enzyme, crystallized in a new form (space group C2) and refined to 1.95 A resolution, is compared with that of the previously determined crystal form (space group P2(1)). The asymmetric unit of the new crystal form contains an L-asparaginase dimer instead of the tetramer found in the previous crystal form. It is found that crystal contacts practically do not affect the conformation of the protein. It is shown that subunit C of the tetrameric form is in a conformation which is systematically different from that of all other subunits in both crystal forms. Major conformational differences are confined to the lid loop (residues 14-27). In addition, the stability of this globular protein is analyzed in terms of the interactions between hydrophobic parts of the subunits.
-
==About this Structure==
+
Structural comparison of Escherichia coli L-asparaginase in two monoclinic space groups.,Sanches M, Barbosa JA, de Oliveira RT, Abrahao Neto J, Polikarpov I Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):416-22. Epub 2003, Feb 21. PMID:12595697<ref>PMID:12595697</ref>
-
1NNS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ASP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NNS OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Structural comparison of Escherichia coli L-asparaginase in two monoclinic space groups., Sanches M, Barbosa JA, de Oliveira RT, Abrahao Neto J, Polikarpov I, Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):416-22. Epub 2003, Feb 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12595697 12595697]
+
</div>
-
[[Category: Asparaginase]]
+
<div class="pdbe-citations 1nns" style="background-color:#fffaf0;"></div>
-
[[Category: Escherichia coli]]
+
-
[[Category: Single protein]]
+
-
[[Category: Barbosa, J.A.R.G.]]
+
-
[[Category: Neto, J.A.A.]]
+
-
[[Category: Oliveira, R.T.de.]]
+
-
[[Category: Polikarpov, I.]]
+
-
[[Category: Sanches, M.]]
+
-
[[Category: ASP]]
+
-
[[Category: amidrohydrolase]]
+
-
[[Category: crystallographic comparison]]
+
-
[[Category: l-asparaginase]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:24:56 2007''
+
==See Also==
 +
*[[Asparaginase 3D structures|Asparaginase 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Escherichia coli]]
 +
[[Category: Large Structures]]
 +
[[Category: Barbosa JARG]]
 +
[[Category: Neto JAA]]
 +
[[Category: Polikarpov I]]
 +
[[Category: Sanches M]]
 +
[[Category: De Oliveira RT]]

Current revision

L-asparaginase of E. coli in C2 space group and 1.95 A resolution

PDB ID 1nns

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nns"
Personal tools