1txq
From Proteopedia
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'''Crystal structure of the EB1 C-terminal domain complexed with the CAP-Gly domain of p150Glued''' | '''Crystal structure of the EB1 C-terminal domain complexed with the CAP-Gly domain of p150Glued''' | ||
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[[Category: Hayashi, I.]] | [[Category: Hayashi, I.]] | ||
[[Category: Ikura, M.]] | [[Category: Ikura, M.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:29:51 2008'' | |
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Revision as of 07:29, 3 May 2008
Crystal structure of the EB1 C-terminal domain complexed with the CAP-Gly domain of p150Glued
Overview
Plus-end tracking proteins, such as EB1 and the dynein/dynactin complex, regulate microtubule dynamics. These proteins are thought to stabilize microtubules by forming a plus-end complex at microtubule growing ends with ill-defined mechanisms. Here we report the crystal structure of two plus-end complex components, the carboxy-terminal dimerization domain of EB1 and the microtubule binding (CAP-Gly) domain of the dynactin subunit p150Glued. Each molecule of the EB1 dimer contains two helices forming a conserved four-helix bundle, while also providing p150Glued binding sites in its flexible tail region. Combining crystallography, NMR, and mutational analyses, our studies reveal the critical interacting elements of both EB1 and p150Glued, whose mutation alters microtubule polymerization activity. Moreover, removal of the key flexible tail from EB1 activates microtubule assembly by EB1 alone, suggesting that the flexible tail negatively regulates EB1 activity. We, therefore, propose that EB1 possesses an auto-inhibited conformation, which is relieved by p150Glued as an allosteric activator.
About this Structure
1TXQ is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis for the activation of microtubule assembly by the EB1 and p150Glued complex., Hayashi I, Wilde A, Mal TK, Ikura M, Mol Cell. 2005 Aug 19;19(4):449-60. PMID:16109370 Page seeded by OCA on Sat May 3 10:29:51 2008
