1j5h

From Proteopedia

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Current revision

Solution Structure of Apo-Neocarzinostatin

Structural highlights

1j5h is a 1 chain structure with sequence from Streptomyces carzinostaticus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 44 models
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NCZS_STRCZ NCS has antibiotic activity (for Gram-positive bacteria) and antitumor activity (for certain mouse tumors). NCS binds non-covalently to a chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore binds to DNA as a weak intercalator and causes single- and double-strand breaks.

Publication Abstract from PubMed

The natural complex Neocarzinostatin comprises a labile chromophore noncovalently bound to an 11.2 kDa protein. We present the first high-resolution structure of a novel complex derived from the recombinant apoprotein bound to a non-natural synthetic chromophore. Fluorescence and nuclear magnetic resonance spectroscopy were used to probe the strength and location of binding. Binding occurred in a location similar to that observed for the chromophore in the natural Neocarzinostatin complex, but with a distinct orientation. These results provide structural evidence that the apoprotein can readily accommodate small druglike entities, other than the natural chromophore within its binding cleft. The clinical use of the natural complex described by others, together with the results reported here, suggests potential applications for small molecule binding by apo-Neocarzinostatin.

Solution structure of a novel chromoprotein derived from apo-neocarzinostatin and a synthetic chromophore.,Urbaniak MD, Muskett FW, Finucane MD, Caddick S, Woolfson DN Biochemistry. 2002 Oct 1;41(39):11731-9. PMID:12269815^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Urbaniak MD, Muskett FW, Finucane MD, Caddick S, Woolfson DN. Solution structure of a novel chromoprotein derived from apo-neocarzinostatin and a synthetic chromophore. Biochemistry. 2002 Oct 1;41(39):11731-9. PMID:12269815

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

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Retrieved from "http://52.214.119.220/wiki/index.php/1j5h"

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 == Structural highlights ==
 <table><tr><td colspan='2'>[[1j5h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J5H FirstGlance]. <br>
-</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 44 models</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j5h OCA], [https://pdbe.org/1j5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j5h RCSB], [https://www.ebi.ac.uk/pdbsum/1j5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j5h ProSAT]</span></td></tr>
 </table>

1j5h

From Proteopedia

Current revision

Solution Structure of Apo-Neocarzinostatin

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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