4dbl

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[[Image:4dbl.jpg|left|200px]]
 
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==Crystal structure of E159Q mutant of BtuCDF==
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The line below this paragraph, containing "STRUCTURE_4dbl", creates the "Structure Box" on the page.
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<StructureSection load='4dbl' size='340' side='right'caption='[[4dbl]], [[Resolution|resolution]] 3.49&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[4dbl]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DBL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DBL FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.493&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_4dbl| PDB=4dbl | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dbl OCA], [https://pdbe.org/4dbl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dbl RCSB], [https://www.ebi.ac.uk/pdbsum/4dbl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dbl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BTUC_ECOLI BTUC_ECOLI] Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Involved in the translocation of the substrate across the membrane.[HAMAP-Rule:MF_01004]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BtuCD is an ABC transporter catalyzing the uptake of vitamin B(12) across the Escherichia coli inner membrane. A previously reported X-ray structure of BtuCD in complex with the periplasmic vitamin B(12)-binding protein BtuF revealed asymmetry of the transmembrane BtuC subunits. The functional relevance of this asymmetry has remained uncertain. Here we report the X-ray structure of a catalytically impaired BtuCD mutant in complex with BtuF, where the BtuC subunits adopt a distinct asymmetric conformation. The structure suggests that BtuF does not discriminate between, or impose, asymmetric conformations of BtuCD. It also explains the conformational disorder observed in BtuCDF crystals.
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===Crystal structure of E159Q mutant of BtuCDF===
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Asymmetric states of vitamin B12 transporter BtuCD are not discriminated by its cognate substrate binding protein BtuF.,Korkhov VM, Mireku SA, Hvorup RN, Locher KP FEBS Lett. 2012 Apr 5;586(7):972-6. Epub 2012 Mar 8. PMID:22569249<ref>PMID:22569249</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[4dbl]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DBL OCA].
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<div class="pdbe-citations 4dbl" style="background-color:#fffaf0;"></div>
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[[Category: Escherichia coli]]
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== References ==
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[[Category: Vitamin B12-transporting ATPase]]
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<references/>
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[[Category: Hvorup, R N.]]
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__TOC__
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[[Category: Korkhov, V M.]]
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</StructureSection>
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[[Category: Locher, K P.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Mireku, S M.]]
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[[Category: Large Structures]]
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[[Category: Abc transporter for vitamin b12]]
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[[Category: Hvorup RN]]
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[[Category: Atp binding]]
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[[Category: Korkhov VM]]
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[[Category: Inner membrane]]
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[[Category: Locher KP]]
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[[Category: Transport protein]]
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[[Category: Mireku SM]]

Current revision

Crystal structure of E159Q mutant of BtuCDF

PDB ID 4dbl

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