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| | ==Crystal Structure of the TrkH/TrkA potassium transport complex== | | ==Crystal Structure of the TrkH/TrkA potassium transport complex== |
| - | <StructureSection load='4j9u' size='340' side='right' caption='[[4j9u]], [[Resolution|resolution]] 3.80Å' scene=''> | + | <StructureSection load='4j9u' size='340' side='right'caption='[[4j9u]], [[Resolution|resolution]] 3.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4j9u]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibpa Vibpa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J9U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J9U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4j9u]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_parahaemolyticus_RIMD_2210633 Vibrio parahaemolyticus RIMD 2210633]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J9U FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=TBR:HEXATANTALUM+DODECABROMIDE'>TBR</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4j9v|4j9v]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">trkH, VP0032 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=223926 VIBPA]), VP3045 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=223926 VIBPA])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j9u OCA], [https://pdbe.org/4j9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j9u RCSB], [https://www.ebi.ac.uk/pdbsum/4j9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j9u ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j9u OCA], [http://pdbe.org/4j9u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j9u RCSB], [http://www.ebi.ac.uk/pdbsum/4j9u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j9u ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TRKH_VIBPA TRKH_VIBPA]] Low-affinity potassium transport system. Interacts with trk system potassium uptake protein TrkA and requires TrkE for transport activity. Selective for permeation of potassium ion and rubidium ion over smaller ions such as natrium or litium. | + | [https://www.uniprot.org/uniprot/TRKH_VIBPA TRKH_VIBPA] Low-affinity potassium transport system. Interacts with trk system potassium uptake protein TrkA and requires TrkE for transport activity. Selective for permeation of potassium ion and rubidium ion over smaller ions such as natrium or litium. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4j9u" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4j9u" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Tyrosine kinase receptor 3D structures|Tyrosine kinase receptor 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Vibpa]] | + | [[Category: Large Structures]] |
| - | [[Category: Cao, Y]] | + | [[Category: Vibrio parahaemolyticus RIMD 2210633]] |
| - | [[Category: Huang, H]] | + | [[Category: Cao Y]] |
| - | [[Category: Jin, X]] | + | [[Category: Huang H]] |
| - | [[Category: Levin, E J]] | + | [[Category: Jin X]] |
| - | [[Category: NYCOMPS, New York Consortium on Membrane Protein Structure]]
| + | [[Category: Levin EJ]] |
| - | [[Category: Zhou, M]] | + | [[Category: Zhou M]] |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: New york consortium on membrane protein structure]]
| + | |
| - | [[Category: Nycomp]]
| + | |
| - | [[Category: Potassium transport]]
| + | |
| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Rck domain]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
TRKH_VIBPA Low-affinity potassium transport system. Interacts with trk system potassium uptake protein TrkA and requires TrkE for transport activity. Selective for permeation of potassium ion and rubidium ion over smaller ions such as natrium or litium.
Publication Abstract from PubMed
TrkH belongs to a superfamily of K(+) transport proteins required for growth of bacteria in low external K(+) concentrations. The crystal structure of TrkH from Vibrio parahaemolyticus showed that TrkH resembles a K(+) channel and may have a gating mechanism substantially different from K(+) channels. TrkH assembles with TrkA, a cytosolic protein comprising two RCK (regulate the conductance of K(+)) domains, which are found in certain K(+) channels and control their gating. However, fundamental questions on whether TrkH is an ion channel and how it is regulated by TrkA remain unresolved. Here we show single-channel activity of TrkH that is upregulated by ATP via TrkA. We report two structures of the tetrameric TrkA ring, one in complex with TrkH and one in isolation, in which the ring assumes two markedly different conformations. These results suggest a mechanism for how ATP increases TrkH activity by inducing conformational changes in TrkA.
Gating of the TrkH ion channel by its associated RCK protein TrkA.,Cao Y, Pan Y, Huang H, Jin X, Levin EJ, Kloss B, Zhou M Nature. 2013 Apr 18;496(7445):317-22. doi: 10.1038/nature12056. PMID:23598339[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Cao Y, Pan Y, Huang H, Jin X, Levin EJ, Kloss B, Zhou M. Gating of the TrkH ion channel by its associated RCK protein TrkA. Nature. 2013 Apr 18;496(7445):317-22. doi: 10.1038/nature12056. PMID:23598339 doi:http://dx.doi.org/10.1038/nature12056
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