2ns8

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(New page: 200px<br /><applet load="2ns8" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ns8, resolution 2.550&Aring;" /> '''How an in vitro sel...)
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[[Image:2ns8.jpg|left|200px]]<br /><applet load="2ns8" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ns8, resolution 2.550&Aring;" />
 
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'''How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor'''<br />
 
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==Overview==
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==How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor==
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A 16-residue peptide, called Tip, induces the tetracycline repressor TetR, as efficiently as the antibiotic tetracycline when fused to the N or C, terminus of another protein. This is unusual because the majority of in, vitro selected peptides, such as Tip, inhibit protein function, and, agonist peptides are only rarely identified. We elucidated the atomic, mechanism of TetR induction by Tip from crystal structures of TetR in, complex with Tip and of free TetR. Peptide induction ultimately results in, the same movements of DNA reading heads, but Tip accomplishes this by very, different molecular interactions from tetracycline involving important, contacts to the TetR surface. As a direct consequence, an alternate, pathway of allostery becomes possible that makes ample use of intersubunit, interactions. For the first time it is possible to show in atomic detail, how a small molecule controlled bacterial transcription factor such as, TetR becomes responsive to protein-protein interactions, characteristic of, gene transcription regulation in higher organisms.
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<StructureSection load='2ns8' size='340' side='right'caption='[[2ns8]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ns8]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NS8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ns8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ns8 OCA], [https://pdbe.org/2ns8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ns8 RCSB], [https://www.ebi.ac.uk/pdbsum/2ns8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ns8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ns/2ns8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ns8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A 16-residue peptide, called Tip, induces the tetracycline repressor TetR as efficiently as the antibiotic tetracycline when fused to the N or C terminus of another protein. This is unusual because the majority of in vitro selected peptides, such as Tip, inhibit protein function, and agonist peptides are only rarely identified. We elucidated the atomic mechanism of TetR induction by Tip from crystal structures of TetR in complex with Tip and of free TetR. Peptide induction ultimately results in the same movements of DNA reading heads, but Tip accomplishes this by very different molecular interactions from tetracycline involving important contacts to the TetR surface. As a direct consequence, an alternate pathway of allostery becomes possible that makes ample use of intersubunit interactions. For the first time it is possible to show in atomic detail how a small molecule controlled bacterial transcription factor such as TetR becomes responsive to protein-protein interactions, characteristic of gene transcription regulation in higher organisms.
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==About this Structure==
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How an agonist peptide mimics the antibiotic tetracycline to induce Tet-repressor.,Luckner SR, Klotzsche M, Berens C, Hillen W, Muller YA J Mol Biol. 2007 May 4;368(3):780-90. Epub 2007 Feb 22. PMID:17374541<ref>PMID:17374541</ref>
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2NS8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS8 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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How an agonist peptide mimics the antibiotic tetracycline to induce Tet-repressor., Luckner SR, Klotzsche M, Berens C, Hillen W, Muller YA, J Mol Biol. 2007 May 4;368(3):780-90. Epub 2007 Feb 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17374541 17374541]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 2ns8" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Berens, C.]]
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[[Category: Hillen, W.]]
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[[Category: Klotzsche, M.]]
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[[Category: Luckner, S.R.]]
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[[Category: Muller, Y.A.]]
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[[Category: bound repressor-inducing peptide]]
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[[Category: helix-turn-helix]]
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[[Category: transcription regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:25:04 2008''
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==See Also==
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*[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
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[[Category: Berens C]]
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[[Category: Hillen W]]
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[[Category: Klotzsche M]]
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[[Category: Luckner SR]]
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[[Category: Muller YA]]

Current revision

How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor

PDB ID 2ns8

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