2igr

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{{Seed}}
 
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[[Image:2igr.png|left|200px]]
 
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==Solution structure of CB1a, a novel anticancer peptide derived from natural antimicrobial peptide cecropin B==
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The line below this paragraph, containing "STRUCTURE_2igr", creates the "Structure Box" on the page.
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<StructureSection load='2igr' size='340' side='right'caption='[[2igr]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2igr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hyalophora_cecropia Hyalophora cecropia]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IGR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IGR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
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{{STRUCTURE_2igr| PDB=2igr | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2igr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2igr OCA], [https://pdbe.org/2igr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2igr RCSB], [https://www.ebi.ac.uk/pdbsum/2igr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2igr ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Several natural antimicrobial peptides including cecropins, magainins and melittins have been found to kill cancer cells. However, their efficacy may not be adequate for their development as anticancer agents. In this study, we used a natural antimicrobial peptide, cecropin B (CB), as a template to generate a novel anticancer peptide. Cecropin B is an amphipathic and polycationic peptide derived from the hemolymph of Hyalophora cecropia with well-known antimicrobial and cytolytic properties. The signature pattern of cecropins is W-x-(0,2)-[KDN]-x-{L}-K-[KRE]-[LI]-E-[RKN] (PROSITE: PS00268), and this signature sequence is located at N-terminus of CB. CB1a was constructed by repeating the N-terminal ten amino acids of CB three times and including a hinge near C-terminus. The circular dichroism spectra showed that CB1a is unstructured in aqueous solution, but adopt a helical conformation in membrane-like environment. The solution structure of CB1a in a polar solvent was also studied by NMR. CB1a formed a helix-hinge-helix in 20% HFIP solution, and it was found the bent angle between two helical segments was induced ranging from 60 degrees to 110 degrees . A heparin-binding motif is located in the central part of helix 1. Isothermal titration calorimetry reveals the association constant of CB1a bound to low molecular weight heparin is 1.66 x 10(5)M(-1) at physiological ionic strength at 25 degrees C. Binding of CB1a to heparin produces a large conformational change toward a more structural state. CB1a demonstrated promising activity against several cancer cells but low toxicity against non-cancer cells. The IC(50) of CB1a on leukemia and stomach carcinoma cells were in the range of 2-8-fold lower than those of CB. Besides, CB1a exhibited low hemolytic activity against human red blood cells. Due to these properties, CB1a has the potential to become a promising anticancer agent.
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===Solution structure of CB1a, a novel anticancer peptide derived from natural antimicrobial peptide cecropin B===
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Structure and function of a custom anticancer peptide, CB1a.,Wu JM, Jan PS, Yu HC, Haung HY, Fang HJ, Chang YI, Cheng JW, Chen HM Peptides. 2009 May;30(5):839-48. Epub 2009 Feb 13. PMID:19428759<ref>PMID:19428759</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19428759}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2igr" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19428759 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19428759}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Hyalophora cecropia]]
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2IGR is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IGR OCA].
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[[Category: Large Structures]]
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[[Category: Wu J-M]]
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==Reference==
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<ref group="xtra">PMID:19428759</ref><references group="xtra"/>
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[[Category: Wu, J M.]]
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[[Category: Anticancer peptide]]
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[[Category: Antimicrobial peptide]]
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[[Category: Cecropin]]
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[[Category: Cecropin fingerprint sequence]]
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[[Category: De novo protein]]
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[[Category: Lipid binding protein]]
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[[Category: Ploycationic peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 1 11:04:47 2010''
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Current revision

Solution structure of CB1a, a novel anticancer peptide derived from natural antimicrobial peptide cecropin B

PDB ID 2igr

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