1u3h

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[[Image:1u3h.gif|left|200px]]
 
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{{Structure
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==Crystal structure of mouse TCR 172.10 complexed with MHC class II I-Au molecule at 2.4 A==
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|PDB= 1u3h |SIZE=350|CAPTION= <scene name='initialview01'>1u3h</scene>, resolution 2.42&Aring;
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<StructureSection load='1u3h' size='340' side='right'caption='[[1u3h]], [[Resolution|resolution]] 2.42&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1u3h]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U3H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U3H FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.42&#8491;</td></tr>
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|GENE= H2-Aa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u3h OCA], [https://pdbe.org/1u3h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u3h RCSB], [https://www.ebi.ac.uk/pdbsum/1u3h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u3h ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q5R1B3_MOUSE Q5R1B3_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u3/1u3h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u3h ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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T cell receptor crossreactivity with different peptide ligands and biased recognition of MHC are coupled features of antigen recognition that are necessary for the T cell's diverse functional repertoire. In the crystal structure between an autoreactive, EAE T cell clone 172.10 and myelin basic protein (1-11) presented by class II MHC I-Au, recognition of the MHC is dominated by the Vbeta domain of the TCR, which interacts with the MHC alpha chain in a manner suggestive of a germline-encoded TCR/MHC "anchor point." Strikingly, there are few specific contacts between the TCR CDR3 loops and the MBP peptide. We also find that over 1,000,000 different peptides derived from combinatorial libraries can activate 172.10, yet the TCR strongly prefers the native MBP contact residues. We suggest that while TCR scanning of pMHC may be degenerate due to the TCR germline bias for MHC, recognition of structurally distinct agonist peptides is not indicative of TCR promiscuity, but rather highly specific alternative solutions to TCR engagement.
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'''Crystal structure of mouse TCR 172.10 complexed with MHC class II I-Au molecule at 2.4 A'''
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Structure of an autoimmune T cell receptor complexed with class II peptide-MHC: insights into MHC bias and antigen specificity.,Maynard J, Petersson K, Wilson DH, Adams EJ, Blondelle SE, Boulanger MJ, Wilson DB, Garcia KC Immunity. 2005 Jan;22(1):81-92. PMID:15664161<ref>PMID:15664161</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1u3h" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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T cell receptor crossreactivity with different peptide ligands and biased recognition of MHC are coupled features of antigen recognition that are necessary for the T cell's diverse functional repertoire. In the crystal structure between an autoreactive, EAE T cell clone 172.10 and myelin basic protein (1-11) presented by class II MHC I-Au, recognition of the MHC is dominated by the Vbeta domain of the TCR, which interacts with the MHC alpha chain in a manner suggestive of a germline-encoded TCR/MHC "anchor point." Strikingly, there are few specific contacts between the TCR CDR3 loops and the MBP peptide. We also find that over 1,000,000 different peptides derived from combinatorial libraries can activate 172.10, yet the TCR strongly prefers the native MBP contact residues. We suggest that while TCR scanning of pMHC may be degenerate due to the TCR germline bias for MHC, recognition of structurally distinct agonist peptides is not indicative of TCR promiscuity, but rather highly specific alternative solutions to TCR engagement.
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*[[MHC 3D structures|MHC 3D structures]]
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*[[MHC II 3D structures|MHC II 3D structures]]
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==About this Structure==
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*[[T-cell receptor 3D structures|T-cell receptor 3D structures]]
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1U3H is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U3H OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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Structure of an autoimmune T cell receptor complexed with class II peptide-MHC: insights into MHC bias and antigen specificity., Maynard J, Petersson K, Wilson DH, Adams EJ, Blondelle SE, Boulanger MJ, Wilson DB, Garcia KC, Immunity. 2005 Jan;22(1):81-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15664161 15664161]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Synthetic construct]]
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[[Category: Adams, E J.]]
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[[Category: Adams EJ]]
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[[Category: Blondelle, S E.]]
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[[Category: Blondelle SE]]
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[[Category: Boulanger, M J.]]
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[[Category: Boulanger MJ]]
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[[Category: Garcia, K C.]]
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[[Category: Garcia KC]]
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[[Category: Maynard, J.]]
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[[Category: Maynard J]]
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[[Category: Petersson, K.]]
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[[Category: Petersson K]]
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[[Category: Wilson, D B.]]
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[[Category: Wilson DB]]
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[[Category: Wilson, D H.]]
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[[Category: Wilson DH]]
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[[Category: complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:26:55 2008''
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Current revision

Crystal structure of mouse TCR 172.10 complexed with MHC class II I-Au molecule at 2.4 A

PDB ID 1u3h

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