1u2z

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[[Image:1u2z.gif|left|200px]]
[[Image:1u2z.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1u2z |SIZE=350|CAPTION= <scene name='initialview01'>1u2z</scene>, resolution 2.20&Aring;
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The line below this paragraph, containing "STRUCTURE_1u2z", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Histone-lysine_N-methyltransferase Histone-lysine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.43 2.1.1.43] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= DOT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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-->
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|DOMAIN=
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{{STRUCTURE_1u2z| PDB=1u2z | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u2z OCA], [http://www.ebi.ac.uk/pdbsum/1u2z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u2z RCSB]</span>
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}}
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'''Crystal structure of histone K79 methyltransferase Dot1p from yeast'''
'''Crystal structure of histone K79 methyltransferase Dot1p from yeast'''
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[[Category: Yang, Z.]]
[[Category: Yang, Z.]]
[[Category: Zhang, X.]]
[[Category: Zhang, X.]]
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[[Category: histone methyltransferase]]
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[[Category: Histone methyltransferase]]
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[[Category: nucleosome]]
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[[Category: Nucleosome]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:41:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:04:42 2008''
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Revision as of 07:41, 3 May 2008

Image:1u2z.gif

Template:STRUCTURE 1u2z

Crystal structure of histone K79 methyltransferase Dot1p from yeast


Overview

Methylation of Lys79 on histone H3 by Dot1p is important for gene silencing. The elongated structure of the conserved core of yeast Dot1p contains an N-terminal helical domain and a seven-stranded catalytic domain that harbors the binding site for the methyl-donor and an active site pocket sided with conserved hydrophobic residues. The S-adenosyl-L-homocysteine exhibits an extended conformation distinct from the folded conformation observed in structures of SET domain histone lysine methyltransferases. A catalytic asparagine (Asn479), located at the bottom of the active site pocket, suggests a mechanism similar to that employed for amino methylation in DNA and protein glutamine methylation. The acidic, concave cleft between the two domains contains two basic residue binding pockets that could accommodate the outwardly protruding basic side chains around Lys79 of histone H3 on the disk-like nucleosome surface. Biochemical studies suggest that recombinant Dot1 proteins are active on recombinant nucleosomes, free of any modifications.

About this Structure

1U2Z is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure of the conserved core of the yeast Dot1p, a nucleosomal histone H3 lysine 79 methyltransferase., Sawada K, Yang Z, Horton JR, Collins RE, Zhang X, Cheng X, J Biol Chem. 2004 Oct 8;279(41):43296-306. Epub 2004 Jul 29. PMID:15292170 Page seeded by OCA on Sat May 3 10:41:35 2008

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