1z19

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(New page: 200px<br /><applet load="1z19" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z19, resolution 2.8&Aring;" /> '''Crystal structure of ...)
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[[Image:1z19.gif|left|200px]]<br /><applet load="1z19" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1z19, resolution 2.8&Aring;" />
 
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'''Crystal structure of a lambda integrase(75-356) dimer bound to a COC' core site'''<br />
 
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==Overview==
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==Crystal structure of a lambda integrase(75-356) dimer bound to a COC' core site==
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Site-specific DNA recombination is important for basic cellular functions, including viral integration, control of gene expression, production of, genetic diversity and segregation of newly replicated chromosomes, and is, used by bacteriophage lambda to integrate or excise its genome into and, out of the host chromosome. lambda recombination is carried out by the, bacteriophage-encoded integrase protein (lambda-int) together with, accessory DNA sites and associated bending proteins that allow regulation, in response to cell physiology. Here we report the crystal structures of, lambda-int in higher-order complexes with substrates and regulatory DNAs, representing different intermediates along the reaction pathway. The, structures show how the simultaneous binding of two separate domains of, lambda-int to DNA facilitates synapsis and can specify the order of DNA, strand cleavage and exchange. An intertwined layer of amino-terminal, domains bound to accessory (arm) DNAs shapes the recombination complex in, a way that suggests how arm binding shifts the reaction equilibrium in, favour of recombinant products.
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<StructureSection load='1z19' size='340' side='right'caption='[[1z19]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1z19]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z19 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z19 OCA], [https://pdbe.org/1z19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z19 RCSB], [https://www.ebi.ac.uk/pdbsum/1z19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z19 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VINT_LAMBD VINT_LAMBD] Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z1/1z19_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z19 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Site-specific DNA recombination is important for basic cellular functions including viral integration, control of gene expression, production of genetic diversity and segregation of newly replicated chromosomes, and is used by bacteriophage lambda to integrate or excise its genome into and out of the host chromosome. lambda recombination is carried out by the bacteriophage-encoded integrase protein (lambda-int) together with accessory DNA sites and associated bending proteins that allow regulation in response to cell physiology. Here we report the crystal structures of lambda-int in higher-order complexes with substrates and regulatory DNAs representing different intermediates along the reaction pathway. The structures show how the simultaneous binding of two separate domains of lambda-int to DNA facilitates synapsis and can specify the order of DNA strand cleavage and exchange. An intertwined layer of amino-terminal domains bound to accessory (arm) DNAs shapes the recombination complex in a way that suggests how arm binding shifts the reaction equilibrium in favour of recombinant products.
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==About this Structure==
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A structural basis for allosteric control of DNA recombination by lambda integrase.,Biswas T, Aihara H, Radman-Livaja M, Filman D, Landy A, Ellenberger T Nature. 2005 Jun 23;435(7045):1059-66. PMID:15973401<ref>PMID:15973401</ref>
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1Z19 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z19 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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A structural basis for allosteric control of DNA recombination by lambda integrase., Biswas T, Aihara H, Radman-Livaja M, Filman D, Landy A, Ellenberger T, Nature. 2005 Jun 23;435(7045):1059-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15973401 15973401]
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</div>
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[[Category: Enterobacteria phage lambda]]
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<div class="pdbe-citations 1z19" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Aihara, H.]]
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[[Category: Biswas, T.]]
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[[Category: Ellenberger, T.]]
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[[Category: Filman, D.]]
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[[Category: Landy, A.]]
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[[Category: Radman-Livaja, M.]]
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[[Category: protein-dna complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:11:11 2007''
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==See Also==
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*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia virus Lambda]]
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[[Category: Large Structures]]
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[[Category: Aihara H]]
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[[Category: Biswas T]]
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[[Category: Ellenberger T]]
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[[Category: Filman D]]
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[[Category: Landy A]]
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[[Category: Radman-Livaja M]]

Current revision

Crystal structure of a lambda integrase(75-356) dimer bound to a COC' core site

PDB ID 1z19

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