2mp1

From Proteopedia

(Difference between revisions)

Current revision

Solution structure of the human chemokine CCL19

Structural highlights

2mp1 is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 20 models
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CCL19_HUMAN May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.^[1]

Publication Abstract from PubMed

CCL19 and CCL21 are chemokines involved in the trafficking of immune cells, particularly within the lymphatic system, through activation of CCR7. Concurrent expression of PSGL-1 and CCR7 in naive T-cells enhances recruitment of these cells to secondary lymphoid organs by CCL19 and CCL21. Here the solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. Implications for the mechanism of PSGL-1's enhancement of resting T-cell recruitment are discussed.

Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites.,Veldkamp CT, Kiermaier E, Gabel-Eissens SJ, Gillitzer ML, Lippner DR, DiSilvio FA, Mueller CJ, Wantuch PL, Chaffee GR, Famiglietti MW, Zgoba DM, Bailey AA, Bah Y, Engebretson SJ, Graupner DR, Lackner ER, LaRosa VD, Medeiros T, Olson ML, Phillips AJ, Pyles H, Richard AM, Schoeller SJ, Touzeau B, Williams LG, Sixt M, Peterson FC Biochemistry. 2015 Jul 14;54(27):4163-6. doi: 10.1021/acs.biochem.5b00560. Epub, 2015 Jul 6. PMID:26115234^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kim CH, Pelus LM, White JR, Applebaum E, Johanson K, Broxmeyer HE. CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells. J Immunol. 1998 Mar 1;160(5):2418-24. PMID:9498785
↑ Veldkamp CT, Kiermaier E, Gabel-Eissens SJ, Gillitzer ML, Lippner DR, DiSilvio FA, Mueller CJ, Wantuch PL, Chaffee GR, Famiglietti MW, Zgoba DM, Bailey AA, Bah Y, Engebretson SJ, Graupner DR, Lackner ER, LaRosa VD, Medeiros T, Olson ML, Phillips AJ, Pyles H, Richard AM, Schoeller SJ, Touzeau B, Williams LG, Sixt M, Peterson FC. Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites. Biochemistry. 2015 Jul 14;54(27):4163-6. doi: 10.1021/acs.biochem.5b00560. Epub, 2015 Jul 6. PMID:26115234 doi:http://dx.doi.org/10.1021/acs.biochem.5b00560

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2mp1"

@@ Line 1: / Line 1: @@
 ==Solution structure of the human chemokine CCL19==
-<StructureSection load='2mp1' size='340' side='right'caption='[[2mp1]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2mp1' size='340' side='right'caption='[[2mp1]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2mp1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MP1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2mp1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MP1 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CCL19, ELC, MIP3B, SCYA19 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mp1 OCA], [https://pdbe.org/2mp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mp1 RCSB], [https://www.ebi.ac.uk/pdbsum/2mp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mp1 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/CCL19_HUMAN CCL19_HUMAN]] May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.<ref>PMID:9498785</ref>
+[https://www.uniprot.org/uniprot/CCL19_HUMAN CCL19_HUMAN] May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.<ref>PMID:9498785</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Gabel-Eissens, S J]]
+[[Category: Gabel-Eissens SJ]]
-[[Category: Gillitzer, M L]]
+[[Category: Gillitzer ML]]
-[[Category: Peterson, F C]]
+[[Category: Peterson FC]]
-[[Category: Veldkamp, C T]]
+[[Category: Veldkamp CT]]
-[[Category: Ccl19]]
-[[Category: Ccl21]]
-[[Category: Ccr7]]
-[[Category: Chemokine]]
-[[Category: Chemokine receptor]]
-[[Category: Signaling protein]]

2mp1

From Proteopedia

Current revision

Solution structure of the human chemokine CCL19

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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