3n8s

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[[Image:3n8s.png|left|200px]]
 
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==Crystal Structure of BlaC-E166A covalently bound with Cefamandole==
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The line below this paragraph, containing "STRUCTURE_3n8s", creates the "Structure Box" on the page.
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<StructureSection load='3n8s' size='340' side='right'caption='[[3n8s]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3n8s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N8S FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=XD2:(2R)-2-[(1R)-1-{[(2R)-2-HYDROXY-2-PHENYLACETYL]AMINO}-2-OXOETHYL]-5-{[(1-METHYL-1H-TETRAZOL-5-YL)SULFANYL]METHYL}-3,6-DIHYDRO-2H-1,3-THIAZINE-4-CARBOXYLIC+ACID'>XD2</scene></td></tr>
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{{STRUCTURE_3n8s| PDB=3n8s | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n8s OCA], [https://pdbe.org/3n8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n8s RCSB], [https://www.ebi.ac.uk/pdbsum/3n8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n8s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BLAC_MYCTU BLAC_MYCTU]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The genome of Mycobacterium tuberculosis (TB) contains a gene that encodes a highly active beta-lactamase, BlaC, that imparts TB with resistance to beta-lactam chemotherapy. The structure of covalent BlaC-beta-lactam complexes suggests that active site residues K73 and E166 are essential for acylation and deacylation, respectively. We have prepared the K73A and E166A mutant forms of BlaC and have determined the structures of the Michaelis complex of cefamandole and the covalently bound acyl intermediate of cefamandole at resolutions of 1.2 and 2.0 A, respectively. These structures provide insight into the details of the catalytic mechanism.
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===Crystal Structure of BlaC-E166A covalently bound with Cefamandole===
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Structures of the Michaelis Complex (1.2 A) and the Covalent Acyl Intermediate (2.0 A) of Cefamandole Bound in the Active Sites of the Mycobacterium tuberculosis beta-Lactamase K73A and E166A Mutants .,Tremblay LW, Xu H, Blanchard JS Biochemistry. 2010 Nov 16;49(45):9685-7. Epub 2010 Oct 25. PMID:20961112<ref>PMID:20961112</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3n8s" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20961112 is the PubMed ID number.
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{{ABSTRACT_PUBMED_20961112}}
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==About this Structure==
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[[3n8s]] is a 1 chain structure of [[Beta-lactamase]] with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N8S OCA].
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==See Also==
==See Also==
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*[[Beta-lactamase|Beta-lactamase]]
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*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020961112</ref><references group="xtra"/>
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__TOC__
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[[Category: Beta-lactamase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Blanchard, J S.]]
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[[Category: Blanchard JS]]
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[[Category: Tremblay, L W.]]
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[[Category: Tremblay LW]]
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[[Category: Beta-lactam covalent adduct]]
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[[Category: Hydrolase-antibiotic complex]]
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[[Category: Penicillin binding protein]]
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Current revision

Crystal Structure of BlaC-E166A covalently bound with Cefamandole

PDB ID 3n8s

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